分离密度

The combination of density gradient centrifugation and adhere culture is an effective method to isolate BMSCs from bone marrow aspirate.

密度梯度离心与贴壁培养相结合可以提高细胞分离效率。

For heavily stocked marine fish or reef aquariums and aquarium with heavy use of protein skimming: add 1 capful (6ml) per 25gals (95l) 15 mins before feeding twice a week.

针对高密度饲养的鱼缸或珊瑚缸，以及使用强劲蛋白质分离器的鱼缸：在喂食前15分钟，每95升水加1瓶盖（6ml），每周两次。

Traditional separation method: Several years ago, as a result of the equipment condition limit, the material particle DNA separation with the CsCl balanced equilibrium density centrifuge process, the idiomorphic become generally the gradient.

传统的分离方法：数年前，由于受设备条件限制，质粒DNA的分离一般用CsCl平衡等密度离心法，自形成梯度。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

Methods: MSCs were isolated from human adult bone marrow through density gradient with Ficoll paque (1.077g/ml) and then amplified .

采用Ficoll淋巴细胞分离液(1.077g／ml)密度梯度离心法从人的骨髓中分离出骨髓间充质干细胞进行体外扩增。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

Depends on differences in the relative densities of the substances to be separated.

它是靠待分离物相对密度的不同进行分离的。

The flow direction and packing fraction in membrane contactors were also investigated. Baffles in the shell side were found to have good effect on separation and therefore set in counter flow operation of parallel mode.

此外，为更好地提高新型中空纤维膜结构填料的分离效率，研究了膜接触器中流体的流动方式和填充密度对精馏分离性能的影响，确定了壳层挡板对现有平流运行方式的改善作用，并进行了有效的挡板设计和试运行。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。