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Accordingly, the Q bands of them blue shifted by 6mn and 13nm, respectively. The fluorescence emission wavelengths of Cd-pheophytin a and Cd-pheophytin b observed at 660nm and 649nm, respectively, which were shorter than those of corresponding pheophytin a and pheophytin b by 10nm and 9nm, respectively.

研究发现:在光吸收性质上,与脱镁叶绿素a和b相比,镉叶绿素a和b在Soret带的吸收峰位置分别红移了19nm和11nm,而在Q带则分别蓝移了6nm和13nm;在荧光性质上,镉叶绿素a和b的荧光发射波长比脱镁叶绿素a和b分别蓝移了10nm和9nm。

Results The linear ranges of tanshinol sodium and protocatechualdehyde were 42.52~382.68μg/mL and 8.14~ 73.30μg/mL, respectively; the correlation coefficients were 0.999 8 and 0.999 6, respectively; the average recoveries of spiked sample were 100.54%(RSD=0.797%) and 98.87%(RSD=1.282%), respectively.

结果丹参素钠在42.52~382.68μg/mL浓度范围内线性关系良好(r=0.999 8),平均回收率为100.54%(RSD=0.797%);原儿茶醛在8.14~73.30μg/mL浓度范围内线性关系良好(r=0.999 6);平均回收率为98.87%(RSD=1.282%);结论此法简便,快速,准确。

Results:There were 30 patients with systemic sclerosis (42.2%),10 with Sjogren′s syndrome(14.1%),6 with systemic lupus erythematosus (8.5%),5 with rheumatoid arthritis and localized sclerosis (7.0%) respectively,4 with only Raynaud′s phenomenon without any definite diagnosis (5.6%),2 with CREST syndrome,overlap syndrome,cirrhosis of unknown causes and buerger′s disease (2.8%) respectively,and 1 with primary billiary cirrhosis,sclerous cholangitis,and chloasma-vitiligo (1.4%) respectively.

结果:ACA可在多种疾病中出现,其中系统性硬化症30例(42.2%),干燥综合征10例(14.1%),系统性红斑狼疮6例(8.5%),类风湿关节炎5例(7%),局限型硬皮病5例(7%),仅有雷诺现象但诊断不清者4例(5.6%),CREST综合征、重叠综合征、不明原因肝硬化、闭塞性脉管炎各2例(2.8%),原发性胆汁性肝硬化、硬化性胆管炎、黄褐斑合并白癜风各1例(1.4%)。

Result: The absorption rates in rat stomachs at 2 h after administration was 76.31%; The absorption rates at colon, duodenum, ileum and jejunum were 46.25%, 40.54%, 38.21%, 32.77%, respectively. The absorption rate constant at concentrations of 10.014 4, 20.028 8-40.057 6 mg·L-1 in intestine were 0.643 4, 0.613 7, 0.597 0 h-1, respectively. The K a of scopoletin at pH of 6.0, 6.8 and 7.4 in intestine were 0.621 7, 0.603 3, 0.613 7 h-1, respectively.

结果:东莨菪素在大鼠胃中2 h的吸收百分率为76.31%;东莨菪素在结肠、十二指肠、回肠、空肠的吸收率分别为46.25%,40.54%,38.21%,32.77%;不同质量浓度东莨菪素10.014 4,20.028 8,40.057 6 mg·L-1在小肠的吸收速率常数分别为0.643 4,0.613 7,0.597 0 h-1;不同pH(6.0,6.8,7.4在小肠的吸收速率常数分别为0.621 7,0.603 3,

The sum of detecting stenosed or occluded VA by CDI, TCD and CDI plus TCD confirmed by DSA were 60, 59 and 67 arteries respectively. The SEN was 87.1%, 83.8% and 100%,SPE was 89.2%, 89.1% and 97.3% respectively. The OA was 88.2%, 86.8% and 98.5% respectively.

结果 DSA提示VA狭窄或闭塞为31支,阴性37支,CDFI、TCD和综合结果与DSA符合分别为60、59和67支,敏感性分别为87.1%、83.8%和100%,特异性分别为89.2%、89.1%和97.3%,准确率分别为88.2%、86.8%和98.5%。

A single dose of albendazole 400 mg was used as control. Results The egg negative conversion rates of ivermectin and albendazole were both 100%(34/34) for Ascaris infection, 17.6%(6/34)and 76.5%(26/34)respectively for hookworm infection, 67.6%(23/34)and 47.1 %(16/34)respectively for Trichuris infection,58.8%(20/34) and 100%(34/34)respectively for Enterobius infection.

结果随机双盲治疗,伊维菌素和阿苯达唑对蛔虫感染的虫卵阴转率均为100%(34/34),对钩虫感染的虫卵阴转率分别为17.6%(6/34)和76.5%(26/34),对鞭虫感染的虫卵阴转率分别为67.6%(23/34)和47.1%(16/34),对蛲虫感染的虫卵阴转率分别为58.8%(20/34)和100%(34/34)。

Flow cytometric analysis showed that after mock infection, Ad5null infection, and Ad5hSeFT infection, the mean fluorescence intensity values for the binding of Ulex europaeus Ⅰ lectin to NIH3T3 cells were 2. 3±0.6, 2. 1±1. 0, and 36. 5±5. 9, respectively; MFI values for the binding of Griffonia simplicifolia isolectin B4 (GS-IB4) lectin to NIH3T3 cells were 167±23, 170±19, and 100±14, respectively; MFI values for the binding of human natural IgG and IgM antibodies to NIH3T3 cells were 31±3, 32±4, and 22 ±4, respectively.

流式细胞分析结果表明,小鼠NIH3T3细胞及分别被Ad5null和Ad5hSeFT转导的NIH3T3细胞,UEA-1凝集素结合细胞的平均荧光强度值分别为2.3±0.6、2.1±1.0和36.5±5.9;GS-〓凝集素结合细胞的MIF值分别为167±23、170±19和100±14:人天然IgG和IgM抗体结合细胞的MFI值分别为31±3、32±4和22±4。

In clean diluent condition, the median lethal concentration (MLC50) of CIO2 against bacteria and yeast was 9.4 and 8.4 ppm, respectively. In dirty diluent condition, the MLC50 of CIO2 against bacteria and yeasts was 39.9 and 24.3 ppm, respectively. The virucidal activity of CIO2 against 100 median embryo lethal dose (100 ELD50) of Newcastle disease virus, avian influenza virus, infectious bronchitis virus and infectious bursal disease virus was 1.2, 1.2, 2.5 and 1.2 ppm, respectively.

发现CIO2在乾净稀释液中对细菌和酵母菌的中间致死浓度(MLC50)分别为9.4和8.4ppm,CIO2在污浊稀释液中对细菌和酵母菌的MLC50分别为39.9和24.3ppm,CIO2之杀灭新城病病毒、H6N1型家禽流行性感冒病毒、传染性支气管炎病毒和传染性华氏囊炎病毒的浓度分别为1.2、1.2、2.5和1.2ppm。

Results The sensitivity of Cox-2 protein、Survivin protein and urinary cytology was 88.3%、68.3%、21.7% respectively, the specificity was 93.3%、80.0%、100% respectively,and the Youden index was 81.6%、48.3%、21.7% respectively.

结果 尿Cox-2蛋白、尿Survivin 蛋白和尿脱落细胞学的敏感性分别为88.3%,68.3%,21.7%;特异性分别为93.3%、80.0%和100%;Youden指数分别为81.6%、48.3%和21.7%。

In this study, genetic analyses were conducted to determine the genetic basis in an elite resistant inbred line Siyi with complete resistance to maize dwarf mosaic. A new genetic model, two dominant complementary genes conditioning the resistance, were found by Mendelian genetic analysis based on parents, F1, F2 and backcrosses in three successive years' field trails. The two genes were further mapped near the centromere of chromosome 3 and 6, respectively by tightly linked microsatellite markers using 242 plants from F2 generation. The resistance gene on chromosome 3 is 1.0 cM apart from the flanking markers phi053 and umc1527, respectively. Whereas the linkage distance between two flanking markers bnlg1600 and phi075 and resistance gene on chromosome 6 was 1.0 and 4.0 cM, respectively. Genotypic analysis of the plants from testcross and F3 populations supported the new genetic manners.

课题组通过连续三年的抗病鉴定,在国内种质资源中筛选出一份综合农艺性状优良、配合力较高的自交系四一,三年的表型遗传研究和两年的分子标记工作,发现四一中的玉米矮花叶病抗性是由两对显性互补基因控制的,进而利用F2作图群体,把发现的两个基因定位在第三和第六染色体的着丝点附近,并获得了双侧紧密连锁的分子标记连锁图谱,其中第三染色体上的分子标记UMC1527和phi053从抗病基因双侧逼近1 cM,而第六染色体上的分子标记phi075、bnlg1600从抗病基因双侧分别逼近4 cM和1 cM;利用B2群体、F2:3家系、BC3F1群体和带有第三、第六染色体抗病基因以及两个抗病基因的近等基因系,进一步证实了四一中成株期抗性是由两个显性互补基因控制的。

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