膜内的

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Membrane affinity quantified by IAM and liposome/buffer systems was a more efficient predicator of α values than hydrophobicity from n-octanol/buffer system. Consequently, not only hydrophobic force but also attractive polar extra-interactions involved in the binding to IAM and liposomal membranes but not in an n-octanol phase, ascribed to the binding process within AM. The binding to ordered lipid membrane in the larger part determined drugs'binding to AM intracellular compositions, and the ordered phospholipid plasma membrane was a potential site for drugs'binding in AM.

脂质体／水系统和磷脂膜色谱所测定的膜亲和性比正辛醇／水系统的疏水性更好的预测巨噬细胞内药物的α值，因此包含在磷脂膜色谱中但并不体现在正辛醇／水系统中药物与磷脂膜之间的额外吸引性极性作用力对药物在细胞内结合有着重要贡献；药物与有序磷脂膜的结合在很大程度上决定着药物与巨噬细胞内成分的结合，有序磷脂分子膜可能是巨噬细胞内部潜在的结合部位。

There are two types of maturation according to our observation.One is nucleocapsids obtained its tegument in the nucleus and enveloped from the inner nuclear membrane.Another is nucleocapsids entered cytoplasm through nuclear membrane,then obtained their tegument in the cytoplasm,enveloped from the plasma membrane,finally released by necrosis,exocytosis or other ways.

病毒成熟有两种方式：一为细胞核内核衣壳在核内获得皮层，通过核内膜获得囊膜成为成熟病毒；二为核内核衣壳通过内外核膜进入胞浆，核内和胞浆内的核衣壳在细胞浆中获得皮层，然后在各种质膜上获得囊膜，最后成熟病毒通过细胞破裂或其他方式释放到细胞外。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

Type'.'' genus of the family Polyangiaceae: myxobacteria with rounded fruiting bodies enclosed in a ''.

多囊粘菌科的一个模式属；含有粘细菌带有包在一层膜内的圆形果质体。

By using red blood cell broken-up solution,proteinase K and RNase,total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species,Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.

本实验以河南董寨自然保护区白冠长尾雉和环颈雉已孵化的卵壳为材料，利用红细胞破碎液、蛋白酶K及RNA酶等试剂，对卵壳膜内的总DNA进行了提取，建立了一种提取高质量DNA的新方法。

Results: Before the fixed bridge was applied, under vertical load, the high-stress region was near around the apical of periodontal ligament with mainly compressive stresses. Under oblique load, the high-stress region was near around the cervical of periodontal ligament with both tensile and compressive stresses. After the fixed prosthodontics, stress and strain distributions were similar with that when the fixed bridge was not applied. But the maximum stress, strain and displacement increased apparently. The stresses in exterior surface of periodontal ligament were larger than that of interior surface; this character of periodontal ligament under vertical load was more obvious than that under oblique load.

结果：固定桥修复前，垂直载荷下，基牙牙周膜的应力以压应力为主，应力集中在根尖；斜向载荷下，基牙牙周膜的应力主要是拉应力和压应力，应力集中在唇、舌颈缘；固定桥修复后，基牙牙周膜的应力和应变分布规律与修复前相似，但最大应力、应变值都明显增大；牙周膜内表面的应力大于牙周膜外表面；垂直载荷时，牙周膜内、外表面的应力差异更为显著。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups（P＜0.01）.The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group（P＜0.01,P＜0.05）.The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面，差异均有显著性（P＜0.01）；IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性（P＜0.01）； MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达，差异具有显著性（P＜0.01，P＜0.05），MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达；TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。