酵母

Yeast also is an important research material and provides a detectable experimental system in genetics and molecular biology. The functional complementation assay of homeotic gene re-expression in yeast mutant has become a screen tool in mechanism research of drug.

第三章spm1、pmp1和mid2基因在相应粟酒裂殖酵母突变体中的再表达利用酵母突变体及其同源基因再表达的功能互补实验，是目前遗传学和分子生物学研究的一种重要实验方法，并使酵母成为筛查药物作用机制的工具。

Unsurprisely,These three microorganisms decreased as the storage time increases.84 strains lactobacillus were isolated during the detection period in the pickled gingers stored in low temperature.By physiology and biochemistry methods,32 strains L.plantarums,6 strains L.brevis,2 strains Lacto...

低温保存泡姜在整个检测期内，共分离到84株典型乳酸菌，经生理生化实验鉴定出植物乳杆菌32株、弯曲乳杆菌7株、短乳杆菌6株、肠膜明串珠菌属5株、乳球菌属2株、乳杆菌属32株；酵母菌分离鉴定出3个属，主要为酵母属以及少量的假丝酵母属和汉逊氏酵母属；只在保存的前2d检测到个别的青霉属和毛霉属；在整个检测期内，没有分离到大肠杆菌。

The first method adopts different kind microbes to ferment buckwheatextraction. The method can increase DCI and its derivation content in buckwheat seed solution, while itreduces sugar content in extraction. The research adopts Saccharomyces cerevisia, Mucor, Bacterium coli, Torulopsis apicola, Aspergillus oryzae Bacillus subtilis, Candida mycoderma, Black mold that inoculate inbuckwheat extraction. Determine content change of various compotent with tracing.

方法一采用不同种类微生物发酵荞麦提取物，增加荞麦籽粒水溶液中D-手性肌醇及其衍生物的含量，同时减少提取物中总糖含量，本研究采用酿酒酵母、毛霉、大肠杆菌、蜜蜂生球拟酵母、米曲霉、枯草芽孢杆菌、解酯假丝酵母、黑曲霉接种于荞麦提取物中进行培养，并跟踪测定提取物中各种成分的含量变化。

High palmitoleic acid-producing strains were obtained by protoplast fusion between Saccharomyces cerevisiae No.12.926 producing palmitoleic acid and Rhodotorula No.12.908 producing oil and fat.

采用原生质体融合技术进行产棕榈油酸酵母Saccharomy cescerevisiaeNo.12.926和产脂酵母RhodotorulaNo.12.908的融合研究，获得了棕榈油酸高产酵母工程菌株。

The results indicated that whole H-OLE1 gene could complement fad1 mutation in H. polymorpha recipient lacking △9-fatty acid desaturase. However UFA requirement that Saccharomyces cerevisiae ole1 mutant displays was complemented only by ORF of H-OLE1 driven by S.

发现完整的H-OLE1基因可互补缺乏△9-脂肪酸去饱和酶活性的多形汉逊氏酵母营养缺陷型fad1突变体，却不能互补相应的酿酒酵母ole1突变体，而由酿酒酵母GAP表达框架和H-OLE1 ORF组成的嵌合基因可互补上述ole1突变体。

In order to understand the function of this gene within short time, we also constructed yeast (Schizosaccharomyces pombe vector to analyze the gene function, but the result investigated that over expression of this gene could not increase the length of yeast cell. It suggested that expression of the gene is not a direct reason in cell elongation.

为了在短时间内初步研究该基因的功能，构建了酵母表达载体，利用裂殖酵母表达系统对该基因的功能进行活体分析，没有发现该基因对单核的酵母细胞的伸长有明显影响。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

Meanwhile ,those expressing FAE1 genes from Sinapis arvensis, Sinapis alba, and Isatis indigotica produced small quantity of VLCFA which was absent in control yeast cells. The experiment indicated that O. violaceus and C.

Western Blot分析发现野生种的FAE1都在酵母系统中获得了表达，转化诸葛菜和荠菜FAE1的酵母并未合成长链脂肪酸，而转化新疆野芥、新疆白芥和菘蓝的FAE1的酵母都有微量长链脂肪酸的合成。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

Baker's, brewer's, and Torula yeast strains serve as common substrates but in general, brewer's yeast require a pretreatment prior to use.

面包、啤酒、圆酵母可以作为原料生产酵母抽提物，但啤酒酵母抽提物要预处理后再使用。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。