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Result:The best prescription was : Lecithin: Sodium Cholate =8∶1,Lecithin:Proanthocyanidins =15∶1,sonde-type ultrasound time was 5 min,the entrapment efficiency of FNL was 67.13%,and the results revealed the FNLs were sphere like with the mean size of 168.3 nm,the mean Zeta potential was –86.6 mV.

结果:最佳处方和制备工艺条件为卵磷脂与胆酸钠的质量比为8∶1、卵磷脂与原花青素的质量比为15∶1、探头式超声5 min。包封率为67.13%,所得柔性纳米脂质体为类球形实体粒子,平均粒径168.3 nm,平均Zeta电位-86.6 mV。

Leaf blade membranous, oblong-ovate or ovate-orbicular, margin crenate to serrate; terminal bud not spinescent.

叶片膜质,长圆状卵形的或卵形圆形,对有锯齿的边缘具圆齿;顶芽不具刺。

It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论:(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率;(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育;(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Odd a shape compound leaf, flocculus 7-11(13), egg form or circle of regulations of state pouring egg, small change is simple, whole reason, opposite, grow 3.5-8.0cm, wide 2-5cm, tip acute is pointed, base ministry wide wedge or circle, without wool, below young leaf be grown closely fluff.

奇数羽状复叶,小叶7-11(13),卵形或倒卵状矩圆形,薄革质,全缘,对生,长3.5-8.0cm,宽2-5cm,先端锐尖,基部宽楔形或圆形,无毛,幼叶下面密被长柔毛。

As investigated, the shape of the fruit was mainly rotundity, and the color of the fruit were red, black, yellow, mauve. Variation coefficient of mineral elements, which revealed wider variation range and rich genetic diversity. Among them, Fe, the content and the variation coefficient of which was the highest(2.12 mg·100g-1 and 26.99%), it has large potential for futher selection. Ripe fruit of 12 wild myrobalan plum seedlings were analyzed using head space-solid phase microextraction and gas chromatography-mass spectrometry. The results showed considerable genetic variations in these aspects: The total content of volatile components, the classes and contents of each compounds classes, the segregation ratio, and content of main components. There were 83 compounds in total belonging to 6 classes detected in 12 wild myrobalan plum seedlings, including esters, alcohols, ketones, aldehydes, heterocycle and Hydrocarbons. Among them, Formic acid, hexyl ester, the content of which was the highest(4.33 μg·g-1),was important character impact odors.

结果表明,①新疆野生樱桃李实生株系的果实形状主要为圆形,少数为椭圆形、卵圆形、卵形和宽卵形,果实颜色有黄色、红色、紫红色和黑色等4种类型,野生樱桃李果实纵径、横径、单果重、果形指数以及可溶性固形物含量均存在一定程度的变异,遗传多样性较为丰富,其中以单果重的变异系数最大(9.13 %);②新疆野生樱桃李实生株系果肉组织的Zn、Ca、Mg、Mn、Fe及Cu等6种矿质元素含量变异丰富,其中以Fe元素的含量(平均值为2.12 mg·100g-1)和变异系数(26.99%)最高,进一步筛选高铁性状单株的潜力很大;③从新疆野生樱桃李12个实生株系中共鉴定出醇类、醛类、酯类、酮类、烃类及杂环类等6类83种挥发性化合物,各实生株系挥发性化合物总含量、挥发性化合物种类及其含量以及主要挥发性化合物分离比率与含量等存在广泛的遗传变异,遗传多样性较为丰富,其中以甲酸己酯含量最高(平均值为4.33 μg·g-1),是野生樱桃李果实香气形成的重要特征性化合物。

Spikelets laterally compressed, florets several, all bisexual or lowest sterile and without a palea; rachilla glabrous, disarticulating above glumes and sterile floret and between fertile florets; glumes lanceolate, shorter than lemmas, subequal or unequal, scarious-membranous, 1–3-veined, obtuse to acuminate or aristulate; lemmas ovate-lanceolate, scarious-membranous, 3-veined, long-ciliate on lateral veins, keeled, apex setaceously bidentate with a short, often recurved awn from the sinus; palea shorter than lemma, hyaline, keels very shortly ciliate.

小穗侧面压扁,小花数个,全部不育和没有一内稃的最低的两性的或;高于颖片和不结果的小花无毛,脱节的小穗轴和在肥沃的小花之间;披针形的颖片,短于外稃,近等长或不等长,干膜质膜质,1-3脉,具小芒的钝到渐尖或;卵状披针形,干膜质的外稃膜质,3脉,具长缘毛在侧脉上,龙骨状,先端setaceously具双齿具短,通常下弯从那里那些凹缺;内稃短于外稃,透明,龙骨非常短的纤毛。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达,由原始生殖细胞产生卵原细胞,髓质退化成为卵巢腔,分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加,它们在分化结束后解体,分化前细胞内有较多的大颗粒,在分化中大颗粒减少,小颗粒增多,在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜,髓质有许多睾丸管构成,管腔内有散落排列的精原细胞。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF,经胚胎移植后顺利产仔;在异种核移植方面,小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通过延迟生发泡破裂和促进胞质成熟的方式,使绵羊卵母细胞的核质成熟趋于同步,有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle, GV)卵母细胞;用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达:其中cDNA的合成分两种方法进行:一是将GV期单个卵母细胞直接进行RT合成cDNA,二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT;回收产物构建克隆质粒并测序。

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