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METHODS: BMSCs, CCs, and ADAS were primary-cultured from goat bone marrow following trypsin digestion and amplified in monolayer.

抽取羊骨髓,胰酶消化后获得原代骨髓间充质干细胞细胞、软骨细胞和脂肪源性成熟基质细胞,单层培养扩增。

To export whether the ovariectomy induced increase in bone resorbing activity present in supernatants fraction of bone marrow and the effects of endogeneous IL-6 on bone resorption, the bone resorbing activity present in marrow supernatants and the neutralizing effects of antibody against IL-6 were examined by the release of 〓Ca from perlabeled fetal rats parietal bone, the concentration of IL-6 were measured by Enzyme-Linked Immunosorbent Assay .

为了探讨卵巢切除是否导致骨髓上清骨吸收活性增加,以及白细胞介素-6在骨吸收中的作用,通过胎鼠颅骨〓释放实验测试骨髓上清的骨吸收活性及IL-6抗体的中和作用,通过ELISA测骨髓上清IL-6浓度。

Biochemical indicator such as TG and SF were unregulated while 1 system or 2 system reduced in peripheral blood; Bone marrow hyperplasia showed active or active obviously. Complete morphological nucleated cells mature erythrocytes or platelet hemophagocytic cells were found in 100% marrow slides. Marrow hemophagocytic cells were quantified as 0.5 to 11 per micro liter Median 4.2 per micro liter. Phagocytes showed smaller size single nucleus on one side. Submitting as rings cell plasm showed gray or salmon pink and cell showed disorder fringes.

结果: 符合HPS者30例诊断为HPS早期21例;HPS早期有持续高热峰值≥38.5 ℃、发热期≥4天发病与感染相关;以病毒感染最多见,生化指标TG增高、SF增高;外周血细胞一系或二系减低;骨髓增生活跃或明显活跃,骨髓片(100%)找到吞噬了完整形态的有核细胞、成熟红细胞或血小板的噬血细胞骨髓噬血细胞0.5-11个/ul中位数4.2个/ul吞噬细胞体积偏小多为单个核核偏一侧,可呈印戒样,胞浆灰兰或淡红色,细胞边缘不整齐。

Biochemical indicator such as TG and SF were unregulated while 1 system or 2 system reduced in peripheral blood; Bone marrow hyperplasia showed active or active obviously. Complete morphological nucleated cells mature erythrocytes or platelet hemophagocytic cells were found in 100% marrow slides. Marrow hemophagocytic cells were quantified as 0.5 to 11 per micro liter Median 4.2 per micro liter. Phagocytes showed smaller size single nucleus on one side. Submitting as rings cell plasm showed gray or salmon pink and cell showed disorder fringes.

结果: 符合HPS者30例诊断为HPS早期21例;HPS早期有持续高热峰值≥38.5 ℃、发热期≥4天发病与感染相关;以病毒感染最多见,生化指标TG增高、SF增高;外周血细胞一系或二系减低;骨髓增生活跃或明显活跃,骨髓片(100%)找到吞噬了完整形态的有顾细胞、成熟红细胞或血小板的噬血细胞骨髓噬血细胞0.5-11个/ul中位数4.2个/ul吞噬细胞体积偏小多为单个核核偏一侧,可呈印戒样,胞浆灰兰或淡红色,细胞边缘不整齐。

In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7%of endogenous murine α-globin gene.

5%。在2只长期造血重建小鼠的骨髓、脾、胸腺及另一只小鼠的骨髓及脾中整合有人β-珠蛋白基因,在一只重建小鼠的骨髓和脾中有人β-珠蛋白基因表达,约为鼠α-珠蛋白基因的7 %。

Results The tail formation rate of model group was significantly higher than the rate of control group (P<0.05) with a large number of Ⅲ and Ⅳ DNA damage cells. The tail formation rate of dichloromethane extraction groups were both significantly lower than the rate of model group (P<0.05). p53 mRNA expression of model group was higher than the rest except the crude Cornus Officinalis group.

结果 D-半乳糖致衰模型组小鼠骨髓细胞DNA拖尾率高于正常对照组(P<0.05),且Ⅲ、Ⅳ级损伤细胞数较多;山茱萸生品及制品二氯甲烷萃取部位组小鼠骨髓细胞DNA的拖尾率均低于D-半乳糖致衰模型组(P<0.05)。D-半乳糖致衰模型组小鼠骨髓细胞p53基因的表达高于正常对照组、阳性药组及制品组。

Method]Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法]rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养,应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子,并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物,直接种植到兔膝关节实验性关节软骨缺损处,并与对照组相比较,观察软骨修复效果。

It is concluded that the expression of hERMAP in fetal liver approximately consistent with haematopoiesis of fetal liver, and the expression of this gene in bone marrow aged 15-32th fetal weeks coincides with haematopoiesis of fetal bone marrow.

人类ERMAP基因在胎儿肝脏中的表达规律与胎儿肝脏造血过程基本一致,在15-32周胎儿骨髓中的表达规律与胎儿期骨髓造血活动基本吻合,推测该基因的功能可能与胎儿发育过程中红系细胞向肝脏和骨髓的迁移活动有关。

Blood production - The marrow, located within the medullary cavity of long bones and the interstices of cancellous bone, produces blood cells in a process called haematopoiesis.

造血-骨髓位于长骨髓腔和松质骨间隙内,骨髓通过造血作用产生血细胞。

L Blood production — The marrow, located within the medullary cavity of long bones and the interstices of cancellous bone, produces blood cells in a process called haematopoiesis.

l 造血-骨髓位于长骨髓腔和松质骨间隙内,骨髓通过造血作用产生血细胞。

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