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Methods:The rat intra-pulmonary arterywas separated carefully and cut into small pieces.After being treated by collagenase Ⅰ(0.2%) about 8 hours,those tissue pieces were cultivated in DEME culture medium with 20% calf serum.

取大鼠肺分支动脉，先切成小块进行胶原酶的消化，约8 h，而后用含20%小牛血清的DEME培养基贴块培养。

Methods Proteins and peptides in CSF were separated by two-dimensional gel electrophoresis（2DE） and ultra-performance liquid chromatographer and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS） and electrospray ionization to study differential proteomic expression in the cerebrospinal fluid between patients with Demy- elinative disease and with other neurological diseases.

方法以中枢神经系统脱髓鞘疾病患者的脑脊液为研究对象，分别采用固相pH梯度双向凝胶电泳分离总蛋白质，凝胶经银染显色后，用图像分析软件进行比较分析、识别差异表达的蛋白质，离线应用基质辅助激光解析电离飞行时间质谱得到相应的肽指纹图谱；同时用超高压液相色谱分离酶解肽段，在线采用电喷雾四极杆飞行时间质谱鉴定肽段氨基酸序列；搜索数据库鉴定部分蛋白质。

The diagnostic features for the species include deep excavation on the posteroventral margin of the premaxilla, a diastema between the premaxillary and maxillary teeth, a a number of many pits and ridges on the anterolateral surface of the antorbital fossa, the posterolateral process of the parietal being long and sharply posteriorly directed, the column ar -like margin of the pterygoid process of the quadrate, the bifurcated posterior margin of the dentary, a distinctive groove posterior to the anterior carina on the medial surface of the premaxillary tooth crowns, absence of ventrolateral processes on intercentrum of t he atlas, T-shaped centrum of the first caudal in ventral view, transverse process on anterior caudals with rounded , and strongly anteroposteriorly expanded distal end, presence of two fossae separated by an oblique ridge on the lateral side of the anterior chevrons near the articular end, and pubis strongly curved psoteriorly.

这一属种的主要鉴定特征包括：前上颌骨后腹缘有一深凹、前上颌齿和上颌齿之间有一齿缺、眶前窝外表面前部有许多坑窝和脊、顶骨侧后突伸向后方、方骨的翼骨支边缘柱状、靠近前上颌齿齿冠舌面前缘有槽、环椎间椎体外腹突缺失、第一尾椎椎体腹视 T 形、前部尾椎横突远端前后向强烈扩展呈圆形、前部脉弧近端外侧面有两个被一斜脊分离的窝以及耻骨强烈向后弯曲。通过分析千禧中国鸟龙的96个骨骼学特征，发现其中21个特征支持奔龙类与鸟类的亲缘关系最近，9个特征支持奔龙类与伤齿龙类亲缘关系最近，25个特征支持奔龙类、伤齿龙类和鸟类亲缘关系较为接近。

Marlin and Nemo become separated in the Great Barrier Reef when Nemo is unexpectedly taken far from home and thrust4 into a fish tank in a dentist's office overlooking Sydney harbor.

在大堡礁，尼莫与父亲走散了，被意外地带到了离家很远的地方，在俯瞰悉尼港的一个牙科诊所，他被塞进了一个鱼缸里。

In the era of knowledge-driven economy, we cannot be separated from the computer network. But the computer virus, with its infectivi ty and destructibility , is playing havoc with our normal lives.

知识经济时代，人们的生产和生活已经离不开计算机及计算机网络，但计算机病毒却以它极强的传染性和严重的破坏性危及着我们的正常生活。

The light source detecter makes used of the variation of the separated voltage by the resistivity of photosensitive resistance input to the singlechip. If the voltage higher than a worth, the singlechip will regard it as high voltage state, it means that the light source has been detected. Otherwise the low voltage state means the light source has not been detected.

光源探测是利用光敏电阻阻值分压的变化向单片机输入，电压大于某值时，单片机认为是高电平，即表示该路探测器感应到光源，否则为低电平，即表示探测器没有感应到光源，如此即可由两路信号判断光源的方向。

At that time, Western occupation of the three countries in the region were originally issued by the monetary integration was the action, but ruled out in the occupied territories of the Soviet Union, the so-called issue of the deutsche mark, and in the occupied territories of the Soviet Union also issued a short span of three days after the East Marker, has become a symbol of East and West Germany formally separated.

当时西方三国占领区内原本分别发行的货币进行了整合的动作，但却排除苏联占领区，发行了所谓的西德马克，而苏联占领区也在短短三日后发行了东德马克，俨然象征东西德正式分离。

FMD of the brachial artery was examined by high-resolution ultrasonography technique and the inter-ventricular septum thickness, left ventricular posterior wall thicknessand left ventricular diastolic diameter were measured by echo-cardiography technique. The left ventricular mass and LVMI were calculated according to Devereux formulary. According to Framingham heart study, The hypertensive individuals were separated into two groups, left ventricular hypertrophy group and without left ventricular hypertrophy group.

利用高分辨超声技术测定肱动脉血流介导的舒张功能；超声心动图评价受试者的心脏结构功能，测量室间隔厚度(inter-ventricular septum thickness， IVST)、左室后壁厚度(left ventricular posterior wall thickness， LVPWT)及左室舒张末内径(left ventricular diastolic diameter， LVDD)，根据Devereux公式计算(left ventricular mass， LVM)按体表面积得出LVMI，并据Framingham心脏研究的标准将高血压组分为左室肥厚组(24例)与无左室肥厚组(31例)。

Thress homologous chromosomes of trisomics associated as a trivalent or a bivalent and a univalent or three univalent during diakinesis and metaphase 1 ;Laggard chromosome and univalent separated in advance at anaphase I ,leading to distribution of 10-1-10,9-12,11-11,10-2-10,10-12,10-10,9-1-11 besides 10-ll;And there was distribution of 11-11-10-10, 10-10-10-12, 10-10-10-11, 10-11-11-11, 11-11-10-9, 10-10-10-10, 9-12-10-10, 9-9-11-11, 11-10-10-9, 12-10-9-11, 11-9-9-13, 10-10-10-9 at anaphase II ;n l microspore frequency were different of four trisomics.

三体在终变期和中期Ⅰ，3个同源染色体联会成三价体，或2个联会成二价体和1个单价体，或3个都为单价体；后期Ⅰ出现落后染色体或单价体提前分离，导致除10-11式分离外，还有10-1-10，9-12，11-11，10-2-10，10-12，10-10，9-1-11等不均衡分离方式；后期Ⅱ有11-11-10-10，10-10-10-12，10-10-10-11，10-11-11-11，11-11-10-9，10-10-10-10，9-12-10-10，9-9-11-11，11-10-10-9，12-10-9-11，11-9-9-13，10-10-10-9等分离方式；4个三体中的n 1小孢子频率不同。

From the viewpoint of the dialectical materialism,form and content are not separated.

按照辩证唯物论的观点，形式和内容是不可分离的。

Foods high in this vitamin include oily fish such as salmon, tuna and, and fish liver oils.

维生素D含量高的食物包括诸如大麻哈鱼、金枪鱼和鲭鱼之类的油鱼以及鱼肝油。

We need you to cosign our public letter demanding that press intimidation cease immediately, and that all charges be dropped.

我们需要您cosign我们的公共信，要求记者立即停止恐吓，并要求所有收费下降。