卵质

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early primary oocyte, and localized in the cytoplasm and nucleoplasm of oocytes at the large growth stage and mature stage.

在小生长期，3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜；在大生长期和成熟期，则定位在胞质和核质。

Methods: Lecithin and the sucrose ester-phospholipids liposome were prepared using the ethyl ether injection method by mixing sucrose ester with lecithin, sphingomyelin and cholesterol according to different lipids ratio.

按不同比例将乙酸异丁酸蔗糖酯与卵磷脂、鞘磷脂和胆固醇混合，溶于乙醚中，然后注入pH7.2的PBS缓冲液中，制成卵磷脂脂质体及蔗糖酯－卵磷脂脂质体。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.

目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验，取50只小鼠随机分成5组，分别给予溶媒和不同剂量的色素，然后采用溴代苯致实验性肝损伤，测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用，抑制率随样品的浓度增高而增大，并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中，中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17 b -estradiol, testosterone and progesterone antibodies.

在卵巢发育早期，卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应；在大生长期和成熟期，卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱，而对雌二醇则显著增强。

Stage I of both male and female stone flounder appears only once in all its life, and the germ cells are comprised by spermatogonia or oogonia. The gonad from Mar to Aug keeps at stage II. The gonad index of testis at this stage is 0.037%, and the amount of spermatogonia is increased quickly. There is some linear germ plasm in the cytoplasm of spermatogina. In ovary at this stage it is mostly composed by oocyte of phase 2 which the character is the appearance of yolk nucleus, and no zona radiate in membrane. The mean GI of ovary is 1.95%. From Sep to Oct gonad is at stage III which testis is composed by lots of spermatogina and few spermatocytes, and the mean GI of testis at this stage is 0.086%. In ovary the ooctyes at phase 3 are in dominate position, the yolk nucleus disappear. And the GI of this stage is 3.35%. Both testis and ovary are at stage IV in Nov. hi testis the germ cells are in spermiogenesis, and the mean GI is 0.93%. hi ovary the oocytes are mostly at phase 4, which are filled in the cytoplasm with vitellin granule, and the zona radiate in membrane begins to formation. Nucleus moves to one side of the oocyte gradually. The mean GI of ovary at this stage is 9.37%.

在每年的3月-8月期间性腺处于Ⅱ期，此期精巢中精原细胞明显增多，胞质局部可见有线状的生殖质存在，平均成熟系数为0.037％；卵巢中以2时相卵母细胞为主，可见细胞质中出现强嗜碱性的卵黄核，细胞外由一层滤泡细胞包围，但尚无放射带，平均成熟系数为1.95％。9月-10月期间性腺处于Ⅲ期，此期精巢中仍有大量精原细胞，同时可见部分精母细胞，平均成熟系数为0.086％；卵巢中以3时石鲜孟加限加$玩印面n洲匆s性腺发生、分化及发育的周年变化相卵母细胞为主，细胞质中的卵黄核己消失，平均成熟系数为3.35%。11月性腺处于IV期，此期精巢内精细胞正处于不同的形成过程中，平均成熟系数为0.93%；卵巢中以4时相卵母细胞为主，胞质中充满染成桔红色的卵黄颗粒。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。