卵质

In coalfield exploration in Runan County of Henan Province,thickness of tertiary and quaternary formation is up to 700 m,water sensitive clay,sandy clay,sandy argillite,aleurolite and gravel strata are often encountered.These easily cause hole diameter's shortage or enlargement,wall of hole collapsing and breaking.

在河南省驻马店汝南县林楼一正阳县张楼一带的煤田勘探中，第三、第四系地层厚达700m，经常遇到水敏性粘土、砂质粘土、砂质泥岩、粉砂岩和卵砾石等地层，易造成缩径、超径、坍塌和掉块等孔内复杂情况。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

Stamens not less than 10; leaf blade broadly ovate to cordate or orbicular, membranous to subleathery

不少于10的雄蕊；叶片宽卵形到心形或圆形，膜质到近革质 2

Leaf blade usually lanceolate to ovate, rarely linear or subulate, herbaceous, rarely leathery.

叶片通常披针形的到卵形，很少线形的或钻形，草质，很少革质。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Bulb ovoid-globose or globose, 5--8 mm in diam.; tunic dark brown, subleathery; bulbels several, within tunic, narrowly ovoid.

直径的鳞茎卵球状球形的或球状，5-8毫米；鳞茎皮暗褐色，近革质；珠芽几，在鳞茎皮内，狭卵球形。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

Leaves with petiole 15-50 cm; leaflets 2-8 pairs; petiolules short, less than 5 mm, stout; blades abaxially becoming brown when dry, adaxially deep green, slightly shiny, first pair ovate, evidently smaller, gradually larger toward leaf apex, elliptic-ovate to narrowly elliptic, 3-20 cm, leathery, abaxially densely tomentose, adaxially hairy only on midvein and lateral veins, lateral veins ca. 10 pairs, tips not reaching margin, base broadly cuneate to rounded, margin entire, apex obtuse or rounded, rarely acute. Inflorescences compound racemose, only branched once, densely flowered on upper part of branches; bracts subulate.

叶柄15-50厘米；小叶2-8 对；小叶柄短，不到5毫米，坚固；干燥时的叶片背面的变得的棕色，正面深的绿色，稍发亮，第一对卵形，明显小，逐渐大在叶先端，椭圆状卵形的到狭椭圆形，3-20厘米，革质，背面的密被绒毛，正面有毛的只在中脉和侧脉上，花序复总状，只是分枝一次，在分枝的上半部分上的花密集；苞片钻形。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。