结构基因

Rapid alkalinization factors were polypeptide signals in the plant, belonging to a large gene family. The activity of RALF could induce extra-cellular pH increasing rapidly. Many studies on RALFs were in Solanum, including the structure and maturation, the gene expression, receptors and functions and so on.

快速碱化因子类基因属于植物多肽类信号分子，是一类大的家族基因，它的活动引起胞外pH值快速升高，有关RALF类基因的研究在几种茄科植物中开展的较多，包括RALF蛋白信号分子的结构及加工、基因表达、受体研究以及功能等方面。

Cloning and Expression of a clNA Encoding a Snake VenomMetalloproteinaseAbstract: A cDNA clone of 987bp is amplified from the total RNA of Agkistrodon halys Pallas snake venom gland by RT-PCR. Deduced amino acid sequence indicates that HXL is composed of a partial open reading frame encoding 317 amino acid residues.

从江浙蝮蛇的毒腺中提取总RNA，经RT-PCR扩增出一个长为987bp的金属蛋白酶基因，该基因编码317个氨基酸的开放性阅读框，包括酶原前肽结构域的一部分，完整的金属蛋白酶结构域和去整合蛋白结构域。

In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断，同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系，比较GenBank中MDPV和GPV的基因序列，针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段，分别构建两对PCR引物。第一对引物LHMP1／LHMP2是根据MDPV的基因序列设计的，上游引物针对MDPV基因序列的454～472；下游引物针对基因序列的1173～1155，为19个碱基，扩增跨幅为719bp。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

Computer was used to analyze the possible secondary cleavage sites on HPV11 E2 mRNA and to predict the secondary structures of substrate and ribozymes. According to the theory of Symons headhammer ribozyme, there are 32 sites to targetting sequences.

遵循Symons锤头状核酶结构和GUX剪切位点原则，靶序列存在32个剪切位点，通过计算机软件分析核酶的最佳剪切位点，并对底物及核酶的二级结构进行预测及进行相应基因生物学功能和基因同源性分析，筛选出2个锤头结构核酶。

In the present study C-mos nuclear and 12S rRNA mitochondrial DNA were employed as genetic markers to deduce the interrelationships among Gekkonidae lizards; Cyt b and ND2- tRNA~ gene sequences were used to discuss the population structure of Hemiphyllodactylus yunnanemis; 12S rRNA gene sequences were used to reveal the phylogenetic relationships among seven species of the genus Gekko.

基于12S rRNA基因全序列及C-mos基因序列片段探讨了壁虎科蜥蜴的属间关系；用Cyt b基因全序列和ND2-tRNA~基因片段初步研究了云南半叶趾虎的种群结构；用12S rRNA基因全序列探讨了中国壁虎属七物种间的系统关系。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知：大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427)，新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀，经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现：全长分别为16 523bp和16549bp，均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区，紫貂全序列中碱基组成为A-32.0%，C-27.6%，G-14.7%，T-25.8%，黄喉貂为A-32.5%，C-26.9%，G-14.1%，T-26.5%；基因排列顺序与日本貂和貂熊的一致，但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

Therefore, we selected the 5 genes, as well as SMTN gene related to human cardiovascular diseases, for chromosomal mapping, CDS cloning and analysis, spatio-temporal distribution, mutation riddling, and association analysis with production traits of 3 porcine populations. We expect to know the structure and function of these 6 genes primarily, and supply data for porcine marker assistant selection of improving meat production.

因此，本研究选择NADH呼吸链相关基因，以及与人类心血管疾病相关的SMTN基因，以猪的骨骼肌为研究对象，用人×仓鼠体细胞杂种克隆板进行6个基因的染色体定位、CDS克隆和序列分析、用半定量RT-PCR进行时空表达谱分析、进行突变位点的筛选、并利用3个猪群的产肉性状资料进行基因型与性状的关联分析，以期初步了解这些基因的结构和功能，并为改进猪产肉性能的标记辅助选择提供资料。

Bioinformatics was used to seek the homologous gene of EXT1 in Strongylocentrotus purpuratus which was just discovered and had the correlation to human hereditary multiple exostoses. The sequence, exons, coding protein, physical and chemical characters of EXT1 gene were analyzed. The structure and function of its coding protein were predicted, and the phylogenetic tree for the homologous gene was constructed, which provided certain basis for the future research of human EXT1 gene.

利用生物信息学方法寻找人类遗传多发性外生性骨疣EXT1基因在紫色球海胆中的同源基因，对该基因的序列、外显子信息、编码蛋白及其理化性质进行分析，并预测其编码蛋白的结构与功能，构建其同源基因的系统进化树，旨在为进一步研究人体EXT1基因提供一定的依据。

The results of searching human genome indicate that there may be two more homolog genes respect to hPDIPl, the case is similar in the mouse genome. The homologies encode proteins of conservative amino acid sequence, each has a BTB/POZ domain in the N"-terminal and conserved PCNA-binding motif in the C"-terminal. Thus we rank them PDIPl gene family.

我们在GeneBank中搜索到在人类有两个与人类PDIP1基因具有较高同源性的基因，同样在GeneBank中也搜索到了在小鼠也有两个与小鼠PDIP1基因同源性较高的两个基因，它们在氨基酸序列高度保守，与PDIP1基因一样，在N端区域，都有一个BTB/POZ的结构域；而在C端区域，则是PCNA的结合基序。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。