印迹

The sensitivity of chemiluminescent detection is very high, and it can be used to detect single copy gene in genomic Southern blotting hybridization.

化学发光技术检测敏感性高，可用于基因组Southern印迹杂交检测单拷贝基因。

We had to extract recombine vectors to electrophoresis, prepare Biotin-probe to Dot Hybridization, check Nucleotides sequence etal.

此杂交过程除了重组子的质粒提取和DNA斑点印迹的手工制备以外，其余杂交步骤与第一部分相同。

RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果：分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体；在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

Results: The titer of Ad-PKG Ⅱ was 5×l0^9 pfu/ml. The PKG Ⅱ protein expression in Ad-PKG Ⅱ infected CS-54 cells was significantly increased in comparison with that in the control cells; in gastric cancer line BGC-823, PKG Ⅱ significantly inhibited the EGF induced activation of ERK.

结果：重组腺病毒滴度达5×10^9pfu/ml，蛋白质印迹法检测显示重组腺病毒感染CS54细胞后使其PKG Ⅱ表达明显增高；在BGC-823胃癌细胞株，PKG Ⅱ对EGF导致的ERK的激活有明显抑制作用。

Nucleus changes in apoptotic cells were investigated by using Hoechst 33258 staining and photofluorography. Apoptotic rate was evaluated by propidium iodide staining and flow cytometer.

应用Hoechst 33258染色法观察凋亡细胞的形态学改变；PI染色流式细胞仪检测细胞凋亡率；Western印迹法检测Bcl-2蛋白的表达。

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量；罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位；超氧化物歧化酶检测试剂盒检测SOD活性；免疫印迹法检测血红素氧合酶－1(HO-1)的表达。

This is because the mind retains impressions of the death experience from many previous incarnations.

这是因为人心之中仍留有许多前世生命的死亡体验之印迹。

No stamp pad, inkpad, can be repeated oiling, continuous stamping, imprinting is not clear deformation; that do cover that is easy to carry and other characteristics.

无需印台、印泥，可反复注油，连续盖印、印迹清晰不变形；即盖即干，方便携带等特色。

In the present study, CHL cells were cultured routinely in MEM medium for 3 days. Then the cells were treated with sham exposure , 0.4mT 50 Hz sinusoidal MF , 0.4mT noise MF and the combined noise and 50Hz MF for 3 min and 15min, respectively. After exposure, the cells were lysed, and the proteins were extracted.

实验中，将中国仓鼠肺成纤维细胞分别经0.4mT工频磁场、等强度的噪声磁场、工频磁场与噪声磁场的复合磁场辐照及假辐照处理3min和15min后，裂解细胞，提取细胞蛋白质，以Western印迹技术，测定并比较细胞内SAPK磷酸化的变化。

Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.

大量提取纯化重组真核表达质粒pVAX1-GRA8，瞬时转染培养的vero E-6细胞，RT-PCR和免疫印迹法检测GRA8在细胞中的表达。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。