印迹

It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.

在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。

Progeny viroids in the indicator plant Suyo cucumber were detected by 2D-PAGE,return-PAGE-northern-blot and RT-PCR,and only HSVd can be detected from inoculated cucumber.

通过二维电泳，正反向电泳及Northern印迹杂交，RT-PCR等分子生物学方法对接种的指示植物Suyo黄瓜中的子代类病毒进行了检测，结果仅仅检测到HSVd一种类病毒。

The isotypes of the two monoclonal antibodies were identified as IgG1, and their directed epitope located in PreS1 (34-47) identified by Western blotting analysis.

并利用上述8个序列重叠的PreS1融合蛋白，通过免疫印迹的方法鉴定了它们的作用表位位于PreS1（34-47）内。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.

对浙江省1982～1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者，用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体，发现4例阳性，并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。

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转印条件可调，能在很大的分子量跨度上获得最佳转印效果*耐用的板式电极能产生强大而均一的电场，而且电极在槽内的位置灵活可换；不论运行1、2 或3 个转印夹，电极间距可调节到最小以获得最大的场强和转印效率*凝胶支架转印夹保证整个凝胶与印迹膜表面的均衡接触*转印夹的铰链设计能防止凝胶滑动，便于转印夹组装*颜色标记的转印夹及电极板确保在转印槽内的正确定向*特级冷却芯和冷却水循环器进行温度调节―可理想地用于天然酶或高强度转印，或用于延长转印时间时减少缓冲液的损耗*可选择的组装盘是凝胶三明治和转印夹组装的理想选择 Trans-Blot Plus 转印槽能从单向和双向大格式凝胶上均一、高效地转移蛋白―配合使用新推出的 PowerPac HC 电源，多数蛋白都可在1530 分钟内完成转印。

Asked a little Green Lizard, as he ran past him with his tail in the air.

现在我总算看见他了，他的头发黑得像风信子花，他的嘴唇就像他想要的玫瑰那样红﹔但是感情的折磨使他脸色苍白如象牙，忧伤的印迹也爬上了他的眉梢。

The complexes formed between MAP and MAA were evaluated by 1H NMR,FTIR and UV spectrometry.

在预聚合阶段，通过1H NMR，FTIR和UV光谱法研究了分子印迹聚合物的形成机理。

The molecularly imprinted polymeric microspheres for L -methyldopa were synthesized by precipitation polymerization.

采用沉淀聚合法合成了左旋甲基多巴( L -Methyldopa， LMD)分子印迹聚合物微球，并对其合成条件进行了详细研究。

Wisdom cannot constitute a history; it leaves no trace in time and space; it is but pieces of wreckages; it cannot be preserved permanently; and however much, it does not compose an article or a book by itself.

智慧属于所有的时代，智慧不能形成历史，智慧无时空印迹，智慧是一块块碎片，智慧不能永久保存，智慧再多也不成篇章，这就是《博弈圣经》——一个无限的科学宝藏，里面有说不清的科学谜团。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测：利用已经克隆的美国柑桔裂皮类病毒cDNA序列，制备生物素标记的核苷酸探针，采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测，通过胶片曝光显示杂交信号，发现在两种方法的检测结果中阳性材料都有强的反应，而阴性材料无反应或较弱，该方法安全可靠、快速准确，适合大量样品的检测。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。