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In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系,给植物种质资源的创建提供理论依据,试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物,运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列,经比较分析,结果表明:这些同源序列的相似性达80%以上,所推导的氨基酸序列相似性达70%以上,且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%,属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%;由氨基酸序列构建的分子系统树可知,在亲缘进化关系上芸薹属与萝卜属较近,其他依次为蔊菜属、拟南芥属、荠菜属,而与诸葛菜属最远。

Results indicated that the most appropriate air temperature and earth temperature of budding was 13~14℃, 11~12℃ respectively; stem emerging was 15~16℃, 12~14℃ respectively; conidial production was 26~27℃, 20~22℃ respectively.

采用塑料大棚、小拱棚、露地栽培3种种植模式,研究了不同种植模式对蕨菜生长环境条件、生长发育和产量的影响。

Results showed that in the water body of Xizi Lake, annual average of culturable planktonic ammonifiers and nitrogen fixers were 510 and 236 CFU/mL, respectively; Ammonia oxidizers, nitrite oxidizers, nitrate reducers and denitrifiers were 8.5, 16, 587 and 16 MPN/mL, respectively; Inorganic phosphate solubilizing bacteria (1PB) and organic phosphorus mineralizing bacteria were 89 CFU/mL and 37 MPN/mL, aerobic and anaerobic cellulose decomposers were 7 and 5 MPN/mL, respectively.

水体中可培养异养细菌和固氮菌的年平均值分别为510和236CFU/mL,氨氧化细菌、亚硝酸氧化细菌、硝酸盐还原菌和脱氮菌的数量分别为8.5、16、587和16 MPN/mL;无机磷和有机磷分解菌分别为89 CFU/mL和37 MPN/mL;好氧性纤维素分解菌和厌氧性纤维素分解菌只有7和5 MPN/mL。

The inhibition level by daphnetin for 1, 2, 3 and 4 h were 7%, 51%, 69% and 75% respectively, while the values treated by daphnetin-Fe complex were 8%, 6%, 11% and 9% respectively. The inhibition level by daphnetin at different concentrations (0.1, 1, 100 and 1 mmol/L) for 6 h was 3%, 31%, 58% and 93% respectively and while the values treated by daphnetin-Fe complex were 8%, 6%, 11% and 9%.

经瑞香素(100 μmol/L)作用1、2、3和4 h后RNR活性分别被抑制7%、51%、69%和75%;在瑞香素浓度为0.1、1、100和1 000 μmol/L,作用6 h后,RNR活性分别被抑制3%、31%、58%和93%;而瑞香素-Fe复合物作用6 h后RNR活性分别被抑制8%、6%、11%和9%。

Experimental results show that the water velocity is that when the BV ammonia concentration of 50 mg, L - 1 simulation wastewater and 98 mg, L - 1 point of industrial wastewater, penetrate respectively C/C0 = 0.3 and 0.15, water column of natural zeolite could handle respectively; 126 BV, 43 BV Modified zeolite column can handle respectively 157 BV, water, and with 46 BV hydraulic load increases, zeolite column can reduce water treatment, The experiment was found to leak after 8 NaCl solution with zeolite desorption regeneration effect is good, low cost, regeneration of zeolite adsorption capacity decrescent, Test through dynamics analysis found that zeolite column of ammonia nitrogen adsorption fast.

实验结果表明,进水速度为2.72 BV时,通入氨氮浓度为50 mg·L-1的模拟废水和98 mg·L-1的工业废水,此时穿透点分别为C/C0=0.3和0.15时,得到天然沸石柱可处理水量分别为126 BV、43 BV;改性后沸石柱可处理水量分别为157 BV、46 BV,且随水力负荷增大,沸石柱可处理水量减小;试验还发现对泄露后沸石用8%NaCl溶液解吸再生效果好,成本低,再生后的沸石吸附容量变小;试验通过动力学分析发现沸石柱吸附氨氮速度较快。

The sludge settling characteristic is satisfying with SV varying from 22 to 30 and SVI varying from 58 to 80ml/g.The sludge bulking problem has been avoided successfully. The excess sludge can be thickened to 3%-4 %. The alkalinity remains equilibrious between the nitrification and denitrification in SBR-BAF system. The COD and TP loading of SBR are 2.08kgCOD/kgMLSS·d and 41.68gTP/kgMLSS·d, respectively. The NH4+-N loading of BAF is 1.16kgNH_4~+-N/m~3.d. The total HRT is about 10 hours which is only about half of suspended growth process for simultaneous phosphorous and nitrogen removal. Under the above mentioned high loadings and short HRT, the mean removal efficiency of COD, TP, NH4+-N, TN is 96%, 98%, 93%, and 84%, respectively. The mean effluent concentration of COD, TP, NH4+-N, TN, SS is 20mg/L, 0.23 mg/L, 3.24 mg/L, 7.68 mg/L, 5 mg/L respectively, which means every index can meet the first class of Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant.

最后,以上述最佳运行参数控制SBR-BAF系统运行,长期运行结果表明:系统中悬浮污泥的絮凝、沉降性能良好,SV值为22~30,SVI值为58~80,不再有污泥膨胀之虞,而且可以对剩余污泥进行重力浓缩,浓缩后污泥浓度可以达到3~4%;短程硝化和反硝化使得系统可以维持碱度平衡,无需额外补充碱度;系统在高负荷(SBR反应器COD负荷为2.08kgCOD/kgMLSS.d,TP负荷为41.68gTP/kgMLSS.d,BAF反应器NH4+-N负荷为1.16kgNH_4~+-N/m~3.d)、低水力停留时间(SBR反应器9h,BAF反应器1h,总计10h,约为同步脱氮除磷悬浮污泥工艺的一半)下稳定高效运行,对COD(不计BAF加入的外碳源)、TP、NH_4~+-N、TN的平均去除率为96%、98%、93%、84%,出水COD、TP、NH4+-N、TN、SS的平均浓度为20mg/L、0.23 mg/L、3.24 mg/L、7.68 mg/L、5 mg/L,各项水质指标均达到《城镇污水处理厂污染物排放标准》(GB 18918-2002 )一级标准的A标准的要求。

Results The positive percentage in superficial gastritis, deep gastritis, gastritic ulcer, duodenal ulcer, chronic atrophic gastritis and gastric cancer, were 70.3, 72.2, 77.7, 69.2 and 25 respectively by URT, 59.2, 77.8, 71.4, 70.7, 75 and 78 respectively by PCR, and 48.1, 61.1, 70.3, 70.7, 66.7, 66.6 and 72.2 respectively by cagA gene probe detection. Conclusions Statistics suggest that cagA+ H.

结果 浅表性胃炎、深度胃炎、胃溃疡、十二指肠溃疡、慢性萎缩性胃炎及胃癌病人的检测结果是:尿酶检测阳性百分率为:70.3、72.2、77.7、69.2、25;PCR阳性百分率分别为:59.2、77.8、71.4、70.7、75、78;基因探针杂交检测阳性率分别为:48.1、61.1、70.3、70.7、66.7、66.6、72.2。

Results The positive percentage in superficial gastritis, deep gastritis, gastritic ulcer, duodenal ulcer, chronic atrophic gastritis and gastric cancer, were 70.3, 72.2, 77.7, 69.2 and 25 respectively by URT, 59.2, 77.8, 71.4, 70.7, 75 and 78 respectively by PCR, and 48.1, 61.1, 70.3, 70.7, 66.7, 66.6 and 72.2 respectively by cagA gene probe detection.

但Hp 偏方感染人群大多无症状,只有少数人会表现不同程度的症状,Hp存在不同毒力的菌株〔4,5〕是主要原因爱情之一。cagA+株被大多数学者认为是强毒株,与消化性糖尿病溃疡、萎缩性胃炎、胃癌密切相关〔2,4〕。

Results The positive percentage in superficial gastritis, deep gastritis, gastritic ulcer, duodenal ulcer, chronic atrophic gastritis and gastric cancer, were 70.3, 72.2, 77.7, 69.2 and 25 respectively by URT, 59.2, 77.8, 71.4, 70.7, 75 and 78 respectively by PCR, and 48.1, 61.1, 70.3, 70.7, 66.7, 66.6 and 72.2 respectively by cagA gene probe detection.

但Hp感染人群大多无症状,只有少数人会表现不同程度的症状,Hp存在不同毒力的菌株〔4,5〕是主要原因之一。cagA+株被大多数学者认为是强毒株,与消化性溃疡、萎缩性胃炎、胃癌密切相关〔2,4〕。

The detection rates of methicillin-resistant S. aureus and penicillin-nonsusceptible S. pneumoniae were 15.9% and 84.3%, respectively. The prevalence of ESBLs-producing strains in Klebsiella pneumoniae, Escherichia coli, Serratia spp and Enterobacter cloacae were 31.2%, 46.2%, 94.8% and 16.8% respectively. The resistance rates of Hemophilus influenzae and Hemophilus parainfluenzae to ampicilin were 40% and 36% respectively.

耐甲氧西林金黄色葡萄球菌检出率为15.9%;对青霉素不敏感的肺炎链球菌(包括PISP和PRSP)检出率为84.3%;肺炎克雷伯菌、大肠埃希菌、粘质沙雷菌和阴沟肠杆菌产ESBLs的检出率分别为31.2%、46.2%、94.8%和16.8%;流感嗜血杆菌和副流感嗜血杆菌对氨苄西林的耐药率为36%和40%;铜绿假单胞菌和鲍曼复合不动杆菌对亚胺培南的耐药率分别为10.7%和13.2%。

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After oxidation, pH value of the surface of carbon black has a specific relation with the volatile content.

氧化后炭黑表面的pH值与挥发分之间有一定的关系。

Knowing that such a person is warped and sinful; he is self-condemned.

因为知道这等人已经背道,犯了罪,自己明知不是,还是去做。

My hobbies are travel and stamp-collecting.

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