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Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

Flow cytometry results showed that: 1） When cells were treated with Diallyl trisulfide at the concentration of 10 μg/mL for 12 h and 24 h the percentage of G0/G1 phase cells was decreased and that of G2/M phase cells was significantly increased compared with those in the control group.2） Diallyl trisulfide down regulated Bcl expression while up-regulated bax expression.

结果：MTT显示大蒜素能抑制人结肠癌 HT29细胞的增殖，并具有时间-剂量依赖性；流式细胞术测定结果显示10 μg/ml大蒜素可明显诱导HT29细胞凋亡，并将细胞周期阻滞在G2/M期；可降低癌基因蛋白Bcl表达，而促进Bax表达。

Cells cultured with method 2 became smaller after 2 hours, formed dipolar or multipolar body cells, and most of cells were died after 48 hours.

方法2诱导2 h即可见细胞体积变小，形成双极或多极的细胞体，可持续诱导48 h，后大部分细胞浮起死亡。

Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.

结果： 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达：呈核周散在分布的棕黄色颗粒，5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒，个别细胞间可见此结构，类似正常心肌的闰盘结构。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

In PsNPV infected treatment, the color of the gland was purple-black dyed with eosin and the cells were distortedly squeezed together. The tracheae nearby showed grievous pathological changes and a lot of white granules deposited around it. The pathological changes in PsGV infected treatment showed another pattern. The gland body was relative small with smaller individual cells, most of which were red dyed with eosin. The cell limits were obscure.In coinfected treatment,there were few gland cells colorated by esoin.

结果表明，不同感染组粘虫前胸腺腺体都有不同程度的组织病变，PsNPV感染组在感染晚期与前胸腺相连的气管严重病变，出现大量白色颗粒状物累积，被伊红染成紫黑色，腺体细胞被挤压变形；PsGV感染组的前胸腺腺体变小，单个细胞也小，细胞界限不十分明显；两种病毒混合感染组的腺体细胞小，能被伊红染色的细胞极少。

We theoretically investigated the effects of incorporating Ih, T-type Ca2+ current, sustained inward current, and/or low-voltage-activated L-type Ca2+ channel current on 1 creation of BP cells, 2 robustness of BP activity to electrotonic loads of nonpacemaking cells, and 3 BP cell ability to drive NP cells.

我们从理论上研究了合并Ih， T-型 Ca2+流，持续内向电流，和/或低电压激活的L-型Ca2+通道电流对1产生 BP细胞， 2 BP 活性对非起搏细胞电紧张负荷的稳定性，和 3 BP 细胞驱动 NP细胞的能力的作用。

To understand global features of gene expression changes during in itro neural differentiation, we carried out the microarray analysis of embryonic stem cells, embryonal carcinoma cells, and adult neural stem/progenitor cells.

中文摘要：为了了解在体外神经分化期间基因表达改变的全面特征，我们对胚胎干细胞，胚胎性癌细胞和成年的神经干/祖细胞进行了微阵列分析。

Scientists have known that neural stem cells play a key role in both embryonic and postnatal mice, driving the development of specialized cells within the brain such as neurons, astrocytes, oligodendrocytes, and ependymal cells.

科学家们已经知道神经干细胞在胚胎和出生后小鼠均扮演着重要角色，推动着大脑具体细胞的发育例如神经元、形细胞，胶质细胞和室管膜细胞。

Scientists hae known that neural stem cells play a key role in both embryonic and postnatal mice, driing the deelopment of specialized cells within the brain such as neurons, astrocytes, oligodendrocytes, and ependymal cells.

科学家们已经知道神经干细胞在胚胎和出生后小鼠均扮演着重要角色，推动着大脑具体细胞的发育例如神经元、星形细胞，胶质细胞和室管膜细胞。

What was it that made you want to change sider ?

是什么使你临阵倒戈？

I don't know what he wants, but let's play along with him.

我不知道他要干什么，可我