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Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示,应用卵裂球电融合方法可以制作一系列的多倍体胚胎;小鼠囊胚的形成与胚胎中的细胞数目无直接关系,卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程;而胚胎细胞中的核/质比可能是控制胚胎囊胚化的一个重要因素。

Therefore, the oocytes quality decline induced by ooplasmic defect may be the main reason that cause older female sterility and pregnancy failure.

因此,卵胞质缺陷诱发的卵母细胞质量的下降可能是引起许多老龄妇女不育和怀孕失败的主要原因。

Ooplasmic transfer increases the fertilizability of the aged oocytes and the early embryonic viability, it has led to the birth of health babies, and become a hotspot in the realm of human assisted reproduction biotechnology and animal clone.

卵胞质转移技术能有效改善老龄卵母细胞的受精能力和早期胚胎的发育能力,在人类已有健康后代出生,它已成为人类辅助生殖生物技术和动物克隆研究的新热点。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作,建立卵胞质内圆形精细胞显微注射受精技术程序,探索猪圆形精细胞显微受精的可能性,研究影响这种特殊细胞显微注射受精效果的因素,为进一步研究猪ROSI技术提供基础资料。

Taking Phasianus colchicuses and Gallus gallus as comparisons,the contents of protein and amino acides in egg albumen, mineral in egg shell were measured by kjeldahl method,HPLC and atomic absorption method respectively.

以红腹锦鸡为研究对象,以七彩山鸡和家鸡为参照,采用凯氏定氮法和高效液相色谱法分别测定了3种鸟类卵清中蛋白质含量及17种氨基酸含量,以原子吸收光谱法测定了其卵壳中7种矿质元素的含量。

Leaves simple; petiole 1.5--4(--7) mm; leaf blade ovate or lanceolate in outline, pinnatipartite or pinnatisect, sometimes pinnatilobed, 0.5--5 × 0.3--3 cm, leathery, both surfaces sparsely puberulous or subglabrous, base cuneate or subtruncate; lobes in 1--3(or 4) pairs, undivided and linear or subrhombic and unequally 2- or 3-lobulate, 4--15 mm, apex acute; lobules ovate, triangular, or linear; midvein abaxially slightly prominent or flat, inconspicuous.

单叶 叶柄1.5-4(-7)毫米;叶片卵形或披针形轮廓,羽状深裂或羽状全裂,有时羽状浅裂的, 0.5-5 * 0.3-3 厘米,革质,两面稀疏被微柔毛的或近无毛,基部楔形或近截形;在对,不裂和线形或1-3(或4)内裂片和不相等2或3具小裂片,4-15毫米,先端锐尖;卵形,三角形的小裂片,或线形;中脉的背面的稍突出或平,不显眼。

Branchlets, petiole, and peduncle with translucent hairs; leaf blade ovate, oblong-ovate, or elliptic, submembranous or thinly papery

小枝,叶柄和有半透明的头发的花序梗;叶片卵形,长圆状卵形,或椭圆形,近膜质的或薄纸质 30 H。

Leaves alternate, long petiolate, 2-4 × ternately compound, rarely simple; stipules membranous; leaflets lanceolate, ovate, or broadly ovate to elliptic, margin dentate.

叶互生,具长叶柄, 2-4 *三出的化合物,很少单;托叶膜质披针形,卵形的小叶,或宽卵形到椭圆形,边缘具牙齿。

Leaves simple, rarely trifoliolate, opposite, sessile or shortly petiolate, no more than 5 mm; blade ovate to oblong-ovate, 11-14(-16.5)× 5.5-7(-8) cm, coriaceous, glabrous, adaxially lustrous green, abaxially pale green, main vein impressed adaxially and prominent abaxially, lateral veins 5-9(-11) pairs, slightly discernible adaxially and conspicuous abaxially, base subcordate, rarely rounded, margin glandular crenate or -serrate, apex apiculate, cusp 5-10(-15) mm.

单叶,很少具三小叶,对生,无梗或短具叶柄,顶多5毫米;叶片卵形到长圆状卵形, 11-14(-16.5)* 5.5-7(-8)厘米,革质的,无毛,正面的是有光泽的绿色,背面的淡绿色,主脉正面凹陷和突出背面,侧脉5–9(–11)对,正面稍可见,背面明显,基部近心形,很少圆形,边缘腺状圆齿或腺锯齿,先端具细尖,尖突5–10(–15)毫米。

Artificially induced a single 〓elevation could effectively drive the activation, in recently ovulated oocytes (12—13 h after hCG) however, a single Ca〓 increase was futile in initiation of activation and only repetitive 〓 rises were avalable, suggesting that the activation of recently ovulated oocytes requires oscillatory Ca〓 signals as occuring at fertilization.

只有诱导卵内Ca〓多次升高才能使之激活。提示:刚排出不久的卵激活象受精一样需要胞质Ca〓波动信号。

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