查询词典 expressed

Methods HIV-1 env gp41 gene of BH10 strain (nt6 977-7 497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

表达产物通过15%SDS-聚丙烯酰胺凝胶电泳初步分离纯化，根据RF值，切下含特异蛋白的胶带，以Western blot法转移在硝酸纤维素膜上；免疫血清法检测合格者制备的抗原检测条带，经国家标准参比血清检测。

A prokaryotic expression vector pET28a-exchIL-2Rγ was constructed successfully and expressed, and the expressed protein was identified.

构建了鸡IL-2Rγ胞外区的原核重组表达载体，进行了表达和鉴定。

Fifty-eight different proteins were observed between the normal group and Pulsatilla chinensis group with qualitative analysis, of which 45 proteins were expressed only in the normal group, 13 protein was expressed only in the Pulsatilla chinensis group; 43 spots were found increased that showed a two fold increase and 50 spots were found decreased that showed 50% decrease at least as comparing to normal group with quantitative analysis.

定性分析，正常组和白头翁组共有58个表达差异点，其中只在正常组中表达的点有45个，只在白头翁组表达的点有13个；定量分析，白头翁组和正常组比较，表达增加大于2倍的点有43个，降低50%以上的点有50个。

The subjective preference information is expressed by a fuzzy preference complementary matrix of the decision alternatives and a pairwise comparison reciprocal matrix of the weights of attributes, and the objective information is expressed by a objective decision matrix.

主观偏好信息由决策方案的模糊偏好互补矩阵和属性权重的两两比较互反矩阵组成，客观信息由客观决策矩阵组成。

Northern blot revealed that DAT is expressed weaklt but specificlly in the brain tissue, and in situ hybridization DAT is restrictedly expressed in the adult brain tissues including substantia nigra and striatum, suggesting an important function in the dopaminergic system.

利用cRNA探针的原位杂交组织化学研究，发现DRP在成年大鼠脑组织神经元和胶质细胞均有表达，以胶质细胞广泛表达是其重要特征；而DAT在成年脑组织仅限于少部分核团的有限表达，包括黑质和纹状体。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

In his literary and dramatic works, Lessing expressed the thoughts to Enlighten and foster the national sprit and even to set up an integral national civilization; what"s more, he opposed the feudal separationist rule with his ideal of Kosmopolitismus. At that time, after succeeding and developing European humanist tradition, Lessing formed his religious philosophy and religious tolerance, with his rational opinion, he deepened the profundity of German Enlightenment. Furthermore, he created townspeople"s drama and advocated the realistic aesthetics. On the one hand, he praised their disposition and expressed their political desire and class interest, which made the townspeople as a new motive force to the German Enlightenment. In addition, Lessing"s Enlighten. thoughts greatly influenced the following movement—Sturm Und Drang. The essence of Lessing"s thoughts is our common valuable prosperity.In a word. Germany then greatly needed a person like Lessing, and Lessing was the child of German Enlightenment.

面对法国文化长期统治德国文化界的现状，莱辛勇敢地站出来反对"法国化"倾向，对德意志全社会进行民族启蒙，通过戏剧理论及作品，表达了建立统一的民族文化、培植德意志民族精神的思想，同时他提倡世界主义，并用它来为民族主义服务，以反对当时德国的分裂割据；他的宗教哲学思想及宗教宽容精神继承和发扬了西欧的人文主义传统，反映了莱辛的理性观，也针砭了当时德国的时弊，是他对德国启蒙运动进行的有益的探索，拓宽了德国启蒙运动的深度；莱辛在实践中创作了市民剧，他的市民主义和现实主义美学思想弘扬了市民阶级的性格，表达了他们的政治愿望和阶级利益，为德国启蒙运动增加了新的动力，是莱辛所作的杰出贡献之一；莱辛的启蒙思想对后世特别是狂飙突进运动有着重大的影响，他的思想中的精华部分是人类的共同财富。

It is shown from the detection results that VAMP-2 is expressed in intestine, liver, kidney, skin, spleen and sex gland as well as brain of Japanese sea perch at different levels, but no VAMP-2 is expressed in...

结果表明，除脑组织外，在肠、肝脏、肾脏、皮肤、脾脏、性腺等组织中也检测到 VAMP-2的表达，各组织间的表达水平亦存在差异，肌内组织未检测到表达。

With these words, the Shulamite not only expressed her undying love for her husband, but she also expressed her faithfulness and commitment to him.

由这些话，书拉密女不只说出她对丈夫不会改变的爱，也说出她的忠心和承诺。

FOXC2 protein and mRNA were extensively expressed in pregnant 40 day embryonic somite and neural tube mesoderm which would form cardiovascular and axial skeleton primordia, especially strong expressed in the cells of mesenchyme surrounding notochord and dorsal aorta approaching the head.

这个基因广泛表达在孕40天胚胎体节和神经管周围未来分化为心血管和中轴骨骼始基的中胚层，靠近头部围绕脊索和背主动脉的间充质细胞核中表达强烈。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。