酵母

France Lesaffre Group Company is one of the world famous multinational, who is founded 1853 that 150 years before and now is in the leadership in world yeast area, the Yeast and Baking Ameliorant and Extract Yeast are the 1st in the world, and the malt is the 3rd in the world.

法国乐斯福公司是世界上著名的跨国公司之一，创建于1853年，在食品酵母领域处于世界领先地位，乐斯福集团酵母居世界前列，烘焙改良剂居世界前列，酵母精居世界前列，麦芽居世界前列。

Yeast extracts are produced by a process called autolysis, which is essentially a self-digestion of the yeast, which requires the mediation of several endogenous hydrolytic enzymes.

酵母抽提物是采用自溶工艺生产的，这是酵母自我消化的过程，它要求几种酵母内源水解酶进行作用。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Sour mashes are fermented with both fresh and previously fermented yeast; sweet mashes employ only fresh yeast.

酸性糖化醪，是用酵母发酵的，这种酵母包含一部分先前发酵过的酵母；糖化醪则是用鲜酵母制得。

SUC2 mainly encodes sucrose invertase. The invertase could hydrolyse sucrose into glucose and fructose which are carbon source for yeast growth. When sucrose is used as the main carbon source, invertase is necessary for the growth of yeast. Accordingly, it is of great significance for yeast glycometabolism to study the sequence, transcription and regulation of sucrose invertase.

蔗糖转换酶基因(SUC2)编码蔗糖转换酶，通过降解酵母细胞外的蔗糖成果糖和葡萄糖来提供酵母生长所需的碳源，在以蔗糖为主要碳源的培养条件下，蔗糖转换酶对于酵母生长必不可少，因此研究蔗糖转换酶基因序列、转录、调控等对酵母蔗糖代谢具有重要意义。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Sour mash es are fermented with both fresh and previously fermented yeast; sweet mash es employ only fresh yeast.

酸性糖化醪，是用酵母发酵的，这种酵母包含一部分先前发酵过的酵母；糖化醪则是用鲜酵母制得。

The pH, acetic acid, propanoic acid and VFAs were detected, in Selenomonas ruminantium culture fluid at Oh, 6h, 12 h, 24 h, 36 h, after adding yeast. The results indicated that lactate uptake can be stimulated by all of the six yeast strains at all of six concentrations levels.

实验设6个酵母组和一个未添加酵母的对照组，为了检测酵母的最适添加浓度和有效作用时间，将每一个酵母组均设6个浓度水平：2g／L，4g／L，6g／L，8g／L，10g／L，12g／L，并且在实验的0h、6h、12h、24h、36h分别取样，检测培养液的pH值和乙酸、丙酸等挥发性脂肪酸的含量。

In order to separate good yellow wine yeast strain,the yeast in Xiangxue wine is refreshed and TTC is cultivated,and the separated yeasts undergo a whole process as the followings such as yeast water test,Erlenmeyer flask fermentation,the production of fast alcohol-and-temperature-proof yeast,and the mimic rotting vat fermentation with Erlenmeyer flask etc.

为了分离出优良的黄酒酵母菌种，通过对香雪酒中的酵母复壮和TTC选育，将分离出的酵母经酵母水试验、锥型瓶发酵、耐酒精、耐温、速酿酒母制作和锥型瓶模拟大缸发酵试验等，可使黄酒生产企业根据自己产品的类型特点和消费者的口味需求，选育出产生不同口感的黄酒的菌株来生产不同类型的黄酒。

In 2004, the company invested tens of millions of dollars to form a wholly-owned "Shanghai Jarrow Biological Technology Co., Ltd.", dedicated to beer yeast functional food research and development, the use of independent innovation in 2005, patented technology for the industrialization of production, has been developed three categories of products: the use of high-quality sugar nutrients Shu,"Shu sugar yeast", with nutrition fitness function "nutritional yeast protein powder" with Shu liquor Shugan function "Shu wine yeast."

2004年起，公司投入千万元全资组建&上海杰诺生物科技有限公司&，致力于啤酒酵母功能食品的研究和开发，2005年利用自主创新的专利技术进行产业化的生产，至今已开发出三大类产品：利用优质营养素舒糖的&舒糖酵母&，具有营养健身功能的&营养酵母蛋白粉&，具有舒肝舒酒功能的&舒酒酵母&。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。