分离密度

Methods: Density gradient centrifugalization and absorption technique wererespectively applied to detach peripheral blood mononuclear cells andperipheral blood lymphocytes,T lymphocytes were got by Nylon postabsorption method, and CD4+T and CD8+T lymphocytes divided with anti- CD4 andanti-CD8 antibody by alexin cytotoxic method.

密度梯度离心法分离外周血单个核细胞，玻璃器皿吸附法分离外周血淋巴细胞，尼龙棉柱法分离 T 淋巴细胞，补体介导的细胞毒法分别制备 CD4+T和 CD8+T 淋巴细胞，采用 T 淋巴细胞与角质形成细胞共培养技术研究银屑病患者外周血 T 细胞对角质形成细胞的影响，免疫组化法检测共培养角质形成细胞 Ki67、c-Myc 及 Bcl-xL 蛋白的表达。

Simplier and more efficient ion extraction methods for atomic vapor laser isotope separation AVLIS were evaluated using a 2-D electron-equilibrium model to compute and compare three electrostatic methods the parallel electrode method, the Π type electrode method, and the improved M type electrode method.

为探索用于原子蒸气激光同位素分离AVLIS的简单有效的离子引出方法，用二维流体理论电子平衡模型计算比较了3种静电场方法平行板电场法，Π型电极法，改进M型电极法计算发现，二维流体理论电子平衡模型并不能很好地描述Π型电极法对另外两种静电场方法，计算了不同外加电压、不同的初始原子密度和不同的目标同位素丰度等条件下的离子引出情况从计算结果可以看出，改进M型电极法不论是从离子引出时间还是从碰撞损失率，都要比平行板电极法好得多当外加电压较高或初始原子密度较高时，这种好处就更加明显因此，改进M型电极法是一种可供实际采用的比较好的静电场方法

Using IPG-DALT method, electrophoresis provide belier analysis for the lens protein separation, but the data repeatability should be further improved.

用IPG-DALT的方法，综合小样本量和低密度凝胶电泳与大样本量高密度凝胶电泳结果，可以更好地分析晶状体蛋白的分离效果，但重复性还有待进一步提高。

In [1], the kernel method of nonparametric density estimation is used to estimate the score functions directly.

文献[1]利用非参数法概率密度函数估计的核函数方法，通过对源信号评价函数的直接估计，提出了一种基于概率密度函数估计的DEBBBS算法，成功地解决了非线性函数替代方法存在的不能分离杂系混合信号的问题。

Present study aims to evaluate the effect of proso millet prolamin on cholesterol metabolism in mice, by comparing the concentration of plasma total cholesterol, high-density lipoprotein cholesterol with triglyceride of mice given the diets containing casein, soy protein isolated or PMP (supplemented with 1.82%Lys and 0.23%Trp).

比较了分别饲喂酪蛋白、大豆分离蛋白、黄米醇溶蛋白小鼠的血清总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇，甘油三酯浓度和动脉硬化指数，旨在评价黄米醇溶蛋白对小鼠胆固醇代谢的特定作用。

It studies taxi passenger's daily travel behavior feature: first, it analyzes temporal distribution of them and proposes the solution of dynamic management of taxis by limiting operation period; second, through investigating the uneven spatial distribution of taxi travel demand and nonlinear correlation of travel density and population density, it reveals the shortcoming of linear matching transportion facility with population number in traditional urban transport criterion, also it questions the limited effect of the licence management for taxis, and it proposes dynamically determing taxi scale according to different traffic zones; third, through exploring the taxi travel patterns in special zones, it reveal Hong Kong citizens travel behavior in Shenzhen, which could analyze the impact of demographic feature to the travel behavior and provide scientific testimony for designing transportion service for the special group; fourth, through accurately grasping the spatial-temporal distribution of taxi travel demand, it could provide innotiative and efficient transportion service at different time and in different place, such as taxi-sharing and flexible shuttle to meet the travel demand; through pervasive data, we could inspect urban expansion and sprawl, monitor the separation of inhatitantion center and employment center and reflect the variation of population centriod and distribution; finally, it reveals how to use digital feature of inhabitant daily travel to detect abnormal condition in the city.

对出租车乘客的日常交通行为特征进行了研究：通过对出租车出行时间分布特征的把握，提出采取限时营运的方法来对出租车进行动态管理的思路；通过分析出租车出行空间分布不均匀的特征以及出行密度与人口密度之间非线性的关系，揭示了传统城市交通规范中按人数来线性配套交通基础设施的缺陷以及牌照管理对于出租车管理的有限效果，提出按照区域动态确定营运出租车规模的思路；通过特定区域出租车出行特征的分析，揭示香港居民在深圳的交通行为特征，从而分析不同人口统计特征对于交通行为的影响，为设计这个特殊群体的交通服务提供科学依据；通过对出租车出行时空分布特征精确的把握，使得我们可以在不同时间、不同地点采用创新、高效的交通服务，如合乘出租车，高级穿梭巴士来满足出行需求；通过普适数据可以监测城市的扩张和蔓延、居住中心和就业中心的分离程度以及反映人口重心与分布的变化；最后探讨了如何利用居民日常出行的数字特征来检测城市异常状态。

To deal with the problem that Electrostatic Separation of waste circuit board is high in purity, whereas relatively low in output/capacity,we developed multi-cylinder electrostatic separator and waste circuit winnower which is developed on the principle that the specific gravities of copper powder/grain and plastics are remarkable different..

另外针静电分离对废旧电路覆铜板的分离纯度高而产量比较小，我公司开发的多滚筒静电分离机和根据粉碎后的电路板中铜粉与环氧树脂粉的密度比重差距比较大的原理研制的废旧电路板气流分选机解决了分离产量问题。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Vacuum belt hydroextractor （also named fixed rubber belt vacuum filter） is a new solid-liquid separating equipment with high efficiency and continuous operation, based on introduction, assimilation and digestion of the techniques of the same products overseas. It has achieved solid-liquid separation by making full use of material gravity and vacuum suction. In terms of its structure, filtering section is arranged horizontally lengthwise, and length of filtering section can be fully used to fulfill in turn such a process covering filtering, rinsing and sucking up, with advantages of high filtering efficiency, productivity, good rinsing effect, wide application range, simple operation, stable running, convenient maintenance and service.

真空皮带脱水机也叫固定真空室式橡胶带式过滤机；是我公司在引进、消化和吸收国外同类产品技术基础上，研制的一种新型、高效、并可连续操作的固液分离机械设备，同时它也是一种充分利用物料重力和真空吸力实现固液分离的机械设备；在结构上真空区沿水平方向布置，利用真空区的长度可以连续完成过滤、洗涤、吸干等不同工艺过程，具有过滤效果高、生产能力大、洗涤效果好、应用范围广、操作简单、运行平稳、维护保养方便等优点；特别适合于过滤固相颗粒粗、密度大及滤饼需要多次洗涤的悬浮液料浆，可以广泛应用于冶金、矿山、化工、造纸、食品、制药及环保等工业部门的固液分离；尤其在湿法烟气脱硫中的石膏脱水方面有着很好的应用。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。