印迹

The results showed that ①after the treatment with 0. 3% Triton X-100 5 min or standard binding buffer 20 min,the proteins on NCM could be detected with first specific antiserum from the titer 1:100 diluted to 1: 1000;②the blocking effect of Tween 20 was more effective than that of other blockings;③both HRP-labelled conjugate and colloid gold-labelled c...

结果表明：①经（TritonX－100）＝0.3％，5min和SBB20min的蛋白质复性处理后，可使第一免疫血清稀释度1：100改为1：1000使用；②应用（Tween20）＝0.05％作为封闭液效果最好；③金标法及酶标法都可用于Western印迹技术，酶标法主带清晰，而金标法灵敏度高。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

Molecularly imprinted polymers are a new kind of macromolecular materials with molecular recognition capability. Because MIPs have the advantages of predetermination, specificity and practicability, they have been extensively used in chromatographic separation, antibody mimic, solid-phase extraction, biosensors, catalysing reaction and other relevant fields.

分子印迹聚合物是一种具有分子识别能力的新型高分子材料，由于它具有预定性、专一性和实用性等优点，因此在色谱分离、抗体摸拟、固相萃取、生物传感器及模拟酶催化等诸多领域都有广阔的应用前景。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Additionally, abnormal imprinting occurs in mouse embryonic stem cells and in clonally derived animals.

另外异常印迹可发生在鼠胚胎肝细胞中和与其来源相同的动物中。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

blood serums of the crowd are collected, antibody corresponded to the four virus examined by elisa、pa and colloidal gold strip. western blot is the confirmation test of positive for antibody of hiv. result: in the 3852 cases of paid blood donor there are 7 curriers of hiv are detected and they are all paid blood donor in past.

采集有偿献血员的血清，通过酶联免疫吸附实验、明胶颗粒凝集实验和胶体金实验来检测血清中的这四种传染病病原体的相应抗体，抗－hiv阳性确认实验是蛋白印迹实验。

AIM: To investigate the immunoblot changeS of dystrophin expression in mdx mice after bone marrow stem cells transplantation.

目的：Western blot免疫印迹分析骨髓干细胞移植后mdx鼠抗肌萎缩蛋白表达的变化。

In this study, we investigated whether calcium-dependent kinases were involved in ERKs cascade activation after global cerebral ischemia.

方法采用四动脉结扎诱导大鼠前脑缺血，用免疫印迹的方法观察几个钙依赖性蛋白激酶含量及活性的变化。

The PEA gene product was detected in the medium with Western blot, which indicated that the PEA gene with original signal peptide could be expressed with secretary form in eucaryotic cells.

表达产物经蛋白免疫印迹法（Western-blot）检测，表明本文构建的含有原基因信号肽的PEA基因能够在真核细胞进行分泌性表达，这为基于PEA的免疫毒素、疫苗佐剂和疫苗载体的研究奠定了基础。

At the rear was an innovative combination transaxle with the gearbox, clutch, differential, and inboard-mounted drum brakes.

在后面是与变速箱，离合器，差的创新结合变速，和内侧安装鼓式制动器。

For more people can apprecaite and have the master work, there is reproduction painting appears in the market, the large various selection of paint,the high saturation of color plus the plentiful sfumato, to make the reproduction perfect copy and detail as original painting, so as to meet the requirment of amateurs who love art paintings,they do not need to pay high price to get classical paintings.

颜料色域广，色彩饱和度高，色彩层次丰富，图像色彩还原真实，能完美的复制原作，满足艺术爱好者欣赏，艺术爱好者们不用支付昂贵的价格就能拥有经典油画。