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The experiment shows that human pancreatic cancer cell line Patu 8988 has GGT→GTT mutation at codon 12. The method of PCR-SSP is rapid, convenient, economical and high specific for detecting K-ras gene point mutation. The goal in the long term is to develop a simple and useful clinical procedure for early diagnosis and differential diagnosis of pancreatic cancer.

明确了人胰腺癌细胞株Patu 8988 K-ras基因第12位密码子点突变方式,为胰腺癌的下一步基因治疗研究打下了坚实的基础;PCR-SSP方法检测K-ras基因点突变简便、快速、经济且特异性高,有望在临床上成为早期诊断和鉴别诊断胰腺癌的实用检测方法。

Objective To evaluate the visualization of the abnormal small peripancreatic vessels in patients with pancreatic carcinoma using dual phase spiral CT and the value of peripancreatic vessels SCTA.

目的 探讨胰腺肿瘤时胰周小血管的异常改变和胰周血管SCTA临床价值。方法 2 0例胰腺肿瘤作了一次性屏气螺旋CT双期增强扫描,横断面观测胰周小血管的直径,并用动脉期扫描图像作SCTA ,进一步显示肿瘤对胰周血管和胰腺侵犯的空间解剖关系。

The method is an effective way to increase the pharmic concentration in the pancreatic blood,and it can also decrease the morbidity and mortality of the patients with acute severe pancreatitis.Thus,this treatment method might be recommendˉed in our clinical practice.

手术中置管并在术后持续药物灌注是治疗急性重症胰腺炎的有效方法,它能有效地提高胰腺局部的血药浓度,选择性强,能有效减少胰腺炎的并发症和死亡率,是一种值得进一步探讨和推荐的治疗方法。

The method is an effective way to increase the pharmic concentration in the pancreatic blood,and it can also decrease the morbidity and mortality of the patients with acute severe pancreatitis.Thus,this treatment method might be recommendˉed in our clinical practice.

手术中置管并在术后持续药物灌注是治疗急性重症胰腺炎的一种有效方法,它能有效地提高胰腺局部的血药浓度,选择性强,能有效减少胰腺炎的并发症和死亡率,是一种值得进一步探讨和推荐的治疗方法。

Objective To evaluate the effect of different procedures in the treatment of pancreatic pseudocyst.

胰腺假性囊肿是最常见的一种胰腺囊性病变,约占全部胰腺囊性病变的 75%。

The choice of surgical procedure of pancreatic trauma should be made on the basis of the pancreatic trauma.Drain should be trough free and strengthen trophotherapy after operation

结论胰腺损伤早期,术前诊断困难,对怀疑有胰腺损伤者,应尽早手术探查,并根据胰腺损伤情况选择合理的手术方式,引流要通畅,术后加强营养支持。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量,结果表明,虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin,白色闭壳肌中未检测到类胡萝卜素,其中肝胰腺除上述两种类胡萝卜素外,还含有其他多种未鉴定的类胡萝卜素;在各组织中,雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高;橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝;雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察,并未观察到异常现象;超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌,在橘红色闭壳肌中有三种肌纤维,其中疏松型肌纤维为橘红色闭壳肌特有。

cDNA microarray was an effective and useful method to survey gene expression profiles in pancreatic carcinoma. MBD1、EDG1 and its hyper methylating modulation may play an important role in the pathogenesis of pancreatic cancer, among which the high expression of MBD1 in pancreatic cancer would be the key modulating point in the down regulation of multiple methylation related suppression genes transcription.

cDNA微列阵技术为胰腺癌相关基因表达谱的分析提供了有力的工具,MBD1、EDG1等基因过甲基化表达调控可能在胰腺癌发病机制中起着关键作用,而MBD1在胰腺癌中高表达可能是多个甲基化相关抑癌基因转录表达下降的关键调控点。

MBD1、EDG1 and its hyper methylating modulation may play an important role in the pathogenesis of pancreatic cancer,among which the high expression of MBD1 in pancreatic cancer would be the key modulating point in the down regulation of multiple methylation related suppression genes transcription.

cDNA 微列阵技术为胰腺癌相关基因表达谱的分析提供了有力的工具,MBD1、EDG1等基因过甲基化表达调控可能在胰腺癌发病机制中起着关键作用,而MBD1在胰腺癌中高表达可能是多个甲基化相关抑癌基因转录表达下降的关键调控点。

Among these results,expression patterns of 21 genes were consistent with those in the microarray data.3.3 Emodin potentiates growth inhibition induced by gemeitabine in pancreatic cancer cells in vitroTo investigate the effect of ernodinon cell viability,pancreatic cancer cell lines (Pane-1,Mia Paca-2 and Bxpc-3) were treated with increasing concentrations of emodin (0-160μM) for 24,48 and 72 hours.

结果显示21个基因两种方法所得基因表达倍数大致相当。3.3 Emodin在体外增加吉西他滨对胰腺癌细胞生长的抑制为了探讨Emodin对胰腺癌细胞生长的影响,应用MTT方法检测不同浓度的Emodin(0-160μM)作用于胰腺癌细胞24h、48h和72h的效应。

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