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As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

By analyzing the tissue-specific expression in 35-day-old plants, AtMAPR2 was expressed higher in flower, rosette leaf, and silique; while AtMAPR3 was expressed higher in flowers, roots, rosette leaf; AtMAPR4 and AtMAPR5 were expressed higher in flowers.

而三十五天的器官特异性分析，可观察到AtMAPR2对花、丛生叶与长角果；AtMAPR3对花、根、丛生叶；AtMAPR4、AtMAPR5对花具有器官特异性。

AR expressed stronger in spermatocytes and spermatogenic cells, ER in germ cells and sperm expressed strong in the second spermatocyte, PR in Leydig cells also expressed stronger.

AR在精母细胞和精细胞中表达较强，ER在精细胞和精子中表达较强，精母细胞中次之，PR在睾丸间质细胞中表达较强。

We detected that EGF mRNA was expressed sflungly lii the oocyte, and is also found hi gmnulosa cells, the cell fium smaller foflicular expressed stronger than fium bigger one. In the corpus hemonbaglcwn corpus luteurn, lean type and pseudocorpus-luteum, EGF rnRNA was detected,, no distinct difference can be seen in them. The EGF mRNA expressed strongly in fimbria end, ampulla and isthmus of oviduct, in the big follicular stage, ovulation stage, pregnancy stage and spurius pregnancy stage, we can not see any distinct change in them, but hi the medium follicuar stage,it is weaker.

结果发现：猪卵母细胞中EGF的mRNA强烈表达，且小卵泡卵母细胞→中卵泡卵母细胞→大卵泡卵母细胞中，EGF的mRNA表达量有逐渐减少的趋势；猪卵泡的颗粒细胞中有EGF的mRNA表达，小卵泡颗粒细胞→中卵泡颗粒细胞→大卵泡颗粒细胞中，EGF的mRNA表达也有逐渐减少的趋势；猪卵巢中的红体、黄体、白体和假黄体中都有EGF的mRNA表达，看不出几部分的表达量有明显的强弱变化；猪输卵管伞部、壶腹部和峡部，都有EGF的mRNA表达，在大卵泡期，排卵期，孕期和假孕期都强烈表达，各期间看不出明显的强弱变化，中卵泡期表达较弱；猪子宫中EGF的mRNA在大卵泡期，排卵期，孕期和假孕期都强烈表达，看不出表达量的明显变化，而小卵泡期表达量明显减弱。

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明：经重组质粒的构建获得特异表达于人胎盘的p100－GAP的cDNA探针后，从蛋白和分子水平证实p100－GAP大量表达于分化程度高的合体滋养层细胞，少量表达于体外培养的中间型滋养层细胞，而非分化的细胞滋养层细胞NPC则不表达，这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致；p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

T was later transiently expressed in pharyngeal arches, and in pectoral fin buds. IGFBP-3 mRNA is predominantly and persistently expressed in the developing otic vesicle. IGFBP-3 is also expressed in optic stalk, retina, pineal gland, tegmentum in midbrain, anterior pituitary and midline floor of hypothalamus.

GFBP-3 mRNA 持续而强烈地表达在发育中的内耳，它也表达在眼睛的视杆和部分视网膜细胞，松果腺以及脑垂体前部、中脑脑盖和下丘脑的基底中央等脑组织。

The number of differentially expressed genes was 71 at 24h, and 62 at 72h. The metabolisic gene class was the majority among all the differentially expressed genes, and the next was transportational、differentiation and adhesional gene class by turns.1.2. RT-PCR verification of the known HIF-1 targeted genes and proliferation related genesIn the 131 differentially expressed genes, we found 7 known HIF-1 targed genes (ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3) and 5 genes (dnaja2、ccnb1、tgfβ2、ccng1、rgs7) which were related to proliferation and cell cycle.

对差异表达的基因进行基因功能分类发现：代谢类基因最多，其次是运输类基因、细胞分化及黏附类基因。1.2 RT-PCR验证HIF-1靶基因和增殖相关的基因由于我们的前期实验证明了低氧促NSC增殖是有HIF-1参与的，我们对131个差异表达基因中，7个已知的HIF-1靶基因(ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3)和5个与细胞周期和增殖相关的基因(dnaja2、ccnb1、tgfβ2、ccng1、rgs7)做RT-PCR验证。

Among the 3 novel sequences, C24 sequence and C57 sequence were expressed both in cataractous group and in normal group; C24 sequence was expressed higher in cataractous group than that in the normal group, but C57 sequence had no significant difference in normal and cataractous group; C40 sequence was expressed in the cataractous lens epithelium but the expression in normal lens epithelium was not seen.

三个新序列中，C24号和C57号序列在年龄相关性白内障和正常晶状体上皮细胞中均有表达，C24号序列的表达增高；C57号序列在正常组和白内障组中的表达无差异。C40号序列在年龄相关性白内障晶状体上皮细胞中有表达，但其在正常晶状体上皮细胞中未见表达。

The results showed that 17 gene fragments were expressed exclusively,26 gene fragments expressed increasingly,and 20 gene fragments expressed decreasingly in incompatible combination compared with either in compatible combination or in control.

结果表明，非亲和性互作中特有的表达片段7条，较亲和性互作及对照表达明显增强的片段21条，表达明显减弱的18条。

There was no expression of CTGF protein in the pulmonary arteriole of the normal group, whereas, it was highly expressed in the tunica adventitia, slightly expressed in the endangium and rarely expressed in the tunica media of pulmonary arteriole in the hypoxemic group.

正常对照组大鼠肺小动脉壁CTGF蛋白未表达；低氧组大鼠肺小动脉壁外膜CTGF明显表达，内膜略有表达，中膜几乎无表达。

I smile at your play with that little bit of a broken twig.

我微笑着看你玩那一段小小的树枝。

They may be produced for almost any shaft angle.

他们可能在制作中几乎任何一个轴角。