查询词典 insert cells

It is very similar between the secretion of rat pancreatic β cells and gonadotropes in general. Their secretions is also very similar to those observed in other endocrine cells, such as adrenal chromaffin cells. This study further proved that the three phasic process of secretion are ubiquitous.

虽然具体的分泌特性有所不同，但大鼠胰腺β细胞与垂体促性腺激素细胞的分泌情况在总体上很相似，这与前人在其他细胞上的发现也非常相似，本文的实验进一步证实了这种三个阶段的分泌过程和机制在不同的细胞中是普遍存在的。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

After propagation, GTH cells evacuated large number of secretory granules, TSH cells appeared vacuoles, rough endoplasmic reticulum in STH cells were circumnuclear.

繁殖后，GTH细胞中的分泌颗粒出现排空；TSH细胞有空泡；STH细胞中的粗面内质网呈现围核环状排列。

Xenopus laevis Tadpole (1) Xenopus laevis Tadpole was capable of lens regeneration, which originated from the outer cornea.(2)The days of lens regeneration of Xenopus laevis Tadpole was earlier than Bufo Raddei Strauch Tadpole, The rate of regeneration of Xenopus laevis Tadpole was higher than Bufo Raddei Strauch Tadpole.(3)During Xenopus laevis lens regeneration the outer cornea cells proliferated, in the elongating cells the mitochondria became complicated and in the columnar cells the cisternal dilating of endoplasmic reticulum was ubiquity.

非洲爪蟾蝌蚪(1)再生晶状体来源于外角膜；(2)晶状体再生较花背蟾蜍蝌蚪晶状体再生时期早，再生率高；(3)电镜观察发现再生前期外角膜增殖，观察到晶状体上皮细胞和伸长细胞(进一步发育为晶状体纤维细胞)，伸长细胞中线粒体形状各异随着进一步的分化，细胞器逐渐消失；分化过程中晶状体上皮细胞粗面内质网增多，并且内质网扩张普遍存在。

The larvae should be fed Isochrysis zhanjianggensis (5.0×l0^4 cells/mL), supplemented with Nitzschia closterium (3.0×10^4 cells/mL), and Chlorella sp.(10.0×10^4 cells/mL).

4幼虫培育期间的饵料以湛江等边金藻为主（5.0万个／mL）并辅以新月菱形藻（3.0万个／mL）、扁藻（0.4万个／mL）、小球藻（10.0万个／mL）。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Methods Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm×3 mm×4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with α1cDNA probe.

采取成年雄性SD大鼠肋软骨细胞，以3 mm×3 mm×4 mm大小的块状多孔HA为载体培养软骨细胞；培养3天和7天后通过扫描电镜观察HA表面和中矢横断面孔隙中有无细胞生长，同时将培养2天的细胞爬片以α1cDNA片段为探针进行原位杂交，求证培养的细胞是否软骨细胞。

The results showed that the surface and cryptal lining cells of the pseudostratified ciliated columnar epithelium and the superficial squamous cells of the metaplastic squamous epithelium both underwent membranous expression of Fas, but the basal cells did not.

为了探讨Fas在鼻咽癌癌细胞凋亡过程中的作用，我们对37例未经治疗的非角化性人体鼻咽癌组织癌细胞Fas表达进行了研究。

At 24 hours after the removal of 5-FU, most of the epithelial cells were increased in number, showing cuboidal and merged into pieces. At 48 hours, only few positive cells could be seen with some of the pseudostratified mucociliary epithelium, with ciliated cells.

氟尿嘧啶去除6h后，气管基底膜上可见少量扁平样细胞。24h后基底膜上细胞数目增加，并逐渐分化呈立方状，连接成片。48h后气管上皮中出现假复层柱状上皮，并可见纤毛细胞。

Tom: You are right, he worked hard all the time.

你是对的。他一直都是努力工作。

Take shortcomings,failures,and mockings of others as commonplace and a drive to complete oneself,hold your own opinion over the evalution and disscution divested from others,afterwards sink into the state of eliminate mistakes if any or encourge oneself being not being influenced by the sayinngs of others or having no ideas to do.

把缺点、失败及别人的耻笑等看成是一种常事，当成完善自己的动力，对别人的评价和议论自己心中有主见，做到&有则改之，无则加勉&，不为人言所左右或无所适从。