查询词典 structural gene

The DNA fingerprints of 4 biotypes of GM population from 7 locations were analyses by AFLP.Base on the fine mapping of resistance gene Gm6 against all 4 Chinese GM biotypes by RAPD and SSR methods respectively,the STS markers and SSR markers linked to gene Gm6 were used for breeding through marker assisted selection.Some new resistant germplasm with Gm6 gene were created.And 6 cultivars and 6 lines of two-line hybrid rice and 1 line of three-line hybrid rice against GM were bred and extended to the farmer.The technique system of MAS for resistant varieties against Asian rice gall midge was set up at Guangdong province of China.

用AFLP方法对从中国广东省7个地点采集的4个生物型的DAN指纹进行分析；在对用RAPD和SSR技术分别对抗中国4个稻瘿蚊生物型的基因Gm6精细定位基础上，用与Gm6紧密连锁的STS和SSR标记开展分子标记辅助育种，创造了一批抗稻瘿蚊的新种质，包括育成了6个栽培稻和6个二系杂交稻和1个三系杂交稻并在农户试种，在中国广东成功地建立了分子标记辅助选育抗稻瘿蚊品种的技术体系。

When the deduced amino acid sequence of the new gene was compared with those of other glutelin genes, they showed about 80%, 70% and 60% between the new gene and GluB-4, GluB-1 or GluB-2, GluA, respectively, suggesting that the new glutelin gene belonged to the subfamily, GluB.

克隆测序和同源性分析比较结果表明，由该基因推导的氨基酸序列与GluB-4相似性近80％，与GluB-1，GluB-2的相似性近70％，而与GluA亚族各谷蛋白的相似性不到60％。

Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.

利用随机引物逆转录合成互补脱氧核糖核酸，根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp,PCR testing technology were employed to test the Hu-IFN-α gene's genetic expression in its cross F1 and gynogenesis F1 generation.

为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp, PCR testing technology were employed to test the Hu-IFN-α gene＇s genetic expression in its cross F1 and gynogenesis F1 generation.

文章摘要：为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

Three pairs of primers were designed according to nuc gene of SA, hly gene of LM and hemolysin gene of BC, and then the single PCR was performed to detect specificity and sensitivity.

根据SA的nuc基因、LM的hly基因和BC的hemolysin基因，设计合成三对特异性引物，然后分别进行单基因PCR反应的特异性和灵敏性验证。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

结果 大肠癌标本p53基因突变率为50%；其中突变组肿瘤细胞凋亡率(22.11%)明显低于未突变组细胞凋亡率(40.57%)，(P＜0.05)；突变组异倍体细胞百分率(占76.9%)明显高于未突变组(占46.2%)，(P＜0.05)；异倍体肿瘤细胞凋亡率为34.0%，二倍体肿瘤细胞凋亡率为40.7%，二者差异无显著性(P＞0.05)。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didn\'t changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didnt changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

The result of WISH showed that the trascription of hira gene distributed widely in the early stages of embryos development and centralized to head, heart and the end of tail. Hira gene might affect the development of brain and heart. The expression characterization of colored crucian carp Cahira gene in different tissues and different stages of embryogenesis were also investigated by RT-PCR.

胚胎原位杂交显示，Hira基因的转录本在银鲫胚胎早期广泛分布，而在胚胎后期主要集中于头部、心脏及尾芽尖部，说明也对其脑部和心脏的发育有影响。

The quality of both red and white wine depends very much on the wine maker talent and on the quality of the grapes.

他的红酒和白酒的品质，根据酿造者的能力和葡萄的质量，各有差异。

No, I don't want to get drunk before my next match.

" "不，我不想让我的下一场比赛前喝醉了。