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However, chloroplast matK genes are indispensable since in nonphotosynthetic parasitic plant, Epifagus virginiana and fren, Adiantum capillus-veneris which chloroplast genome has rearrangememt, the chloroplast matK is functional even being a free-standing from with dismissed trnK exons.The chloroplasts of Psilotum, moss and liverworts all have trnK5'-matK-trnK3' structure, but it is found that matK is a pseudogene in hornwort Anthoceros formosae. Little is known in other lower land plants, it is the purpose of this thesis to examine the presence of chloroplast matK gene is these taxa.

叶绿体trnK5'-matK-trnK3'的结构在高等植物相当一致,但先前研究发现两个matK脱离trnK而存在的例子,像是叶绿体严重退化的寄生植物Epifagus virginiana及因叶绿体基因重组而导致trnK外显子丢失的铁线蕨,它们的matK仍然具有完整的开读框,显示了matK的确十分重要而不可缺失,藓苔类和松叶蕨也被证实具有trnK5'-matK-trnK3'的结构,但是在角藓中却发现其matK是伪基因。

There were 10 genes which were preferentially expressed in fibers, and transcripts of the other genes were dominantly accumulated in petals, anthers or leaves etc., respectively.

对所分离的基因及其编码的蛋白质结构进行了比较分析,以期阐明它们之间的进化关系以及基因和蛋白质结构与功能表达之间的相关性。

Comparing with database in GenBank, the results showed RBB1 had 91% homology to mRNA for a known ATP synthase CF1 epsilon chain 89% homology to ATPase epsilon subunit in Zea mays and 87% homology to Triticum aestivum ATP synthase CF1 epsilon chain.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析,同样证明了该基因为H〓-ATP合酶CF〓亚基蛋白编码基因,其氨基酸序列3-83之间和1-133位氨基酸之间具有两个保守的蛋白结构域ATP-synt-DE-N和AtpC。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法,对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测,以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

The complex of the structure and its putative protein of this gene implies its importance in regulating the wheat development.

该基因结构及其推定产物结构的复杂性说明该基因在生命活动调节中的精确性。

The secondary structure of 5'noncodening region (5'NCR) and core region of HCVH strain as target sequences was calculated with the help of computer, the ribozymes against HCV were designed according to the"hammerhead structure"suggested by Symons.

根据锤头结构Rz的设计原理,以HCV-H株基因序列为靶,通过对靶RNA二级结构的能量分析,在HCV 5'NCR和C区选择出213、260、470和478四个理想的靶位点,设计出Rz的基因序列。

The p53 gene structural abnormality and mdm2 protein overexpression might be two factors causing p53 protein overexpresion in NPC. The function of p53 proteins produced by various structural anormality of p53 gene might be different in activating MDM2. The overexpression of p53 or mdm2 protein, particularly both cooperation, might play an important role during NPC genesis.Key words: Nasopharyngeal neoplasms; p53 gene; p53 protein; mdm2 protein

p53基因结构改变和mdm2蛋白过度表达是影响NPCp53蛋白过度表达的两个因素;不同类型p53基因结构改变所产生的p53蛋白激活MDM2的功能并不相同;p53和mdm2蛋白过度表达,尤其两蛋白协同作用,可能在NPC发生中起重要作用。

It encodes a putative zinc finger transcription factor, and is a transcriptional corepressor for thyroid hormone receptors. Hairless gene plays a critical role in maintaining the delicate balance between cell proliferation, differentiation, and apoptosis in the hair follicle as well as in the interfollicular epidermis, and is concerned with the control of hair growth cycling.

无毛基因是与皮肤和被毛结构有重要关联的核受体基因,编码一个锌指结构转录因子,是甲状腺激素受体的转录辅阻遏物,并参与毛发生长周期的调控,在维持毛囊及毛囊间上皮的增殖、分化、调亡的精致平衡中扮演重要角色。

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI (http:///www.ncbi.nlm.nih.gov/), ExPaSy (http://www.expasy.org/) and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心(NCBI,http:∥www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http:∥ca.expasy.org/)中有关基因和蛋白的序列和结构信息分析的各种工具,结合其它生物信息学分析软件包,从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后,使得对DNA的研究不断深入,但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子,还是目前最令人关注的基因工程、染色体绘制等,都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂,目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶,但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短(约为4~8个碱基对),而且一般要求回文结构,这就给飞速发展的基因工程技术带来一定的限制。

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