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Catenin was detected in suprabasal, epidermal basal layer, placode, hair bud in early phase(E68～93),and expressed strongly in epidermal basal layer, placode, and hair bud, in suprabasal expressed less strongly; in metaphase(E94～184),β-catenin was detected in epidermis, hair peg, and in suprabasal, epidermal basal layer, hair follicle bulge, inner root sheath, outer root sheath, follicular infundibulum expressed less strongly; in late phase(E184～225),β-catenin expressed weakly in epidermal basal layers, while expressed strongly in epidermal keratinocytes.

结果显示：β-catenin在胎牛蹄边缘毛囊形态发生早期(68～93天)表达于表皮、基底层、基板和毛芽中，其中基底层、基板、毛芽中呈强阳性，表皮中呈中阳性；在毛囊形态发生中期(94～184天)表达于基底层、表皮和毛钉中，其中基底层、表皮、隆突区、内外根鞘和漏斗部呈中阳性；在毛囊形态发生晚期(184～225天)只在表皮及基底层有表达，其中基底层呈弱阳性，表皮呈强阳性。

Those DNA bands expressed in female parent but not in male parent also expressed in gynogenesis filial; DNA bands expressed in male parent but not in female parent didn抰 express in gynonensis, while normally expressed in dcploid control fish.

证明牙鲫雌核发育子代的遗传物质来自母本，雌核发育诱导使用的精子经过紫外线灭活后，遗传物质己被破坏，其携带的遗传信息没有传给子代，在DNA水平证明了雌核发育诱导方法和结果的可靠性。

Five floral organ identity genes of Mussaenda pubescens were cloned and their expression patterns among different organs were detected by RT-PCR. The AP1 homologue, MupAP1, is expressed in enlarged and small sepals, petals and stamens, carpels and fruits; the SEP3 homologue, MupSEP is strongly expressed in all floral organs and weak in leaves as well. Two of the B class gene, AP3 homologue MupDEF and TM6 homologue MupTM6, were detected expressed in all floral organs, however, the other B class gene, MupGLO, homologous to PI, were detected expressed in small sepals, petals and stamens, carpels and fruits, but not in enlarged sepals.

本论文选殖出五个毛玉叶金花花器同源基因，其中A群基因AP1的同源基因MupAP1在两型花萼、花瓣及雄蕊、心皮、果实中有表现，E群基因SEP3的同源基因MupSEP则在所测部位均有表现，B群基因中，AP3的同源基因MupDEF及TM6的同源基因MupTM6在两型花萼、花瓣及雄蕊、心皮及果实中均有表现，另一个B群基因，PI的同源基因MupGLO则在花瓣及雄蕊、心皮、果实及小花萼中有表现，但在膨大花萼中并没有侦测到表现。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

With the development of embryo, Myf5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites; After 30 somites formed, MyoD expression decreased in the somites except the caudal somites. At the hatching stage, MyoD and Myf5 were expressed in head muscle cells and fin muscle cells. In the growing fish, Myf5 was expressed in the skeletal muscle and intestine, and in adult flounder, Myf5 was only expressed in muscle. In the growing fish and adult fish, MyoD was only expressed in muscle.

在胚胎发育早期，Myf5在近轴中胚层中表达，体节发生过程中，Myf5在体节中表达，MyoD基因最早在分节板的体节前细胞中表达，随后在近轴细胞、体节中表达；随着胚胎的发育，Myf5在成熟体节中表达量降低，在新生体节中表达较强；MyoD自30个体节时期后只在新生的尾部体节中表达，在成熟的体节中表达量降低；在孵化期，MyoD和Myf5在头部及鳍的肌肉、尾部的体节中表达；生长期的牙鲆中，Myf5在骨骼肌和肠中表达，成体牙鲆中，Myf5只在肌肉中表达；生长期的牙鲆及成体牙鲆中，MyoD只在肌肉组织中表达。

At 24 h, Ang1 was expressed mainly in the lens, optic cup, mesencephalon, ventral part of hindbrain, and myotomes in the somites, while zfTie2 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk. VEGF was expressed mainly in the lens, optic cup, mesencephalon and myotomes in the somites, while zfFlk1 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk.

结果从受精卵胚胎发育第12小时可见血管内皮细胞生长因子和血管发生素1及其相应受体flk1和Tie2的表达；在受精卵胚胎发育的第16小时，血管内皮细胞生长因子和血管发生素1主要在头部和尾部胚芽表达；第24小时在眼球、视窝、中脑、后脑的腹前侧和肌节中表达，Tie2和Flk1主要在肌间背部动脉血管、躯干新生血管芽和轴向静脉中表达。

Longan1 expressed from the embryogenic callus stage to the globular embryoid stages. Longan2 expressed in the whole process of longan somatic embryogenesis, which was probably a housekeeping gene. Longan3 expressed from the globular embryoid stage to the torpedo-shaped embryoid stage. Longan4、longan4'expressed only at the cotyledonary embryoid stage.

碱基序列分析表明，longan1在3'端存在一个开放阅读框，longan2、longan3分别在5'端存在一个开放阅读框，longan1在3'方向，longan2、longan3在5'方向均没有终止密码子，所以可能往相应的方向延伸仍为各自的ORF，因此可以通过RACE或者筛选cDNA文库的办法来获得它们的全长cDNA。

In immunohistochemistry slices of skeletal muscle injury induced by contusion, the expression conditions of MyoD protein (one molecule symbol during the differentiation of sarcoblast) in satellite cells are followings: it is expressed continuously in gastrocnemius of treated group at 24 hours、2 days and 4 days after injury positive it is expressed at 4th day in fast muscle of untreated group; it is expressed at 2th day after injury in soleus (slow muscle of treated group; it is negative in slow muscle of untreated group.

由此可见，云南白药在骨骼肌损伤后的修复过程中的作用是通过抑制炎性细胞对伤后肌肉组织的破坏作用，缩短炎症反应进程，对骨骼肌卫星细胞的增殖分化有一定的促进作用，并且该作用在快肌纤维上表现较为显著，从而有利于损伤肌组织的修复。

According to the studied results at present, the studied objects are mainly thin plate and beam about sound radiation mode, a method computed sound radiation mode of complex structure is presented in this dissertation, according to the results of the previous two chapter, a theoretical method to solve acoustical radiation mode and acoustical radiation efficiency of complex structure by boundary element method and acoustical radiation theory is posed, the acoustical radiation power is expressed as a Hermitian quadratic form, while the radiation modes is determined by general eigenvalue decomposing, and then the radiation efficiency is computed via the orthodoxy of radiation modes with regard to impedance matrix and average velocity matrix; lastly, the validity of the method is proved by pulsating sphere and radiating cube with analytical results. According to results of the form three chapter, the structure sound radiation sensitivity is studied in detail, the sound radiation power of structure can be expressed as positive Hermitian quadratic form, the sound radiation sensitivity can be expressed as two parts by partial differential with respect to design variable, which are sensitivity of boundary velocity and impedance matrix, combined with the theory of FEM and BEM, the structure sound radiation can be translated to the analysis of structure dynamic sensitivity and impedance matrix sensitivity. The theory posted in this dissertation is tested by the FEM software ANSYS and the software AAS programmed by author.

在前几章的基础上，通过结构声辐射的模态理论对结构的声辐射的机理进行了深入地探讨，针对目前声辐射模态的研究对象主要是简单的板和梁类结构，提出了一种计算复杂结构声辐射模态的方法，利用前两章研究所得的结论，将边界元方法与广义特征值的理论结合起来研究了复杂结构的声辐射模态与声辐射效率，先将结构的声辐射功率表示为一个正定的厄米特二(来源：ABb7C论文0909网www.abclunwen.com)次型，运用广义特征值分解求解了复杂结构的声辐射模态，然后利用声辐射模态关于阻抗矩阵与均方速度耦合矩阵的正交性，求解了复杂结构的声辐射效率，最后用具有解析解的脉动球与辐射立方体验证了该方法的有效性。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。