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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Major results were summarized as follows:(1) A total of 58 tree species from 53 genera in 36 families among which 8 species were Rosaceae, 5 species were Liliaceae, 5 species were Eriaceae, 3 species were Ranunculaceae, 3 species were Compositae, 2 species were Cupressaceae, 2 species were Caprifoliaceae, 2 species were Umblliferae, and other 28 tree species were only one receptively. These were recorded in the 8 plots of the A. georgei var. smithii forest;(2) Number of families, genera, species and Margalef index correlated negatively with altitude (P.05), with a peak at 3600 m.(3) Shannon-Wiener index correlated negatively with altitude (P.01), and maintained stable at the altitudes between 3700~4100 m, evenness with altitudes, however, this trend was insignificant.(4) Jaccard index increased sharply with increasing altitude at the altitudes between 3600~4100 m, and was lower between different vegetation types at the altitudes between 4100~4200. Cody index β(subscript c decreased with an increasing altitude, but there were 2 troughs between 4000~4100 m and 4200~4300 m.(5) Maximum tree height H(subscript max and HH(subscript max=37 mcorrelated negatively with altitude (P.05); but basal area and BA BA(subscript max=5.3m^2 correlated with altitudes, however, this trend was insignificant.

结果表明：(1)在调查的8个急尖长苞冷杉林样地内共有植物58种，分属于36科53属，其中植物较多的科有蔷薇科8种、百合科5种、杜鹃花科5种、毛茛科3种、菊科3种、柏科2种、忍冬科2种、伞形科2种，其余的28科各只有1个种；(2)物种科、属、种数、Margalef指数D(下标 M与海拔存在显著的负相关性(P.05)，在分布急尖长苞冷杉最低海拔3600m处出现物种丰富度的最大值；(3)多样性指数与海拔之间有极显著的负相关性(P.01)，并且在3700~4100m之间多样性指数保持稳定；均匀度指数与海拔梯度之间存在负相关性，但不显著；(4)Jaccard指数C(下标 j在海拔3600~4100m随海拔的升高而升高，在生境过渡带的4100~4200m之间Jaccard指数C(下标 j较低；Cody指数β(下标 c随海拔的升高呈下降的趋势，但在4000~4100m和4200~4300m 海拔区间出现2个低谷；(5)最大树高H(下标 max和最大胸径DBH(下标 max与海拔之间存在显著负相关性(P.05)；胸高断面积之和和立木密度与海拔之间存在负相关性，但不显著。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠，生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01，P.05)，生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %；⑵与空白对照组相比，生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01)，生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上；⑶与S180荷瘤组相比，生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势，其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05)；⑷与S180荷瘤组相比，生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01)，表明其可提高荷瘤小鼠体液免疫功能；⑸与对照组相比，生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01)，表明其可提高荷瘤小鼠细胞免疫功能。

Wicked, irreverent, smart, and full of self-depreciating humor, this queen of grime rap pillages her autobiography on "Those Were the Days," deconstructs nationalism on "My England," raids Mother Goose on "Public Warning," and then drops a gauntlet with a loud clattering sound, making listeners laugh out loud with the snotty, but laughable challenge of "Love Me or Hate Me."

卑劣的，不敬的，聪明的，和充满的自我贬值的幽默，世俗尘垢影响的这一位天后，表现在她的自传在& Those Were The Days &里，体现在& My England &上的民族主义，奇猎在& Public Warning &中的Mother Goose，然后以一种独特的声音向世人宣布，使听者因为她的狂放不羁而嗤笑，除了在& Love Me or Hate Me&的可笑尝试。

The word "be" has eight forms: be, is, am, are, was, were, been, being.

这种动词有八种形式：be， is， am， are， was， were， been， being。

First,she hit the red carpet in a figure-hugging Stephane Rolland gown withintricate appliques, quickly changing into a gorgeous Atelier Versaceleather dress for her roof-shaking performance of "If I Were a Boy" andthe Alanis Morissette classic "You Oughta Know."

第一件是伴随她走上格莱美红地毯的、使她曲线毕露的Stephane Rolland晚礼服。但随后马上就又为了其后表演震撼全场，换上一件无比诱惑、让人猛喷鼻血的Atelier Versace褶皱小皮裙，载歌载舞的演唱了&If I Were a Boy&和&You Oughta Know&，让下面观众的尖叫几乎爆棚。

Since, in fact, there were more than can possibly have been used in context, it is probable that the clausulae came to be performed independently, either in other parts of the mass, or in private devotions.

因为事实上在此时期，这种特别的风格在有比这种可能性更多的运用（were more than can possibly have been used in context）。克劳苏拉可能得到了独立的表演--在一些弥撒、 private devotion中。

During the 1600's skilled shoemakers scarce were in what is now the United States.

分析：C错，应改为were scarce。表语应该放在系动词后面。

If one,replica tag heuer, what about "be","am","are","is","was","were"?

若作一个计算，那么&be&、&am&、&are&、&is&、&was&、&were&该如何算呢？

What were you doing when the UFO arrived ?（2）I was sitting in the barber's chair.

动词be的过去式was /were＋动词的现在分词构成。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。