查询词典 fragment

The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned.

摘 要：本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较，并借助DNAstar软件设计引物，扩增并进行序列分析，旨在揭示其遗传变异的规律。

While the new doctrine seems almost certainly correct,the one papyrus fragment raises the specter that another may be unearthed,showing,for instance,that it was a posthumous production of t...59.While the new doctrine seems almost certainly correct,the one papyrus fragment raises the specter that another may be unearthed,showing,for instance,that it was a posthumous production of the Danaid tetralogy which bested Sophocles,and throwing the date once more into utter confusion.

数据资料已被统计出来，或被重新统计出来，以便证明在文体学这方面，《乞援衰女》实际上确实在《波斯人》和《七雄攻忒拜》（后两者现在则变成了&原始&的剧作）之后以及在轴残片却唤起了一种幽灵似的疑虑，即另一块残片有可能再度被挖掘出来，并证明，比如说，《乞援衰女》是作者藉以击败索福克勒斯的《达那特四部曲》的一部死后出版之作，从而将创作年代再度置于极度的混乱之中。

A gene sequence of the anthropogenic adiponectin spherical fragment obtained by the invention absolutely accords with a known gene sequence, and compared with the full-length adiponectin, the hydrolyzed anthropogenic adiponectin spherical fragment has stronger biological activity, fast reaction after dosage, good bioavailability and better clinic application prospect, especially when the diabetes patient receives a recanalization treatment after a myocardial infarction, the anemically myocardial damage of the diabetes patients can be eased by the exogenetic dosage.

本发明公开了一种人源性脂联素球状片段用于治疗糖尿病缺血性心脏病药物的应用，本发明所得到的脂联素球状片段的基因序列与已知的基因序列完全相符，与全长的脂联素相比，其水解以后的脂联素球状片段有着更强的生物学活性，而且给药后反应迅速，生物利用度好，有着更好的临床应用前景，特别是对糖尿病患者在发生心肌梗死后接受血管再通治疗时，通过外源性给药，以减轻糖尿病患者缺血心肌损伤。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整的开放阅读框，编码299个氨基酸残基的多肽，分子量约35KD。

The partial nucleotide sequence of the gene fragment showed high homology with the essential spectrin gene of Caenorhabditis elegans and the erythrocyte surface antigen gene of Plasmodium falciparum , when the gene fragment was homologically analyzed in GenBank.

与GenBank中的基因进行同源性分析，其部分核苷酸序列与秀丽隐杆线虫红细胞膜内蛋白和恶性疟原虫红细胞表面抗原高度同源

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达，包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性，以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

Other New Testament apocrypha, generally considered less important, include the Gospel of the Hebrews, the Gospel of Mary Magdalene, the Infancy Gospels, the Gospel of Peter, the Unknown Berlin Gospel, the Naassene Fragment, the Secret Gospel of Mark, the Egerton Gospel, the Oxyrhynchus Gospels and the Fayyum Fragment.

其他的新约伪经，一般被认为是较为次要，包括《希伯莱福音书》、《玛利亚抹大拉福音》，《耶稣童年的福音》，《彼得福音》，还有不知名的柏林福音译本，拿撒勒厄赛派残卷，《马可秘密福音》，《 Egerton 福音》，《俄西林古的福音书》，还有法雍的残卷。

According to the alignment of Blastn and Blastx, one differentially exp ressed fragment, A7, was 97% identical in nucleotides to the 26S ribosomal RNA gene of Photinia fraseri and 91% in amino acids to cytochrome P450 monooxygenase in maize, respectively. Another differentially exp ressed fragment, A16, was 85% in nucleotides and 93% in amino acids identical to alpha-expansin 2 in Triphysaria versicolor.

核酸和氨基酸比对分析中同源性最高的是片段A7和A16.A7的核苷酸序列同源于红叶石楠Photinia fraseri 26S核糖体RNA (97%)、蛋白同源于玉米的细胞色素P450单加氧酶(91%)， A16的核苷酸(85%)及蛋白(93%)同源于直果草属 Triphysaria versicolor 的α-expansin 2基因。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。