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In addition, the role of the RER in protein body forming, the role of amyloplast envelope in amyloplast proliferating, the relations between nucleus and cytoplasm of starchy endosperm cells in rice, and the variation of nucleus in plant PCD, the roles of plasmalemma invagination, ER, Ca〓 and phosphatases during endosperm development were discussed.

本文对粗面内质网在蛋白体形成中的作用,淀粉质体被膜在淀粉质体增殖中的作用,淀粉胚乳细胞的核质关系,植物PCD中细胞核的变化,以及质膜内陷、内质网、Ca〓和磷酸水解酶在胚乳发育中的作用等进行了讨论。

These results suggested that many organella could all develop into amyloplast, variation of amyloplast envelope resulted in amylopast proliferation, RER played an important role in synthesis and deposition of protein, ER showed many functions during endosperm development, intercellular space and plasmalemma invagination played an important role in transportation of nutrients, degeneration of starchy endosperm nucleus was a special PCD, Ca〓 and phosphatases played an important role during endosperm development.

上述结果表明:多种细胞器可以趋同发育成淀粉质体,淀粉质体增殖来源于其被膜的活动,粗面内质网在蛋白质的合成与积累中具有重要作用,内质网在胚乳发育中具有多种功能,胞间隙和质膜内陷在营养物质的运输中具有重要作用,淀粉胚乳细胞核的衰退是一种特殊形式的编程性死亡,Ca〓和磷酸水解酶在胚乳发育中起重要作用。

We reported 5 cases with intra-articular synovial hemangioma of the knee in order to improve diagnosis and treatment of intra-arfieular synovial hemangioma of the knee.

目的报告5例膝关节内滑膜血管瘤病例,以提高对膝关节内滑膜血管瘤的诊断、治疗水平。

Results: IVUS revealed that the cross-section views of coronary artery IMH appeared as a homogeneous, hyperechoic, crescent-shaped or circular area within media or between media or EEM, and the long axis views of IMH presented unbent or tortuous homogeneous space-occupying lesions with varied length.

结果:IVUS显示不冠状动脉壁内血肿的短轴观呈新月形或环形,呈等于或稍强于血液的斑点状回声,长轴观显示为中膜内或中膜与外膜之间长度不等的占位,形态或直或扭曲,呈接近血液回声或稍强回声。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

As an immunomodulator NO may play a role like a double-edged sword since it expresses both in immuonchalleged and immunosspresed states. 2. After received external stimulus the morphology of ependyma of SFO could be changed and varied with time course, particularly its cellular apoptosis do occur and earlier than the ordinary areas of the brain. 3. Astrocytes in SFO could express cFos protein induced by LPS, suggesting the cells may maintain homeostasis in the brain in a longer period and modulate immune state of the brain. 4. As a CVO, SFO may sense changes of blood-bearing immunosignals earlier and instantly response to them, thus as one of windows of the brain SFO do take part in immunomodulation within the brain.

通过我们的实验结果我们可以得出这样一些初步的结论:1、一氧化氮作为免疫调质参与了机体免疫,它既能在免疫激发状态下又可在免疫抑制状态下进行表达提示,NO在脑内免疫调节中的作用可能是双向的。2、穹窿下器的室管膜形态在接受外界刺激后,随着时间的变化而不同,扫描电镜下的凋亡形态虽需进一步证实,但室管膜细胞确实发生了凋亡,并且穹窿下器发生的较早。3、穹窿下器内的星形胶质细胞在给予大肠杆菌后确实可以表达c-fos蛋白,并可能通过自身的激活来较长时间的维持体液的动态平衡,调节脑内免疫状态。4、穹窿下器作为感受性室周器官之一,能够较早的感受血携免疫信息的改变并及时作出反应,可能作为外周免疫信息进入脑内的窗口之一来参与脑的免疫调节。

It was achieved by intradural microanastomosis of the L6 ventral root to S2 ventral root. After axonal regeneration, the "patellar ligament-spinal cord center-bladder" reflex pathway was reestablished. The function of the reflex arc was observed by nerve electrophysiological experiment, detrusor electromyography experiment, urodynamic testing and HRP retrograde tracing before and in 8 months after the spinal cord was destroyed horizontally between S1 and S4 level. There was one case of conical SCI patient who had L1 fracture and paraplegia was received intradural microanastomosis of the L6 ventral root to S2 ventral root after Li fracture and paraplegia.

将Beagle犬L6前根近端与S2前根远端在硬膜内显微吻合,经一段时间的轴突再生后,建立&膝腱-脊髓中枢-膀胱&人工反射弧,术后8个月,在破坏S1~S4脊髓节段前后分别进行神经电生理、膀胱肌电图及尿流动力学和辣根过氧化物酶逆行示踪等检查;对1例L1压缩性骨折伴截瘫患者行右侧T11与52前根经腓肠神经移植硬膜内吻合术。

Materials and Methods: Of 62 patients with spinal AVMs which confirmed by MRI, DSA and/ or pathology, 30cases had intramedullary AVMs(13 occult AVMs, 5 glopular AVMs, and 12 juvenile AVMs), 24 cases had perimedullary AVF (5 type I , 12 type Ⅱ, and 7 type Ⅲ), and 8 cases had dural AVF.

材料和方法:收集了62例经MRI、DSA和手术病理证实的髓血管畸形患者,其中髓内隐匿型动静脉畸形13例、球形AVMs 5例、幼稚型AVMs 12例:髓周膜内动静脉瘘I型5例、Ⅱ型12例、Ⅲ型7例;硬膜型AVF8例。

Results 5 cases of ependymocytoma, 3 cases of astrocytoma, 1 medulloblastoma hemangioblastoma in 10 cases of intra-medullary tumor; 36 cases of neurogenic tumor, 11 cases of spinal meningioma in 47 cases of subdural extramedullary tumor; 2 cases of neurogenic tumor strided over scleromeninx;2 cases of extradural metastatic tumor, 1 cestodiasis, 2 cases of epidural hematoma, which were mistaken for hemangioma before surgical operation and 1 vascular malformation in 4 cases of intraspinal nontumorous occupying lesion.

结果:10例髓内肿瘤中,室管膜瘤5例;星形细胞瘤3例;血管母细胞瘤1例;成神经管细胞瘤1例。47例脊髓外硬膜下肿瘤中,神经源性肿瘤36例;脊膜瘤11例。2例跨硬膜内外生长的神经源性肿瘤2例。硬膜外的转移瘤2例。4例椎管内非肿瘤性占位性病变中,椎管内绦虫病1例;硬膜外血肿2例,术前误为血管瘤;血管畸形1例。

After bleeding sloped, the hemoglobin amount in films or gauzes were measured to determine the hemostatic effect Using a randomized design, one incision in each animal was treated with C-CM membrane, the influence of C-CM film on the growth of bacteria (Staphylococcus aureus ATCC 27853. Escherichia coli ATCC 25922. Monil lia albicans ATCC 14053)were observed in vitro with solid medium. Rusults: It was observed that C-CM membrane had no toxic effects on surrounding tissue and was slowly degraded after implanted into rat leg muscular porch ; The results of hemostatic experiment showed that C-CM film adhered well to incisions.

结果:C-CM膜植入动物肌肉中,周围组织无明显炎症,未发现组织变性和坏死,可在组织内缓慢降解,具有良好的组织相容性,6周后反应接近外科缝合丝线组;止血实验结果发现:C-CM膜组和纱布组的出血时间分别为83.5±9.333s和117±15.9275s,血红蛋白光度吸收值为0.9204±0.7559和1.9764±0.7205,二组比较差异显著(P<0.05),说明C-CM膜有一定的止血作用;促进创面愈合实验结果显示:不同时间段C-CM膜组肉芽组织较对照组厚,其中胶原纤维含量亦较多,C-CM膜组的创面愈合时间早于空白对照组,伤口愈合较快;抑菌实验显示C-CM膜对三种菌产生接触性抑制。

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