查询词典 expressed

Paternally expressed gene 10 (PEG10) is expressed in HCC cell lines at substantial high level.

肝细胞癌细胞株管家基因10（PEG10）表达水平较高。

Paternally derived FAC1 gene is expressed at the zygote stage, which counts as the earliest paternally expressed gene known in plants.

父源的FACl基因的表达在合子中即可被激活，这是已知的最早表达的父源基因。

IGF2, which encodes a protein functioning as a fetal growth factor and a cell mitogen, is expressed only from paternally allele in most tissues but from both alleles in adult liver. H1 9, which acts as an RNA, is a gene of unknown function and expressed only from maternally allele.

IGF2编码的胰岛素样生长因子-2是一种胚胎生长因子及细胞有丝分裂原，在人体内大多数组织中特异地表达父系来源的等位基因，而母源等位基因处于印记状态。H19表达一种功能尚不清楚的RNA，其印记状态与IGF2相反，目前发现H19在正常组织内均为母源等位基因表达，父源等位基因印记。

Here we report a total of 39.9 Mb of expressed sequence tags from 29 animals belonging to 21 phyla, including 11 phyla previously lacking genomic or expressed-sequence-tag data.

在这里，我们报告从29属21门动物，其中包括11门动物以前所缺乏的基因或表达序列标记的数据动物的39.9表达序列标签MB的总数。

Methods HIV-1 env gp41 gene of BH10 strain (nt6 977-7 497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

表达产物通过15%SDS-聚丙烯酰胺凝胶电泳初步分离纯化，根据RF值，切下含特异蛋白的胶带，以Western blot法转移在硝酸纤维素膜上；免疫血清法检测合格者制备的抗原检测条带，经国家标准参比血清检测。

A prokaryotic expression vector pET28a-exchIL-2Rγ was constructed successfully and expressed, and the expressed protein was identified.

构建了鸡IL-2Rγ胞外区的原核重组表达载体，进行了表达和鉴定。

Fifty-eight different proteins were observed between the normal group and Pulsatilla chinensis group with qualitative analysis, of which 45 proteins were expressed only in the normal group, 13 protein was expressed only in the Pulsatilla chinensis group; 43 spots were found increased that showed a two fold increase and 50 spots were found decreased that showed 50% decrease at least as comparing to normal group with quantitative analysis.

定性分析，正常组和白头翁组共有58个表达差异点，其中只在正常组中表达的点有45个，只在白头翁组表达的点有13个；定量分析，白头翁组和正常组比较，表达增加大于2倍的点有43个，降低50%以上的点有50个。

The subjective preference information is expressed by a fuzzy preference complementary matrix of the decision alternatives and a pairwise comparison reciprocal matrix of the weights of attributes, and the objective information is expressed by a objective decision matrix.

主观偏好信息由决策方案的模糊偏好互补矩阵和属性权重的两两比较互反矩阵组成，客观信息由客观决策矩阵组成。

Northern blot revealed that DAT is expressed weaklt but specificlly in the brain tissue, and in situ hybridization DAT is restrictedly expressed in the adult brain tissues including substantia nigra and striatum, suggesting an important function in the dopaminergic system.

利用cRNA探针的原位杂交组织化学研究，发现DRP在成年大鼠脑组织神经元和胶质细胞均有表达，以胶质细胞广泛表达是其重要特征；而DAT在成年脑组织仅限于少部分核团的有限表达，包括黑质和纹状体。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。