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General symptoms such as purpure in mucous membrance and skin, bleeding of internal organs, decreasing of weight and bad mental state caused by modeling were well improved in four control groups (Chinese traditional medicine group, western medicine group, PG original formula group and PG major formula group), the peripheral PLT was increased, spleen weight and its action of phagocytosis and damage to PLT were both decreased, morrow megakaryocytes differentiation and maturation were improved and hyperplasia descended, which showed significant differences compared with the model group. Differences among the four control groups were not significant. The optimal dose ratio of PG major formula was finded out with homogeneous design software UST3.0 and multiple regression: Astragalus root 48g, Rhubarb 15g, peach kernel 1Og, Agrimony 24g.

结果:模型建立成功;中、西药对照组、紫癜颗粒核心方组与原方组小鼠对于免疫法造模所引起的皮肤粘膜紫癜、内脏出血、体重减轻、精神状态差等一般症状均有较好的改善;小鼠的外周血小板计数得到提高;脾脏均重及对血小板的吞噬破坏降低;骨髓巨核细胞分化成熟程度增高,增生活跃度降低,与模型组相比较,均有显著性差异,四个对照组之间无显著性差异,利用均匀设计UST3.0统计软件分析,进行多元逐步回归处理,筛选出紫癜颗粒核心方的最佳剂量配比为黄芪48g,大黄15g,桃仁10g,仙鹤草24g。

Effect of nitrous oxide and volatile anaesthetics on platelet function in man.

血小板在外科手术的凝血机制中起着重要的作用,很多麻醉药会影响血小板的功能。

In addition,platelet anisocytosis and giant platelets were seen.

而且,血小板生长异质性和巨大血小板也可见到。

Results True positive rate: immature granulocyte, hemoglobin concentration variation, microcyte, left shift, macrocyte, hypochromic erythrocyte, large platelet, blasts, anisocytosis 86.7%-100.0%, hyperchromic erythrocyte 69.2%, nucleated red blood cell, atypical lymphocyte, platelet clumps

结果 真阳性率:未成熟粒细胞、血红蛋白浓度不等、小红细胞、核左移、大红细胞、低色素性红细胞、大血小板、原幼细胞、红细胞大小不等为86.7%~100.0%,高色素性红细胞为69.2%,有核红细胞、变异淋巴细胞、血小板凝集为13.3%~32.3%;以上所有参数真阴性率为82.8%~100.0%(平均95.0%)。

Objective To assess the influence of apheresis and wash on platelet function and morphology.Washed platelet of quality and clinical effect were observed.

目的 对比研究手工洗涤后血小板功能与形态的影响,观察洗涤血小板的质量和临床疗效。

Methods The apheresis platelets were transfused to 118 patients.

观察118例患者的血小板输注后的疗效,同时检测输注前后的血小板计数。

Methods The apheresis platelets were transfused to 118 patients. Peripheral blood platelet count was made after platelet transfusion.

观察118例患者的血小板输注后的疗效,同时检测输注前后的血小板计数。

Results: Volunteer blood donors were satisfied in the platelet collection procedures, and were been the fixed and voluntary apheresis platelet donors.

结果:无偿献血者对机采血小板捐献过程满意,成为固定的、志愿的无偿机采血小板献血者。

Objective: The aim of this study was to observe the protective function of trehalose and phosphate at -80℃on human apheresis platelets, and to realize the long-term(3 months) protective function of this platelet storage solution.

目的观察-80℃条件下海藻糖联合磷酸盐对人单采血小板的保护作用,并了解海藻糖联合磷酸盐对血小板的长期保存(3个月)效果。

The highly abundant platelet transcripts found in the present study included 82% or 70% of platelet-abundant genes identified in 2 previous gene expression studies on nonamplified mRNA from pooled or apheresis samples, respectively.

在本研究中发现的高度丰富的血小板纪录包括82%或70%在2项早先基因表达研究中辨认出的血小板丰富的基因,分别从合并的或分离的样品中研究未增强的 mRNA而来。

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