查询词典 subtractive process

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

UniESTs were obtained, 400 of them gained from the subtractive library of stage of proembryo mass and 468 from that of somatic embryo maturation.

上述3种抗氧化酶通过相互配合，协同调节胚性细胞的分化和发育。4。

Functionally known genes were obtained after Blastx analysis, 237 of them were from the subtractive library of stage of proembryo mass and 279 from that of somatic embryo maturation.

成功构建了Y35体细胞胚原胚团阶段和成熟阶段的差异表达基因文库，获得了868个差异表达的UniESTs，其中原胚团阶段400个，体细胞胚成熟阶段468个。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Besides, we firstly confirmed at the molecular level that P450arom may play an important role in the natural sex change of protandrous fish and revealed that up-regulation of the transcript levels of P450arom during the course of natural sex change of black porgy is not regulated via FTZ-F1.;sturgeon sex related and sex linkage genes by candidate gene approach and suppression subtractive hybridization technique and expression patterns of these genes were also studied.

首次直接在分子水平证明芳香化酶基因可能参与了雌雄同体鱼类性别逆转的调控，而黑鲷性别逆转期卵巢中芳香化酶的表达量上调不是由FTZ-F1基因调控的；同时主要利用候选基因法和抑制差减杂交搜寻史氏鲟性别决定或性别特异性基因，并研究其表达情况。

This study has achieved the following purposes,observed effects of different levels of noise exposure on the auditory function and the hearing organ of guinea pigs,explored optimal noise exposure profiles for research of NIHL susceptibility,established NIHL susceptibility model,148 differentially expressed genes of cochlea between NIHL susceptible and unsusceptible guinea pigs were screened by using suppression subtractive hybridization.The results of bioinformatics analysis showed that there are 34 sequences whose homology were larger than 80% and match sequence larger than 100 bp.

本研究观察了不同噪声暴露量对豚鼠听觉功能和听觉器官结构的影响，探讨了NIHL易感性研究的最佳噪声暴露方案，建立了NIHL易感豚鼠模型，利用抑制性消减杂交技术筛选NIHL易感与不易感豚鼠耳蜗的差异表达基因片段148条，生物信息学结果显示同源性大于80%且匹配碱基大于100 bp的片段共34条。

Message R NA was extracted from those cerebrums. Suppression subtractive hybridization was conducted accord ing to manufacture's manual.

提取两组组织的mRNA，按照抑制性差减杂交试剂盒说明进行实验。

To exert the use of high sensitive CCD and improve the performance of instruments and the quality of pulsatile signal of spectrum,the paper based on linear CCD of buried channel IL-C6-2048C,the output of which is subtractive polarity and discrete analog signal,and intermixes the high amplitude of reset-pulse,designs the circuit to pre-treat properly to the output's signal.

为了充分发挥高灵敏度CCD的作用，提高脉动光谱信号的质量和仪器的性能，本文在埋沟道型IL-C6-2048C线阵CCD基础上，根据CCD的输出信号是负极性的离散模拟信号并且混杂有幅度较大的复位脉冲干扰的特点，设计电路对CCD输出信号进行适当的预处理。

By Nothern blot, this gene band located between 1383-1908bp maker, length of which was same with different gene obtained by subtractive hybridization. The results showed that the obtained wnt6 gene was a cDNA fragment with full open reading frame, which could be useful for further studies.

提取样本总RNA，与地高辛标记的Wnt6探针进行杂Northernblot杂交，结果阳性杂交带位于1383-1908bp处，与差异片段大小一致，验证了该基因确为全长cDNA片段，具有完整的开放读框，可以用于进一步研究。

The father that if work,mad father, love annoys, disposition is too indrawn the be short ofing that the father of Mu Ne, resign oneself to adversity causes father easily to love is broken; Communicate time effectively between father and son, Mu Zizhi to be less than a hour on average to wait a moment every week, it is the cause that causes the child to become addiction.

如工作狂的父亲、爱愤怒的父亲、性格过于内向木讷、逆来顺受的父亲都易造成父爱的缺失；父子、母子之间的有效沟通时间每周平均小于一小时等等，都是造成孩子成瘾的原因。

These are vandal proof and they come with a locking mechanism.

这是野蛮的证据，他们来到了锁机制。