骨髓

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

It also is related to bone marrow structural damage resulting from tumor invasion,large proliferation of granulation or fibrous tissue,which often affect hemopoiesis.Leukemoid reaction occurred in the majority of malignancies is myeloid leukemia response,among which some are.neutropenia,and even agranulocytosis and increasion or decreasion of platelets.These are all have close relationship with marrow invasion of malignant tumor,immune reaction of the body and chemotherapy as well as radiotherapy.

亦与骨髓被恶性肿瘤细胞浸润、肉芽和纤维组织大量增生，使骨髓结构破坏，影响红系造血有关；绝大多数恶性肿瘤并发的类白血病反应是粒细胞性的，部分患者白细胞减少，甚至粒细胞缺乏，以及血小板增高或减少，均与恶性肿瘤细胞骨髓浸润、机体的免疫反应、放化疗有关。

The hypocellularity in bone marrow smear is not caused totally by hypoplasia. Combining the two methods, bone marrow aspirate and trephine biopsy can increase the accuracy diagnosing the cellularity in BM and hematological diseases.

骨穿干抽或骨髓涂片示细胞增生低下并非完全是骨髓细胞增生不良所致，骨髓涂片与活检相结合可提高对骨髓细胞增生程度的判断及血液病诊断的正确性。

Full-thickness articular cartilage defects (diameter: 3mm, depth: 3mm) were made to bilateral condylus medialis femoris of all rabbits. In periosteum+BMSCs group and periosteum group, defects were covered by homolateral autogenous proximal tibia periosteums, with germinal layer facing to cavitas medullaris. After that, the periosteum+BMSCs group received 3 sutures, followed by injection of 20 μL BMSCs suspension (1×109/L) into the defects, after which the last suture was taken.

在全部兔双侧股骨内侧髁造成直径3 mm，深度3 mm的全层软骨缺损，骨膜复合骨髓间充质干细胞组、单纯骨膜组取同侧胫骨上段内侧骨膜，骨膜生发层朝向骨髓腔覆盖软骨缺损，骨膜复合骨髓间充质干细胞组先期缝合3针，向缺损区注入1×109 L-1骨髓间充质干细胞细胞悬液20 μL，植入细胞后缝合最后1针。

BMSCs and recombinant human GSF (50 μg/kg) were injected into models by tail vein and hypodermoclysis infusion respectively in the combination treatment group. Recombinant human GSF (50 μg/kg) was subcutaneously injected into models in the bone marrow stem cell autologous mobilization group. BMSCs were injected into rat models by tail vein in the BMSC group. Rat models in the heart failure group were intact.

造模后随机分成4组：联合治疗组尾静脉注射骨髓间充质细胞和皮下注入重组人粒细胞刺激因子50 μg/kg，自体骨髓干细胞动员组皮下注入重组人粒细胞刺激因子50 μg/kg，骨髓间充质干细胞组为尾静脉注射骨髓间充质细胞，心力衰竭组不给予任何干预。

To investigate the morphological changes of megakaryocytes with nuclear extrusion and nucleocytoplasmic separation, the morphological characteristics of megakaryocytes in peripheral blood films, bone marrow smears, and bone marrow biopsies from 4 newly diagnosed patients with primary myelofibrosis, myelodysplastic syndrome, myeloblastic leukemia with maturation (M2) and erythroleukemia (M6) were studied by using light microscope.

为了观察巨核细胞逸核和胞核胞质连体分离的形态学变化，用光镜观察4例初诊血液病患者的血片、骨髓涂片和骨髓切片中巨核细胞形态特征。4例初诊血液病患者为1例原发性骨髓纤维化，1例骨髓增生异常综合征，1例急性粒细胞白血病部分成熟型(M2)和1例红白血病(M6)。

Methods Six weeks old male Fischer 344 rats (F344) were used as donors, and six weeks old male F344alb as recipients. All F344alb were divided into 4 groups: group Ⅰ(n=10) was untreated; group Ⅱ(n=10) was treated with CCl4 but no γ-irradiation and transplantation; group Ⅲ(n=10) was transplanted with 1×10^7 BMCs after γ-irradiation, but without CCl4 treatment; group Ⅳ(n=10) was transplanted with 1×10^7 BMCs after γ-irradiation, with CCl4 treatment. The BMCs prepared from the donors were infused via the penial veins of recipients immediately after whole body γ-irradiation (7.5Gy) and CCl4 treatment: 4 weeks after BMCs transplantation, CCl4 were injected into peritoneal cavity twice a week for 4 weeks.

雄性F344大鼠为供体，而雄性F344alb为受体，分为4组：组Ⅰ(n=10)为正常对照组，不接受肝损处理，无全身射线照射和阴茎静脉骨髓细胞移植；组Ⅱ(n=10)为肝损无移植组，只接受肝损处理，无全身射线照射和阴茎静脉骨髓细胞移植；组Ⅲ(n=10)为正常移植组，全身射线照射后阴茎静脉移植骨髓细胞1×10^7；组Ⅳ(n=10)为肝损移植组，接受CCl4注射肝损，全身射线照射后阴茎静脉骨髓细胞移植1×10^7BMCs，其中，γ-照射剂量为7.5Gy，F344大鼠的BMCs经受体的阴茎静脉注入。

Pathological observations showed that the bone marrow and spleen of non-treated IMAA mice demonstrated typical aplastic anemia pattern, including bone marrow failure, marked splenatrophy, but the bone marrow and lymphoid tissues in the IMAA mice of irradiation-treated group were recovered to normal at day 28 after treatment.

结果表明：①IMAA组小鼠活存率为12.5%，平均存活时间为27.4 13.4天，照射治疗组活存率为100%，平均存活时间60天以上，单纯照射组无死亡；②外周血白细胞数：IMAA组呈进行性下降，直至死亡，照射治疗组第10天与免疫再障组相似，以后开始缓慢回升，基本上达到治疗前水平；③红细胞比容：未治疗IMAA组于14天后呈进行性降低，至第35天比实验前减低2/3，照射治疗组与单纯照射组一样，第14天有轻度减低随后升高，至35天接近正常；④骨髓有核细胞数：IMAA组呈进行性减低，无恢复趋势，照射治疗组于一过性减低后迅速增加，第28天达到正常水平；⑤骨髓和淋巴组织病理形态观察：IMAA组小鼠呈典型再生障碍性贫血病理改变，骨髓造血衰竭，脾脏明显萎缩，而照射治疗组于第28天骨髓和脾淋巴脏组织基本上恢复正常。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中，与血清浓度为0的浓度组相比，与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少，且随血清浓度的增加，小鼠骨髓cfu-e和bfu-e的集落数逐渐减少，各浓度组间差异有显著性（p＜0.05）。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成，随患者血清浓度的增加，抑制作用增强，具有剂量依赖性。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看，因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。