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The isotypes of the two monoclonal antibodies were identified as IgG1, and their directed epitope located in PreS1 (34-47) identified by Western blotting analysis.

并利用上述8个序列重叠的PreS1融合蛋白，通过免疫印迹的方法鉴定了它们的作用表位位于PreS1（34-47）内。

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

In the contaminated lard sample, 6 organic compounds have been thus identified to be the raw materials or byproducts in the production of organic tin compounds. Based on the identified contaminants, it can be postulated that the chemical structure of the organic tin compound is dibutyltin-bis-(2-isooctyl thioacetate). The method is simple and precise, and has not been reported in the local literature.Keywords Organic tin compound, Lard, SPME－GC－MS

经过摸索研究，采用固相微萃取方法富集挥发性杂质，直接注入色谱中，经色谱分离，质谱测定，确定了杂质的结构，由杂质成分推断生产有机锡所用的原料，然后判断合成路线，最后确定有机锡的结构，从有毒猪油中鉴定出2－巯基醋酸异辛酯、双（2－醋酸异辛酯）二硫、双醋酸异辛酯硫醚等6种与有机锡有关的杂质，并与相应的有机锡工业品对照，发现工业品中也含有这些杂质，与其合成过程的原料及副产物相符，从而确定有机锡化合物为二硫代醋酸异辛酯二丁基锡。

In order to investigate the molecular genetic basis of grain qulity and find useful information for genetic improvement to grain quality, molecular maker linkage map was construct with DH population derived from "Nong xi110×Nong xi531","DH8×DH40"and"DH86×Shen 137"by SSR markers;then 83,38 and 44 DH lines were analysised and identified for grain quality;in the end quantitative trait lociassociated traits related grain quality were identified and analyzed in this study.

本研究利用SSR标记构建基于组合&农系531×农系110&(农单5群体)、&DH8×DH40&和&DH86x沈137&的分子标记连锁图谱，并对该三个群体的83个、38个和44个DH株系的籽粒品质性状进行了分析和鉴定，以探索控制籽粒品质性状QTL位点与遗传效应，为玉米籽粒品质性状的遗传改良提供理论依据。

In application, the first coarse seismic facies classification effectively identified the channel, erevasse-splay, natural levee, alluvial flat and other subfacies etc, and the second fine reclassification aimed to the classified channel further identified the lateral bar, mid-channel bar, main channel sand body and other microfacies of a fluvial channel.

通过实际应用指出，初次的相对较粗的地震相分类研究可以有效地区分出了河道、决口扇、天然堤、河漫滩等沉积亚相，而针对刻画出的河道的再次地震相分类研究则可以有效地区分出了边滩、心滩和主河道砂体等沉积微相，并初步证实了地震相再分类研究在客观刻画地质体细节方面的优势。

Among the 36 isolated pathogenies of bacterial wilt diseases of solanacae crops, 33 strains were identified as typical strains of R. solanacearum (91.67%). The ratios of the pathogenies identified as R. solanacearum were 100.0%, 90.0% and 89.5% on eggplant, tomato and pepper, respectively.

经选择性培养分离得到的36株青枯病原菌中，有33株鉴定为典型的青枯雷尔氏菌，占91.67%；茄子、番茄和辣椒上的青枯病原菌被鉴定为青枯雷尔氏菌的比例分别为100%、90%和89.47%。

The characterestics of these strains identified from the artifically infected soft-shelled turtle are identified the primary isolate.

从感染鳖的肝脏中分离到与原分离株形态及生理生化特性相同的菌株。

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

This use of the verb can be traced back to the 18th century, but it is now somewhat less acceptable to the Panel than the first sense under discussion: 58 percent of the Panel accepts the sentence She identified herself with the campaign against drug abuse, and only 40 percent accepts She identified with the campaign against drug abuse, where no reflexive pronoun is used.

它作为动词的用法可追溯到18世纪，不过对于用法专题小组来说它没有第一种解释那样被易于接受：58%的小组成员接受她支持反对滥用药物的运动这一说法，只有40%的成员接受不要反身代词的She identified with the campaign against drug abuse 这一说法

This use of the verb can be traced back to the 18th century, but it is now somewhat less acceptable to the Panel than the first sense under discussion: 58 percent of the Panel accepts the sentence She identified herself with the campaign against drug abuse, and only 40 percent accepts She identified with the campaign against drug abuse, where no reflexive pronoun is used.

正如威尔逊·福勒特1966年写到的，&如果评论家们很早就全面放弃identifying 而重新使用 identifying himself with 伊万·卡拉玛佐夫、堂吉诃德、玛丽·波普斯等人物，那就会有助于探询出这些词的真实含意。&不过，近年来使用identify with 中间不加反身代词已成为一种标准搭配。

When I started coding, I had problems with the ASF file writer.

当我开始编码，我曾与ASF文件作家的问题。

I know that in one of the schools the boys have risen up in the classroom and enlisted in a mass.

如果你们也和他们一样，是集体自愿的话，你们的老师将是多么地光荣啊。