查询词典 structural gene

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

Mitochondrial 16S rRNA gene and COⅠ gene fragments were amplified and sequenced from 4 wild populations of Portunus trituberculatus. Respectively, 524bp and 658bp long partial gene fragments of the 16S rRNA gene and the COⅠ gene were obtained. A high percentage of A+T base composition in the two sequences was observed, which was similar to the results of studies on drosophila, shrimp, crab and other invertebrates.

对分别采自辽东湾、莱州湾、海州湾和舟山的三疣梭子蟹4个野生群体的线粒体16S rRNA和COⅠ基因片段进行了扩增和测序，分别得到长度为524bp和658bp的片段。2段序列的碱基组成均显示较高的A+T比例（16S rRNA基因70.8%，COⅠ基因63%），这与果蝇、虾类、蟹类等无脊椎动物的16S rRNA和COⅠ基因片段研究结果相似。

On the bases of two parts above, in chapter 3, the ethical issues about the HGP are analyzed:①gene privacy invades human beings' autonomies;② gene discrimination breach human beings' dignity;③gene eugenics will result in human's lose;④"gene determinism" may result in human right's damage;⑤ gene patent can not be defended morally;⑥gene therapy may invade sufferers' autonomies;⑦issues about gene crime and gene weapon.

在具备了前两部分的基础上，在本文的第三部分对人类基因组计划进行了价值伦理分析：①基因隐私侵扰人的自主权；②基因歧视违背了人的尊严；③基因优生将导致人性的丧失；④&基因决定论&将导致对人类权利的侵犯；⑤基因专利在道义上是不能辩护的；⑥基因治疗可能违背患者的自主权；⑦基因犯罪和基因武器问题。

The hygromycin resistance gene is a marker gene to help sort out the transgenic mushroom cells from the non-transgenic cells, Dr. Romaine explained. What we are doing is taking a gene, as for example a drug gene, that is not part of the mushroom, and camouflaging it with regulatory elements from a mushroom gene.

潮霉素基因是一个标记物，它能筛选转化了和未转化的蘑菇细胞，Romaine教授解释说，我们做的就是得到这样一个基因，例如它是一个药物基因，它不是蘑菇基因的本身的组成部分，它伪装成蘑菇基因要做一个调整。

After the RT-PCR detection of the total RNA from heart tissue, we found that the CD59 gene and MCP gene were expressed. 4. The CD59 gene and MCP gene were mixed with liposome, then used to produce transgenic swine by 3 methods. Firstly, the gene/ liposome complex was directly injected into male swine's testis. The transfected males copulated with 8 females each 44 days later.

其中睾丸注射法处理公猪1头，44d后配母猪8头，7头受孕，产仔73头，结果检测到PCR阳性猪3头(阳性率4.3%)；输精管注射法处理公猪3头，8d后配母猪3头，0头受孕；外源基因/精子共孵育法处理公猪4头，处理母猪4头，1头受孕，产仔11头，结果检测到PCR阳性猪1头(阳性率9%)。

Meanwhile, in order to explore the stability of gene expression and the properties of gene-modified bone marrow cells further, we have observed the capability of reconstituting hematopoietic system of gene-modified and the expression of transduced gene in vivo by using of BMT to engraft lethally irradiated mouse with the gene-modified bone marrow cells.

同时，为进一步探讨转导细胞基因表达的稳定性以及生物学特征，本实验通过骨髓移植的方法，将基因标记骨髓细胞移植给受致死量照射小鼠，观察了基因转导细胞重建体内造血的能力，以及造血重建后外源基因在脾结节及骨髓细胞的表达。

This thesis with lie to lead to acquire through the agriculture rod bacteria of turn the gene paddy rice T0 and a seeds in order to experiment the material, the adoption PCR examination and Southerns are miscellaneous to hand over the examination to combine together of method, pass the examination conversion plant posterity in the HPT( the Hygromycin phosphotransferase) gene, study the outside source gene in turn the gene paddy rice posterity of separation circumstance and heredity stability;Pass the influence to a form of form of the conversion plant carries on statistics to learn the analysis, studying the outside source gene to the conversion the sex form of plant.

本论文以通过农杆菌介导获得的转基因水稻T0和T1代种子为试验材料，采用PCR检测和Southern杂交检测相结合的方法，通过检测转化植株后代中HPT基因，研究外源基因在转基因水稻后代中的分离情况和遗传稳定性；通过对转化植株的表型性状进行统计学分析，研究外源基因对转化植株的性状的影响。

This study was conducted to clone the sheepβ-lactoglobulin gene and the human myelomonocytic surface differentiation antigen CD14 gene (hCD14) by PCR, and this two gene were fused to construct oBLG-hCD14 fuse gene, this fuse gene were microinjected into pronuclei of mouse eggs to produce transgenic mouse.

本研究拟利用PCR和分子克隆技术，首先获得绵羊β-乳球蛋白基因调控序列片段和人CD14基因（hCD14）全程编码序列，并构建oBLG-hCD14融合基因，最后利用微量注射法将融合基因导入小鼠受精卵以产生转基因小鼠。

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因，包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因，在缺氮诱导条件下转录水平有明显上调。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

This matter overthrew all basises I learned from Sunday school .

这件事把我从周日学校学到的所有道理都颠覆了。

It's one of the cruel ironies of aging: if you want to remember your favorite restaurants, the less you should eat at them.

这是一个老化的残酷讽刺：如果你想记住你最喜欢的餐馆，你应该更少吃到它们。