分离密度

The research of the characteristics of biology and the osteogenic and adopocitic induction are comparatively mature, showing many biological characteristics such as adherence, plasticity, heterology, self-renewal, rapid clone formation and transplantation. Adherence combined with density gradient centrifugation method is widely used in the isolation and purification of BMSCs.

骨髓间充质干细胞现阶段研究方向主要包括生物学特性、诱导分化和调控机制等内容，对其生物学特性和成骨成脂诱导方面研究比较成熟，在培养分化过程中展示出贴壁性、可塑性、异质性、自我更新能力、快速形成克隆能力、可移植性等生物学特征，贴壁法联合密度梯度离心法在骨髓间充质干细胞的分离、纯化过程中被广泛采用。

Bone marrow was extracted from ilium of goat and gradient-centrifuged to obtain the third-passaged bone marrow mesenchyme stem cells.

羊髂骨穿刺取骨髓，梯度密度离心法分离获得第3代骨髓间充质干细胞。

Test results show that the bed material＇ s density is the main influencing factor. When sand is taken as the bed material, light and easily inflammable objects such as wood, paraffin, rubber etc can get evenly mixed in the bed, while heavy incombustible matter can adequately be segregated.

结果表明：密度是主要影响因素；以砂子为床料的内循环流化床，能够实现木块、石蜡、橡胶等轻质可烯物的均匀混合，同时灰渣等重质不可燃物能够充分分离。

The present study analysis whether CRP could directly induce inflammation in endothelial cells, analysis what kind of effects CRP provide on cellular signal transduction in endothelial cell by gene array, and isolate LDL by one step of density gradient centrifugation, generate E-LDL by degrading LDL with multiple enzymes, direct fluorescent labeling CRP and LDL, investigate whether CRP play as intermediator in atherosclerosis.

本课题目的在于探讨CRP在脂质参与的动脉粥样硬化形成中是否起到介质作用，主要通过检测直接CRP能否诱导内皮细胞炎症表现，基因芯片分析CRP对内皮系统的细胞信号转导通路的影响，探讨CRP参与调控内皮系统炎症过程的主要机制，并且通过一步法密度梯度离心分离LDL，复合酶处理生成E-LDL，直接荧光标记CRP和LDL，分析CRP介导脂质紊乱尤其是LDL代谢产物参与动脉粥样硬化早期形成的作用。

As nuclear warfare became prevalent upon Earth and upon other dimensions within the Great Central Sun, time became separated causing the separation of past, present and future.

当核战争在地球和大中枢太阳中的其它密度中盛行时，时间被分离并导致过去、现在和未来的区分。

An SCP powder called Provesteen, now on the market, is produced from a yeast that grows at densities similar to those achieved by centrifugation with ordinary strains. This means not only that centrifugation can be omitted, but also that there is no excess water to be recycled; the yeast goes right from the fermenter to a pasteurizer and a drier.

一种叫Provesteen的SCP粉末现在已进入市场，它能在一种从普通酵母离心分离后才能达到的密度条件下生长，这就意味着，它不仅可省略掉离心步骤，而且也不需要回收过量的水；这些酵母直接从发酵罐到巴氏消毒器和干燥器。

The main products are: Hammer, thermal conductivity detector, permeameter, contraction of the expansion device, hand-held strain gauge, waterproof material detector, low-bending device, slag ball content in separators, heat load tester, performance test combustion furnace, a variety of thickness, thermal expansion of graphite electrode NG, coal strength meter, electric tamping device, hydrophobicity tester, mineral wool density meter, concrete drilling sampling machines, sawing machines, removed machine, setstube machine, hydraulic bending machine, impervious tester, determination of heat resistance, heating telescopic measuring device, an extension device, penetration tester, small meter, viscometer, drying time tester, pencil hardness tester, pendulum hardness, the adhesion tester, film zoned grid, and resistant to scrubbing detector, solvent resistance detector, wearable device, flat grinding device, wear-resistant coating shakeout device, staining resistance tester, impact tester , piercing instrument, instrument bending film, film coating, and sanding machines, cone-shaped grinding, high-speed dispersion machine, three roller grinder, Scratch tester, indentation tester, gloss Miriam, white tester, fresh video of Miriam, Miriam reflectivity, iron cobalt colorimeter, paint density cup, crack resistance testing apparatus, leveling device, flow hanging device, visual color box, the minimum temperature detector into a grinding, low temperature brittleness tester, visual color boxes, electronic pull machine, Mooney Viscometer, vulcanizing device, the proportion of direct-reading, the cold compression tester, heritage wearable device, Heritage scratch device, the friction coefficient detector, coating Detect zoned grid plate , coating resistance tester, such as burning a variety of test equipment.

主要产品有：回弹仪、导热系数测定仪、渗透仪、收缩膨胀仪、手持应变仪、防水材料检测仪、低温弯折仪、渣球含量分离器、热荷重试验仪、燃烧性能试验炉、各种测厚仪、石墨电极热膨胀仪、煤强度测定仪、电动击实仪、憎水性测试仪、矿棉容重测定仪、混凝土钻孔取样机、锯切机、磨平机、套丝切管机、液压弯管机、不透水试验仪、耐热性测定具、加热伸缩测定器、延伸仪、针入度仪、细度计、粘度计、干燥时间试验器、铅笔硬度计、摆式硬度计、附着力测试仪、漆膜划格器、耐洗刷测定仪、耐溶剂测定仪、耐磨仪、平磨仪、涂层落砂耐磨仪、耐沾污测试仪、冲击试验仪、穿孔仪、漆膜弯曲仪、涂膜涂布器、砂磨机、锥形磨、高速分散机、三滚研磨机、划痕试验仪、压痕试验仪、光泽仪、白度仪、鲜映性仪、反射率仪、铁钴比色计、涂料密度杯、抗裂性试验仪、流平仪、流挂仪、目视比色箱、最低成磨温度测定仪、低温脆性试验仪、目视比色箱、电子拉力机、门尼粘度仪、硫化仪、直读比重计、压缩耐寒试验仪、文物耐磨仪、文物划痕仪、摩擦系数测定仪、涂层检测划格板、涂层耐燃烧试验仪等多种试验仪器。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

Results:Main imaging features of APK were as follows:On abdominal plain films showed the lobalate renal enlargement in 46 cases,calcification of polycystic wall in 14 cases,renal calcium milk in 5 cases,60 cases were showed separation,deform and longthend of renal pelvis on intravenous urography and retrograde urography,5 cases were showed renal enlargement on retroperitoneal pneumography,on CT scans in 25 cases showed mulltiply cysts with thin wall,unecho liquid shadows were showed on B-mode ultrasound.

结果：成人多囊肾的主要影像学征象：腹部平片(46例)显示肾影呈分叶状增大；囊壁钙化(14例)；多囊肾合并肾钙乳(5例)；肾盂造影(60例)，表现为肾盂、肾盏相互分离、变形、拉长；腹膜后充气造影(5例)：表现为肾影增大肾影与邻近器官之间的关系，其界限清晰可辨；CT扫描(25例)：表现为肾内多数大小不等的薄壁圆形低密度区；B型超声(47例)表现为肾内多个类圆形无回声的液性暗区。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。