查询词典 subtractive process

METHODS: The collagen type Ⅰ gene was cloned by the improved subtractive hybridization, and the fragment of collagen type Ⅰ cDNA was labeled by digoxin and was hybrided by in situ hybridization on the section of capsule of normal and cataractous lens.

用改良的消减杂交法克隆Ⅰ型胶原基因，地高辛标记Ⅰ型胶原cDN####段制作探针，用原位杂交法研究其在晶状体前囊膜切片上的表达，并作统计学处理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

In this research, we attempted to construct an SSH (suppression subtractive hybridization) library using the leaves of wild H. villosa (Gh21) inoculated by Erysiphe graminis and its susceptible EMS-induced mutant M14S with no inoculation.We screened 218 positive clones in the SSH library, and gained 60 ESTs after sequencing.

本研究应用抑制性消减杂交技术，以野生型簇毛麦(Gh21)及其感病突变体(M14S)为材料，建立白粉菌诱导的叶片的SSH文库，获得218个阳性克隆，对其中部分克隆测序，获得60个EST序列。

In this report, subtractive hybridization coupled with polymerase chain reaction was used to identify genes related to erythroid terminal differentiation from FVA cells cultured with EPO for 36 hours.

因此我们选用了FVA细胞为材料，用减除杂交与PCR相结合的方法，克隆小鼠红系细胞终末分化阶段的红细胞分化相关基因，得到了一个红系细胞的中晚幼阶段差异表达的cDNA片段并对其进行了初步的功能研究。

Firstly, the structure of ANFIS is decided by subtractive clustering; then the premise parameters and consequent parameters of ANFIS are trained by hybrid learning algorithm; finally, related factors that impact futural daily electricity price are input into the trained ANFIS to forecast electricity price.

首先采用减法聚类方法确定自适应模糊神经网络的结构，然后利用混合学习算法训练该网络的前件参数和结论参数，最后将影响未来日电价的相关因素输入到训练好的自适应模糊神经网络中进行电价预测。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

ERIC, a pair of rep-PCR primers, can amplify NSa-specific band. Using the amplification band patterns of two pairs of primers, which are REP and ERIC, six types of male sterile cytoplasm can be distinguished. 2. A cDNA library for CMS-associated genes was constructed by suppression subtractive hybridzation with the sterile line and its isonuclear maintainer.

利用特异引物扩增的结果表明NSa不育胞质与Pol、Ogu不育胞质不同，一对rep-PCR引物ERIC能扩增到NSa的特异带，而且REP和ERIC两对引物相结合能将NSa不育胞质与油菜原有的不育细胞质区分开，从分子水平上证明了NSa不育胞质的新颖性。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

Smoking cigarettes adds to the Computer Desk Computer manufacturer computer mouse Computer Computerized collar knitter machine Conduit Bodies construction board Container Bag Control Arm Cooking Thermometer general levels of air pollution as well as damaging our health.

吸烟不仅对健康有害，而且增加空气污染程度。香烟雾含有多种有害物质：不仅有致癌化学物质、有某种能阻止氧气进入血液的气体，还有引起血压增高、心速加快的被称为尼古丁的物质。

He is one of my favorite actors.

他是我最喜欢的男演员之一。