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ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39%,38%和41%;与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50%,48%和59%。cDNA编码区序列被克隆进原核表达载体pET-30a,并在大肠杆菌BL21(DE3)中诱导表达,但过量表达的蛋白主要是以不溶性蛋白形式存在。

On the basis of regeneration system of cotyledonary nodes, using Agrobacterium tumefaciens, the Mn-SOD gene was introduced into cucumber plants to express excessively, to improve ability of cleaning ROS and stress tolerance on environmental stress. The new Mn-SOD transgenic materials could be used for study of resistance physiology.

本研究在优化黄瓜再生体系的基础上,通过根癌农杆菌介导法,将Mn-SOD基因转入黄瓜植株,使SOD在植株体内过量表达,提高其在环境胁迫下清除活性氧的能力,从而提高黄瓜植株的抗逆性,并为抗性生理的研究提供有价值的材料。

Objective:To detect the rat model of non-alcohol fatty liver disease the effect of medical intervention Methods: The Wistar rats were fed with high fatty chow for 12 weeks to induce NAFLD.And then,these rats were fed the low fatty chow,and separately intervened by Rhizoma polygoni cuspidate,Polyene phosphatidylcholine and metformin hydrychloride or controlled by distill water for 4 weeks.The total RNA of adipose tissue was extracted and reversely translated into cDNA.

Non-alcohol fatty liver disease;Intervention;Rhizoma polygoni cuspidatei;Polyene phosphatidylcholine;Metformin hydrychloride非酒精性脂肪肝病(Non-alcohol fatty liver disease,NAFLD)是无过量饮酒史的肝实质细胞脂肪积贮和变性的病理综合症,是由于肝脏本身和各种肝外因素引起的肝脏脂肪代谢功能障碍,导致肝细胞内脂类物质蓄积过多的一种病理变化,常见原因有营养缺乏、肥胖、糖尿病、妊娠、糖皮质激素、肝炎及药物或毒物的损伤等[1],发病机理尚未完全清楚。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族,它编码的蛋白质在突变体内过量表达,并造成植株矮化,莲座叶片向下卷曲,侧根发生受到抑制等形态表型的改变,同时,ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程,并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

There has been a research indicating that geminin overexpression or cdt1 knockdown decreased the number of mitotically active cells and this effect depended on direct interaction between Geminin and Cdt1 .This balanced interaction of Geminin and Cdt1 is critical for the cell-cycle regulation.

在斑马鱼中研究发现,过量表达Geminin或者降低Gdt1的表达能减少有丝分裂的细胞数量,并且这种对细胞周期的效应直接取决于Geminin-Cdt1的相互作用,总之,Geminin和Cdt1的相互作用对细胞周期的调节非常重要。

High alcohol intake has been consistently linked to breast cancer risk, but when it comes to other facets of the diet, studies have yielded conflicting results, according to the researchers on the current work, led by Dr. Valeria Edefonti of the University of Milan.

目前已经证实过量引用酒精与乳腺癌的发病风险相关,但食物中的其它成分是否与癌的发病相关,米兰大学的Valeria Edefonti教授认为,目前关于这个问题的研究结果尚不一致。

These results suggest that nimodipine in addition to its cerebral vasodilating effect may protect brain from ischemic neuronal damage through modifying iNOS expression in the infarct area, and imply that 〓 may be involved in the iNOS expression in ischemic brain tissue which contributes to the recruitment of penumbra into infarction after transient focal cerebral ischemia.

上述结果表明,除了扩张脑血管外,尼莫地平可能还通过抑制iNOS在缺血性脑组织的表达阻止NO过量产生而起到脑缺血损伤的保护作用。鉴于尼莫地平是典型的钙拮抗剂,本结果还提示〓可能涉及缺血脑组织的iNOS表达,从而介入半暗区向梗死区转变的病理过程。

According to Dr. Abramov, possible explanations for the lack of benefit include the limited pain intensity with transvaginal pelvic surgery; the short duration of preemptive analgesia (no boluses were given postoperatively); and the chance that the effect was masked by patient-controlled analgesia overdose, which he said was unlikely.

根据Abramov博士的说法,在阴道骨盆重建手术时,先进行会阴神经阻断麻醉,对於疼痛强度方面的助益不大,可能归因於阴道骨盆手术所产生的疼痛是有限的,预先麻醉期间较短;至於其效果可能被过量之管制性麻醉药给掩盖过的说法,Abramov博士表示不太可能。

First of all, the relationship between concrete strength、 acidified depth and the solution density of SO_4~(2-)、H~+, marinating period and concrete initial strength before marinating is established with dimension analysis method.

首先,本文通过量纲分析的方法确定了混凝土的强度和酸化深度与浸泡液的SO_4~(2-)离子浓度、H~+离子浓度、浸泡时间和混凝土初始强度之间的含有待拟和系数的函数关系式。

The interference behaviour of various foreign ions was tested. This method has been applied to analyze ascorbic acid in honey, cucumber and tomato samato samples and tablets of medicine.

结果表明,在0.25mol/L的硫酸介质中,在过量碘化钾的存在下,抗坏血酸的含量与到达滴定终点的时间在较宽的浓度范围内呈良好的线性关系。

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I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援,我们正徵召国家所有各种的力量:我们的外交及发展,我们的经济力量与道德劝说,所以你们与其他军人不须要孤独地负起国家安全的责任。

Imagine yourself to be an actor in a play on the stage.

设想你自己是一个演员在舞台上表演。