结构基因

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Methylation is negative correlation with genetic transcription , and the mechanism is probably as follows : directly interfere the combination of specific transcripton and recognition site of each promoter ; mediated transcription inhibition by change the construction of chromatin; induced gene silencing by combination of methylation site and transcription inhibition. P16 gene is also called multiple tumor supresser gene , which is the best easily to methylate in the anti-oncogenes. P16 gene locates in 9p21 of mankind-chromatosome , and it's total length is 8.5kb .

甲基化与基因转录呈负相关，其机制可能是[3]：直接干扰特异的转录子和各自启动子识别位点的结合；通过改变染色质结构，介导转录抑制；甲基化DNA位点直接与转录抑制子结合，诱导基因沉默。p16基因又称为多肿瘤抑制基因( multiple tumor suppressor 1 ，MTS1) [4] ，是肺癌中最易发生甲基化的抑癌基因，该基因位于人类染色体9p21上，全长8.5kb ，由两个内含子和三个外显子组成。

In the new view, viz. Neo-Darwinism theory, product family development system is analyzed and the architecture of product family gene model is built. After analyzing the gene expression of product family model, the center-principle for product family gene model is proposed and its information hereditary mechanisms are clarified. The algorithms of transcription, retrotranscription, translation, direct-translation and mutation for information gene are presented.

从一个新的角度——现代达尔文主义学说对产品族设计系统进行了分析，在此基础上，建立了产品族基因建模体系结构，分析了产品族模型的基因表达，提出了产品族基因建模中心法则，并对其中各个信息遗传机制进行了详细的分析和论述，给出了相应的信息基因转录、逆转录、基因转译、基因直译以及基因突变的算法。

The full-length cDNAs of rice and wheat S-adenosylmethionine decarboxylase gene were isolated and characterized. The rice SAMDC cDNA is 1560 bp in length, encoding a polypeptide of 398 amino acids, and the wheat SAMDC cDNA is 1351 bp in length, encoding a polypeptide of 392 amino acids. Both the rice and wheat SAMDC proteins contain the conserved proenzyme cleavage site and the PEST domains, which are commonly found in the SAMDC protein sequences of all eukaryotes.

克隆了水稻和小麦SAMDC基因的全长cDNA序列，所获得的水稻SAMDC基因序列长1560 bp，编码一个由398个氨基酸组成的多肽，小麦SAMDC基因序列长1351 bp，编码一个由392个氨基酸组成的多肽，二者均含有真核生物SAMDC蛋白所拥有的两个保守的氨基酸结构域：酶原剪切位点和PEST结构域。

This article summarizes the research achievement on the structure of anura mtDNA this year, analyzes the diversity of frogs mtDNA gene rearrangement, we found that there are 8 kinds of different mtDNA arrangement orders altogether, and also preliminarily analyzes the relation of this different gene structure.

本文综述了今年来在无尾目线粒体基因组结构的研究成果，分析了蛙类mtDNA基因重排多样性，结果发现无尾目动物共有8类不同的线粒体基因排列方式，并初步分析了这些不同基因结构的关系。

This course systematically describes the structure and function, transmission, expression and regulation, and evolution of genetic materials, covering basis of cytology for inheritance, molecular basis of genetic materials, Mendel's principles of inheritance, linkage and sex linkage, genemutation, variations in chromosome structure and number, quantitative genetics, inbreeding and heterosis, inheritance of bacteria and viruses, extranuclear inheritance, gene engineering, genomics, gene expression and regulation, developmental genetics, population genetics and evolution.

本课程全面系统地介绍遗传物质的结构与功能、遗传物质的传递、遗传物质的表达与调控、遗传物质的进化等，包括遗传的细胞学基础、遗传物质的分子基础、孟德尔遗传、连锁遗传和性连锁、基因突变、染色体结构变异、染色体数目变异、数量性状的遗传、近亲繁殖和杂种优势、细菌和病毒的遗传、细胞质遗传、基因工程、基因组学、基因表达的调控、遗传与发育、群体遗传与进化。

These results indicated that the genetic variation within exon 2 appeared to have largely arisen by gene recombination and balancing selection. Alignment of the deduced amino acid sequences of the β1 domain coded by exon 2 revealed 6 synonymous mutations and 27 nonsynonymous substitutions at the 33 disparate sites.

对B-LBⅡ等位基因第二外显子所编码的B-LBⅡ分子β1结构域氨基酸序列比对发现，31个B-LBⅡ新等位基因属于26个等位基因主型；在β1结构域氨基酸序列的33个变异位点上，存在6个同义替换和27个错义替换。

The effect of transgenx Bt cotton on non-target insects The kinds of the dominant species of pests in transgenic cotton field had obvious change, in which the H. armigera was no longer the main pest and the piercing-sucking pests such as Aphis gossypii, Tetranychus urticae, Thrips tabaci etc. rose the main pests. The kinds of the dominant species of predatory natural enemies seemed to be no variation, which was still Propylaea japonica Thunberg. Because of the effect of transgenic Bt cotton on H. armigera laevae-the development delay, the weight reduction, which affected the qualities of parasitic enemies, leading to the reduction of the weight of cocoon and the weight of adult, and the reduction of H.

转Bt基因棉对节肢动物群落整体结构及时序变化的影响 2001保铃棉与冀抗668的相似系数最高(0.8246)，冀抗668、保铃棉与常规防治的相似系数依次0.6459、0.5829，保铃棉和冀抗668与常规不防治的相似系数为0.5669、0.4485.2002 年与冀杭668的相似系数最高(0 。6887)，保铃棉和冀抗668与常规防治相似系数为0.4739、 0.3828，保铃棉和冀抗668与常规不防治的相似系数为0.3124、0.3999，此结果表明，两转Bt 基因棉田节肢动物群落的整体结构一致，相似性较大，与常规防治相比，相似系数有所降低，表明常规功治棉田中人为控制因素对节肢动物有一定影响；转Bt基因棉对节肢动物群落有一定影响，但并不显著。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。