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Contents of salidroside and tyrosol in Rhodiola roots were determined. Salidroside content was 2.147%, the highest in Rh. sacra from Tibet, then in Rh. crenulata and Rh. tibetica from Tibet, 1.763% and 1.271%, respectively; tyrosol content was 0.2985%, the highest in Rh. crenulata from Sichuan, then in Rh. tibetica and Rh. crenulata from Tibet, 0.2143% and 0.1836%, respectively; whereas both contents were very low in Rh. jistigiata and Rh. kirilowii from Sichuan with salidroside contents of only 0.034% and 0.003%, and tyrosol contents of 0.0164% and 0.0048%,respectively.

分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量,西藏产圣地红景天中红景天苷含量最高,达到2.147%,其次为藏产大花红景天和西藏红景天,分别为1.763%和1.271%;四川产大花红景天中酪醇含量最高,为0.2985%,其次是藏产西藏红景天和大花红景天,分别为0.2143%和0.1836%;长鞭红景天和狭叶红景天中2种有效成分的含量均很低,红景天苷含量仅为0.034%和0.003%,酪醇含量则分别为0.0164%和0.0048%。

Firstly, normal mice was given orally by SM(qd×2d), and the dysbacteriosis were caused in them ,and then they were given orally by STM,these mice were formed experimental salmonellosis models ,finally these salmonellosis models were treated by bifidobacterium ,and count respectively the cfu/g of STM number in the mice excrement during different time of therapy,detect the index numbers of liver and spleen respectively, detect content of TNF-a in blood and in spleen respectively.

先用硫酸链霉素给正常小鼠灌胃2d,出现菌群失调症状,再用STM灌胃造成小鼠STM感染,使用两岐双岐杆菌液干预治疗STM感染小鼠,检测不同治疗时间各组小鼠粪便STM菌落数、肝脾指数、血及脾中TNF-a的含量。采用SPSS12.0统计软件包进行数据处理分析,统计学方法采用双因素重复测量资料的方差分析、单因素随机化设计的方差分析、卡方检验、多组均数比较的非参数秩和检验。结果:随着治疗天数的增加,STM菌落数逐渐减少,未干预组、生理盐水组及双歧杆菌组比较结果均为P<0.01;组间比较,双歧杆菌组STM数下降最快。

The average recoveries were 60.2%~125% and 64.5%~126% for the drugs in plasma and urine except those of perphenazine, thioridazine and chloropromazine in plasma were 37.6%~57.5%, 36.3%~48.3%, 52.4%~67.4%, respectively; trazodone and diazepam in urine were 100%~142% and 108%~177%, respectively. The relative standard deviations of all the drugs in plasma and urine were within 0.8%~26% and 2.6%~18%(n=6), respectively. The detection limits of the 4 barbitals ranged from 20 to 100 mg/L and those of the other drugs ranged from 0.05 to 2.0 mg/L.

血浆样品中待测组分的加标回收率除了奋乃静、硫利哒嗪和氯丙嗪的分别为37.6%~57.5%, 36.3%~48.3%和52.4%~67.4%外,尿液样品中待测组分的加标回收率除了曲唑酮和地西泮的分别为100%~142%和108%~177%外,血浆和尿液中其余待测组分的加标回收率分别为60.2%~125%和64.5%~126%,相对标准偏差分别为0.8%~26%和2.6%~18%(n=6);除了巴比妥类药物的检出限为20~100 mg/L外,其余药物的检出限均为0.05~2.0 mg/L。

Results There were 1230 and 1038 cases in 2004 and 2005 respectively. The cases under 15 years old accounted for 96.58%(1188/1230) and 97.69%(1014/1038) respectively. The ratio of male to female was 1.58∶1 and 1.57∶1, respectively.

结果 2004、2005年分别报告乙脑病例1230和1038例,15岁以下病例分别占总病例数的96.58%(1188/1230)和97.69%(1014/1038),男性与女性病例之比分别是1.58∶1和1.57∶1;发病主要集中在7-8月,分别占78.21%(962/1230)和79.09%(821/1038)。

Results the intakes of meats milk and fruits had increased 71.2g、9.0g and 22.7g respectively compared with 1992. high quality protein was increased and accounted for 46.9% of dietary protein.energy and protein intake was 2207.2kcals and 64.4gper reference man per day, accounted for 91.8% and 85.4% of rni respectively. fat provided 31.3% of energy.retinol, riboflavine wear lower,only accounted for 30.4% and 59.1% of rni respectively. the prevalence of undernourishment was 12.9%,overweight was 4.4% and obesity was 9.1%.

结果 与1992年结果比较,广西居民畜禽肉、奶类、水果等食品摄入量分别增加了71.2g、9.0g和22.7g,优质蛋白质来源达到46.9%;每标准人口摄入的能量、蛋白质分别为9226.2kj和64.1g,分别占推荐摄入量的91.8%和85.4%;而脂肪供给的能量占31.3%,视黄醇当量和核黄素摄入偏低,仅分别达到推荐标准的30.4%、59.1%,钙摄入仅达到适应标准的39.4%。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。

The linear concentration ranges for γ-aminobutyric acid, valine and leucine were 5.0×10^(-3)-0.12 g/L, 5.0×10^(-3)-0.08 g/L and 5.0×10^(-3)-0.12 g/L, respectively, and the detection limits (S/N=3) were 2.5×10^(-3), 2.5×10^(-3) and 2.6×10^(-3) g/L, respectively. The relative standard deviations of the peak currents of γ-aminobutyric acid, valine and leucine were 2.5%, 4.9% and 3.9%(n=7), respectively.

氨基丁酸、缬氨酸和亮氨酸的线性范围分别为5.0×10^(-3)~0.12、5.0×10^(-3)~0.08和5.0×10^(-3)~0.12 g/L,检出限(S/N=3)分别为2.5×10^(-3)、2.5×10^(-3)、2.6×10^(-3)g/L;7次平行进样峰高的相对标准偏差分别为2.5%、4.9%、3.9%。

The pot experiment results showed that inoculation with A1501 and A1513 could promote the growth and grain yield of rice. Comparison with the non\|inoculation, the grain yeild of rice treated with A1501 and A1513 increased by 8 5% and 10 3% respectively. And %Ndfa of rice shoot and grain estimated by 15 N\|isotope dilution method was 9 00% and 11 5%, respectively, which was consistent with the increment of the total N(8 5% and 11 6%,respectively).

盆栽结果表明,接种A.faecalis 显著提高了水稻的生长,接种A1501 和A1513 的水稻稻谷产量分别比未接种提高了85%和103%,用15N 同位素稀释法估测水稻地上部总氮中来源联合固氮的百分率达900%和115%,其结果与水稻地上部总氮量的增加幅度(86%和116%)基本一致。

And Citrobacter sp., respectively. Both of them were γ-proteobacteria. 3 IPB strains closely matched to Bacillus megaterium, Terrabacter tumescen and Janthinobacteriwn lividum. respectively, and 6 OPB strains were Azospirillum sp., Enterobacter aerogenes. Blastobacter aggregates, Staphylococcus epidermidis, Acinetobacter haemolyticus and Cryptococcus sp., respectively.

对3株有代表性的无机磷分解菌和6株占优势的有机磷分解菌的鉴定显示,3株无机磷分解菌与巨大芽孢杆菌、大地杆菌和Janthinobacterium lividum最相近,而6株有机磷分解菌分别为固氮螺菌,产气肠杆菌,Blastobacter aggregatus,表皮葡萄球菌,溶血不动杆菌和念珠菌。

After antituberculotic treatment for 6 months.The rates of sputum coversion of group 1,group 2 and group 3 were 76.92%,94.79% and 100% respectively.The mean sputum coversion time of group 1,group 2 and group 3 were 12.46 weeks 5.0 weeks and 3.0 weeks respectively and the rates of focus absorption of group 1 ,group 2 and group 3 were 61.54%. 86.46% and 100% respectively.

经过6个月抗痨治疗后,Ⅰ组痰菌转阴率为76.92%,平均转阴时间为12.46周,病人疗程结束时,肺内病灶X线吸收占61.54%;Ⅱ组痰菌转阴率为94.79%,平均转阴时间为5.0周,病人疗程结束时,肺内病灶X线吸收占86.46%;Ⅲ组痰菌转阴率为100%,平均转阴时间为3.0周,病人疗程结束时,肺内病灶X线吸收占100%。

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It is also known as one of the most poisonous naturally occurring substances.

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The greatest stress is found at the location on the cross section where V is the largest.

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