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We submitted the gene fragment to GenBank and its accession number was DQ346681. The results of BLAST showed that the gene fragment has 100% identification with turkey and chicken and 96% with human, monkey, swine, horse and dog.

本研究中的鹅OBR基因片段序列与火鸡和部分鸡的OBR基因序列同源性达100%，与人、猿、猪、马、犬等哺乳类单胃动物的同源性都达到96%。

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

Result: One of 29 (3.45%) blood clots and one of 112 (0.98%) spleen tissue from field rats were positive for 88bp DNA fragment. Eight DNA extracts from 39 (20.5%) chigger mites produced the predicted DNA fragment.

结果：从送检的29份鼠血块和112份脾组织中均检出阳性1份；从39份恙螨幼虫体内检出阳性8份。

Sequence analysis showed that the amplified 16S rDNA fragment was closely related to those in the 16Sr Ⅰgroup, with a homology rate of more than 99 %. This fragment had maximum homology, upto 100 %, with those of Ash witches' broom, Epilobium phyllody, and Chinaberry witches' broom phytoplasma strain Jiangxi. Its similarity of 16S rDNA sequence is obviously lower than 97 % as compared with other phytoplasma groups.

结果表明，该片段与16Sr I组中的各植原体同源率均达到99 %以上，其中与16Sr I中的白蜡树丛枝（Ash witches'-broom，AWB，AY566302），柳叶菜变叶（Epilobium phyllody，EP，AY101386）和苦楝丛枝江西株系（Chinaberry witches'-broom，CWB-JX，AY859543）的同源率最高，达到100 %，而与其它组的植原体16S rDNA序列的同源率均低于97 %。

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中，Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带，即Wx-A1α基因出现；Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带，在缺失类型中扩增出一条574bp的特异带。

And it was divided into four groups at randomly (n=6),The vertical compression,bending and torsion intensity in without and with reduction of cortical fragment of plate fixated contra side, without and with reduction of cortical fragment of plate fixated sidewere measured.A11 results were analyzed with statistic method.2、animal experiment :The 12 adult goats were divided into two group at randomly,wedged cortical defect with 1 cm of long and conjugate diameter was created in the middle-part of the medial femur and plate was fixed in the defect side and as the experimental side,the other side was control.after operation 4、8、12 weeks,investigation of the fracture healing by means of gross observation,X-ray examination and histology in both sides.

测试各组的垂直压缩、三点弯曲及抗扭转的刚度。2、动物实验：12只健康成年山羊，雌雄不限，体重在20～25kg之间，在同一只羊双侧股骨中段内侧制造楔形骨皮质缺损，分实验组和对照组两组进行钢板内固定并植骨，于术后4、8、12周对实验组及对照组行X线正位片检查，观察骨折愈合过程中不同时间点的影像学特点。于4、8、12周各组处死4只动物行大体标本观察，并取缺损区骨组织行组织学检查。X线检查及组织学检查结果根据LaneX线评分标准及组织学评分标准进行评分。最后采用SPSS13.0进行统计学分析。

For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。