查询词典 structural gene

To understand the mononuclear cells in such a state of gene expression, we use gene chip technology to investigate the differentiation of monocyte gene expression.

为了理解单核细胞在这种状态下的基因表现，我们运用基因芯片的技术来调查单核细胞的分化的基因表达。

It were analyzed DNA polymorphism in baby rats between outbred Wistar and SD rats with 30 microsatellites DNA loci of PCR amplification. And was breeding baby inbred rats using high and middle of Coefficient of similarity with female SD rats of F0. It was indicated that there were hybrid and polymorphism loci, there weren't homo-gene loci of F2. It were monomorphism homo-gene for 27, rate of homo-gene loci for 90%.

利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析，仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点，没有纯合位点；到F9代时基因纯合位点达27个，纯合基因位点率为90%。

The scope for horizontal gene transfer is essentially the entire biosphere, with bacteria and viruses serving both as intermediaries for gene trafficking and as reservoirs for gene multiplication and recombination (the process of making new combinations of genetic material).

方舟子的这一观点来自侯女士的另一个证据就是，他在作于2005年1月的《答〈中国青年报〉记者刘县书的批评》中写道：HGT"是近十几年来才引起生物学界注意的遗传现象"。

Methods: The expression of EOMES gene, ETS-1 gene and MEF (myeloid Elf-1 like factor) gene in 25 cases of N-NK/T-L and 25 control cases including 11 cases of B cell lymphoma (BCL, 6 cases of T cell lymphoma, 3 cases of normal spleen, 5 cases of chronic nasopharyngitis were detected by tissue microarray and in situ hybridization.

通过对EOMES，ETS-1和MEF基因进行克隆、探针制备和组织芯片制备，对25例N-NK/T-L以及同部位11例B细胞淋巴瘤、6例T细胞淋巴瘤、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。

Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.

bfal在骨形成和成骨细胞分化过程中起着重要的作用，是调控成骨细胞特异性基因表达的主控基因，该基因只在骨形成相关细胞中表达，如果使其在非成骨细胞中表达，该细胞将出现成骨细胞的特征。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Objective: To study the relationship between the gene expressions of ornithine decarboxylase and lung cancer in the elder from the level of mRNA and protein, and therefore to establish the theoretical basis for gene diagnosis and gene therapy in the future.

目的：从mRNA和蛋白质水平探讨ODC基因表达和老年人肺癌的关系，为肺癌基因诊断和基因治疗的研究提供理论依据。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

SW480 and SW620 cells were also used to study the gene regulation with high carbohydrate intake, where it was shown that during glycolysis eight genes, HK1 (nuclear gene encoding mitochondrial protein, transcript variant 1), GPI, GAPD (glyceraldehyde-3-phosphate dehydrogenase), PGK1 (phosphoglycerate kinase 1), PGK2 (phosphoglycerate kinase 2), ENO2 (enolase 2), PKM2 (pyruvate kinase, muscle, transcript variant 2) and GLUT1 (facilitated glucose transporter, member 1) are over-expressed. In our study, we also found that during hypoxia of cancer tissues the resulting up-regulation of HIF-2α(hypoxia-inducible factor 2, alpha subunit) would in turn up-regulate the GLU1 gene, further activating glycolysis.

另一方面，在高碳水化合物的调控研究上，吾人也利用SW480和SW620细胞株分析证实醣解水解活化过程中，其六碳醣激酶-1(HK1)、葡萄糖磷酸异构酶、3-磷酸甘油醛脱氢酶、磷酸甘油酸激酶-1(PGK1)、磷酸甘油酸激酶-2(PGK2)、烯醇酶-2(ENO2)、丙酮酸激酶-2(PKM2)以及葡萄糖输送蛋白-1(GLUT1)等共有8个基因是连续上升的过度表现；此外，吾人也发现当癌细胞在缺氧的组织中，其缺氧转录子-2α基因(HIF-2α)表现上升后，会活化GLU1基因上升，进一步活化醣解代谢的进行。

This is the inheritance of the tribe of the children of Issachar according to their families, the cities and their villages.

19：23 这些城并属城的村庄就是以萨迦支派按着宗族所得的地业。

I've have enough of your garbage.

我听了你的废话。