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A 450 nucleotides fragment of the COOH-terminal of the nucleoprotein gene and 188 nucleotides fragment of the matrix were amplified by RT-PCR and subject to sequence.

用逆转录-聚合酶链反应扩增麻疹病毒分离株的核蛋白基因羧基端450个核苷酸片段和基质蛋白基因的188个核苷酸片段。

The amplified band from M131 was sequenced and then aligned with the sequence of avr-Pila gene. The result was that the sequence was 98% homologous to one fragment of avr-Pita sequence, but the integration site was not within this fragment.

将M131的扩增片段测序后得到长度为1057bp的序列,并且将所测序列和已知的avr-Pita一段序列比较发现达98%的同源,即该序列为无毒基因的某一段序列,但插入的位点并不在所扩增片段内。

The amplifiedband from M131 was sequenced and then aligned with the sequence of avr-Pila gene. The result was that the sequence was 98% homologous to one fragment of avr-Pita sequence, but the integration site was not within this fragment.

将M131的扩增片段测序后得到长度为1057bp的序列,并且将所测序列和已知的avr-Pita一段序列比较发现达98%的同源,即该序列为无毒基因的某一段序列,但插入的位点并不在所扩增片段内。

The result of sequence analysis indicated that the length of cDNA and DNA fragments of Lectin gene and the length of cDNA and DNA fragments of Pectate Lyase gene were 508 bp,575 bp,1,316 bp and 1,858 bp respectively.The DNA fragment of Lectin gene had a intron,whose length was 67 bp .The DNA fragment of Pectate Lyase gene had 3 introns,whose lengths were 90 bp,83 bp,and 370 bp respectively. All these four introns sequences followed the rule of GT-AG.

通过序列分析得知,香蕉凝集素基因cDNA片段长508 bp;香蕉凝集素基因DNA片段长575bp,有一个长为67bp的内含子;香蕉果胶裂解酶基因cDNA片段长1,316bp;香蕉果胶裂解酶基因DNA片段长1,858bp,有三个内含子区,分别长90 bp,83 bp和370bp;在总共的四个内含子序列中,分别都符合GT-AG规则。

Using TaKaRa LA PCR? in vitro Clonging Kit, a 2.3kb DNA fragment from the mutant was amplified and identified. Sequence analysis indicated that the fragment was composed of a complete proE gene and a partial proA gene.

根据枯草芽孢杆菌93151野生菌株proB基因的序列设计并合成引物,利用TaKaRa LA PCR~ in vitro Clonging Kit试剂盒,克隆突变菌株的proBA基因,得到一个约2.3kb的DNA片段,序列分析表明该片段包含一个完整的proB基因和不完整的proA基因。

The maximum run-out distance is 3200m. The three cases are studied from the Niuquangou rockslide-debris flow at the south west segment of the main seismic fault, the Chengxi rockslide at the middle segment of the main seismic fault and the Donghekou rockslide-debris flow at the north east segment of the main seismic fault.The three cases illustrates following common characteristics:(1)parent rockmass is broken under long geologic processing and weathered;(2) Rockmass was separately expanded and thrown under long-period strong ground vibration in which the vertical motion was predominant along the main seismic fault;(3)sliding mass was crashed at the exiting zone and transferred to fragment material;(4) The secondary landslide was triggered underneath the exiting zone due to the powerful crashing and shaving processes that provides more mass to debris body and cushion shape;(5) The a long run-out distance fragment flow is occurred under air layer lubrication. The air layer was pressed and turbulently flowed in the narrow-shaped valley, or laminarly flowed in the extensive flatland.

本文重点解剖了位于地震破裂带南西段的汶川映秀牛圈沟滑坡-碎屑流、位于地震破裂带中段的北川城西滑坡和位于地震破裂带北东段青川东河口滑坡-碎屑流3个典型实例,认为具有如下特征:1)岩性条件:母岩遭受长期构造动力作用,呈碎裂岩体,后期被强烈风化,岩体极为破碎;(2)抛掷效应:位于汶川地震主断裂带或附近,垂直加速度大于水平加速度,强地面运动持时长,岩体发生振胀和抛掷;3)碰撞效应:上部滑坡体发生高位剪出和高位撞击,致使岩体碎屑化;(4)铲刮效应:撞击作用导致下部山体被铲刮,形成次级滑坡,为碎屑流体提供了足够展翼和抛洒物源体积;5)气垫效应:碎屑化岩体快速抛掷导致下部沟谷空气迅速谷状圈闭和向下紊流,形成气垫效应,或者,在下部地形开阔地带压缩空气呈层流状态致使滑体凌空飞行。

Because the same sex determination gene fragment of fowl as SRY of mammal is not found, the discovered peculiar gene fragment in XhoI family will become the basic study of sex determination gene;The basis of sex differentiation is aromitic enzyme which controls sex hormone′s transformation; sex-linked inheritance and cloaca sexing will still bethe main methods of sexualization; a female can turn into a male through hormone in ducing and the changed female can turn into its genetic sexwhile a male can′t turn similarly.

家禽中虽未发现象哺乳动物SRY基因的性别决定片段,但XhoI家族中特异片段的发现可能成为性别决定基因研究的基础,性别分化的基础是控制性激素转型的芳香化酶;伴性遗传和泄殖腔鉴别法仍然是性别鉴定的主要方法;利用激素诱导只能使雌转雄而不能使雄转为雌,且转变后仍能恢复原来的遗传性别。

Protective door single damaged action by fragment and shock wave is analyzed. Protective door damaged under compound action arisen from shock wave and fragment is discussed at last.

对破片的初始速度、质量分布和空间分布进行了定量计算,分析了破片和冲击波对防护门的单一破坏作用,定性讨论了冲击波和破片对防护门的复合破坏作用。

Undesirable structure and tree species simpleness etc are main problem in existing urban forest, influence urban forest effective exert its zoological function(2) urban forest landscape indexes show: urban forest fragment increased form 1985 to 1995, and decreased form 1995 to 2007, but as a whole, Beijing urban forest fragment is degressive, especially woodland and spinney land.

2在研究时段内北京市城市森林景观指数变化明显,从1985-1995年间破碎化程度增加,而1995-2007年间下降,1985-2007年总体呈下降趋势,其中有林地和灌木林两种主要城市森林用地类型的变化剧烈;城市森林尤其是有林地用地受到人为干扰影响强烈,斑块日益简单化,主要类型城市森林间相互转化现象严重,说明北京市城市森林在研究时段内发生了很大变化。

Of the tested plants had the same fragment at all stages of PMCs and in somatic cells, but other 44% had not, among them, however, 0.76% of their anthers produced the same specific fragment only at synizesis stage, but not at other stages or in somatic cells.

在这一片段中设计特异引物再次对兰州百合DNA进行检测,56%的个体整体存在B1片段;在剩余的44%个体中得到了0.76%的花药特异片段检出率,时期为凝线期。

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推荐网络例句

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了,请告诉我,我会帮助你摆脱困境,真的,请联系我啦!

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放,就是一个这样的例子。

Well, Jerry would rattle off all the details of that movie.

那么,杰瑞会急促背诵那部电影所有细节。