查询词典 structural gene

It is suggests that Doc -1R gene has particular importance in maintaining the function of the tissues and Doc-1R gene perhaps is a house-keeping gene.

小鼠Doc-1R基因的mRNA在各组织均为阳性表达，因此提示，Doc-1R基因可能是一种对维持正常细胞功能具有重要意义的管家基因。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

An enriched PCR fragments with less complexity were obtained and mainly expressed in the denervated hippoampus After cloning into the plasmid vector, 46 clones were initially screened and subjected to autosequencing By comparison with the EMBO gene databases, 21 gene fragments of different types were obtained with 11 similar to genes of known function and 10 similar to ones of unknown function It is inferable that the altered gene expressions of hippocampus following the entorhinally denervation may underlie the specfic reinnervation and the formation of synapses by transplanted entorhinal neurons with host neuropil within the denervated target areas.

初步挑选出的46个转化后的阳性克隆，经点杂交筛选和基因序列分析，与Gene Database同源性比较后，得到21种基因片段，其中功能已知的有11种，功能未知的有10种。结果表明海马在去内嗅皮层后某些基因表达的改变，这可能是移植胚胎内嗅皮层神经细胞轴突特异支配去神经靶区和重建突触联系有关。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子，本研究构建了带报告基因的载体并检测了其启动子，增强子和减弱子的功能，以此为基础，进一步构建了MBP异位表达载体，为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

According to Mather and Jinks model, using generations mean analysis m eth od, the various kinds of gene effects and its percentage of gene-effect-square s um were estimated,and comparative analysis on the gene effects and the importan ce t o inheritance of characters was carried out between the supernumerary spikelet l ines.

黄毅以主穗小穗数在25～30个的二个普通小麦多小穗品系85DH5015、SW94-30921 与三个普通小麦品种组配成6个组合的P1、P2、F1、F2、B1、B2为材料，用世代均值法分析了6个性状的遗传模型并估算了各类基因效应值，比较分析了两个不同来源多小穗小麦品系六个性状各类基因效应的异同及其相对重要性。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

In conclusion,this research has gotten some significant results:firstly Kail gene is highly related with the metastasis potential of colorectal carcinoma cells,secondly mutation of Kail gene,methylation of CpG islands in the promoter and abnormal expression of p53 have no important effects in the down-regulation of Kail gene.

本研究结果表明：Kai1基因与大肠癌细胞转移潜能有关；基因点突变、启动子CpG岛甲基化与p53异常在Kai1基因表达下调中不起重要作用。

The production of human alpha lactalbumin was 0.64 g/L approximately. The results indicate that the bovine mammary epithelial cell line have the ability of expressing exogenous gene, the 5′ flanking sequence of bovine αS1 casein gene has the effect of regulating gene expression specifically and the vector αS1-LA-psv which was constructed can coexpress alpha lactalbumin and β-galactosidase.

实验结果表明，建立的奶牛乳腺上皮细胞系具有外源基因表达活性，得到的牛αS1-酪蛋白5′调控序列能作为启动子指导外源基因的表达，构建的真核表达载体能在体外培养的牛乳腺上皮细胞中同时表达人α-乳白蛋白和β-半乳糖苷酶。

In comparison of the NS gene analysis, changes of the amino acid sequences are lesser than those in the HA gene. The phylogenetic analysis of NS gene is not form different linage between B/Taiwan/3143/97 and B/Taiwan/21706/97, and all isolates were closed to each other.

由HA基因序列的比较中发现，新的分离株的突变位置与B/Taiwan/3143/97大部分都类似，但突变点相较之下数目较少；而在演化树的分析中，新的分离株会与B/Taiwan/3143/97聚集，形成一新的群集。

Results indicated that bar gene was integrated into wheat genome of T1 and T2 ,and bar gene expressed stably. Separation of bar gene in T1 and T2 complied with Mendel's law. Agronomic traits of yields and quality have no notable differences between bar-transgenic wheat and controls.

证实抗除草剂bar基因已经整合到小麦基因组中，并能稳定表达；遗传分析显示bar基因能稳定的遗传给后代，符合孟德尔遗传规律；在产量及品质等主要农艺性状方面，转bar基因小麦与对照相比无显著差异。

We stopped by the old fashion ice cream parlor Manhattan Beach Creamery for some refreshment. The "No Shoes, No Shirt, No Problem" sign on the wall epitomizes the beach lifestyle.

我们去商店区的一家老式冰淇淋店坐坐，墙上挂著「没鞋，没上衣，没问题」的牌子完全捕捉了海滩生活的随性及风格。

"If therefore", argues Bellarmine we can offer our prayers and our satisfactions in behalf of those detained in purgatory, because we are members of the great body of Christ, why may not the Vicar of Christ apply to the same souls the superabundant satisfaction of Christ and his saints--of which he is the dispenser?

" "因此，如果"，认为贝拉明（德indulgentiis ，十四）"我们可以提供我们的祈祷和我们满意的代表那些被拘留在炼狱，因为我们是成员的伟大基督的奥体，为什麼可能不是牧师基督适用於同样的灵魂满意的过剩是耶稣和他的圣人-他是在配药？