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We obtained a 1.5Kb DNA fragment of the succinate dehydrogenase flavoprotein subunit by PCR amplification and carried out the homolog analysis with those of other organisms.The result indicated a high homologh in this fragment among the five organisms.

通过PCR方法得到了琥珀酸脱氢酶黄素蛋白基因的一个1.5KB大小的DNA片段，并与其它生物的同种酶的基因序列进行了同源性分析，发现在所比较的五种不同生物之间这个区段具有很高的同源性。

These rocks are lack of foliation and resulted from the brittle fragment ation of mineral grains with rotation of grain fragment s accompanied by fractional grain boundary sliding and dilatancy.

这类岩石缺乏面理构造，是由于矿物颗粒的脆性碎裂，旋转以及颗粒边界的磨擦滑动和扩展而造成的。

The results indicate that the cone half angle increases with liquid pressure. The stripped half wavelength liquid fragment seems to break into a series of drops immediately and no obvious contraction from liquid fragment to ligament is seen.

研究结果表明，液膜半锥角随着液压的增加而增加，半波长的液膜段剥落后几乎立即破碎成液滴，看不到明显的液膜段收缩成液丝的过程。

We cloned a gene fragment which contain a disintegrin domain from Agkistrodon halys by RT-PCR methods, use the distinguish sequence of this fragment as primer we clone the full length gene.

我们利用RT-PCR方法从江浙蝮蛇中克隆出一个含去整合蛋白结构域的基因片段，从测定的片段序列中取出特异的序列作为引物获得它的全长基因。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

After sequenced the band, we found that no fragment is isogeny with it in NCBI date base. It shows that the DNA fragment was a new cucumber DNA sequence.

将p63-m51-384特异条带进行测序，采用NCBI的Blast软件对GeneBank中的非冗余数据库(GeneBank+EMBL+DDBJ)进行查询，没有发现与该标记片段同源性高于30%的片段，说明该片段为新发现的黄瓜序列。

The fragment m/z 132 is 19 amu above the standard fragment mass for leucine or isoleucine, respectively; therefore, one of these amino acids has to be the C-terminal part of the dipeptide.

破片 m/z 132 分别比亮氨酸或亮氨酸残留物的标准破片质量多19个原子质量单位。因此，这些氨基酸中的一个个只能是二肽中的羧基末端。

The AC2 gene fragment of cotton leaf curl virus was obtained from total DNA of CLCuV infected tobacco leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into vector.

以棉花曲叶病毒侵染的烟草叶片组织总DNA为模板，通过聚合酶链反应扩增CLCuV的AC2基因片段并插入克隆载体。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants.

我们将RRD3片段插入到植物启动子捕获载体pCAMBIA1391Z中，检测RRD3片段是否在植物中也有启动子活性，转基因烟草再生植株和水稻愈伤组织均显示了gusA基因的表达，表明其在单子叶和双子叶植物中均可行使启动子功能。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。