分离密度

Methods Density gradient centrifugalization and absorption technique were respectively applied to detach peripheral blood mononuclear cells and peripheral blood lymphocytes.

密度梯度离心及吸附法分离外周血单个核细胞和外周血淋巴细胞，采用抗CD4和CD8抗体分别制备CD~(8+)和CD~(4+)T细胞进行培养，应用MTT比色法及ELISA法分别检测T细胞增殖活性及培养上清液IL-2水平。

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

Bone marrow was drawn from rabbit by density gradient centrifugate in 70% Percoll fluid, the rate of cell survival was evaluated with trypan blue staining;The cells have been induced,differentiated and transfered of culture in vitro for five weeks; MSCs was seed onto BMG and continued to be cultured six weeks in vitro,then the compound was carried out to observe collagen by Masson staining.

抽取兔骨髓，用70% Percoll液，经密度梯度离心法分离MSCs，台盼蓝染色观察细胞存活率；诱导分化，传代，体外培养5周；复合BMG，继续体外培养6周，Masson染色观察胶原合成情况。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

This paper studies extracts and pure compounds from Crataegus pinnatifida and their protection and repair effects on oxidized low-density lipoprotein induced damages of Human Mammary Epithelial Cells.

对山楂中有效成分进行分离提取，并研究其保护人微血管内皮细胞抑制氧化低密度脂蛋白损伤和对损伤的修复作用。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.

第一部分：种子细胞—肌卫星细胞的原代培养及鉴定本部分通过应用改良Percoll非连续密度梯度离心法分离纯化培养新西兰兔骨骼肌干细胞—肌卫星细胞，探讨为组织工程膀胱的构建提供种子细胞的可行性。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

Methods: the present study improved isolation of LDL, undertook one-step of density gradient centrifugation, generate E-LDL by treatment LDL with multiple enzymes, direct fluorescent label CRP and LDL, and investigate whether CRP play as intermedial mediator in atherosclerosis. Experiment consists of 8 groups: control group, N-LDL group, ox-LDL group, E-LDL group, CRP+N-LDL group, CRP+ox-LDL group, CRP+E-LDL group.

本研究参考文献改进LDL提取方法，通过一次性密度梯度超速离心，分离LDL，并且通过复合酶处理以及铜离子氧化法分别生成E-LDL和ox-LDL，并直接对CRP及LDL进行荧光标记，通过与内皮细胞共孵育，利用荧光显微镜检测CRP及LDL与内皮细胞结合，实验分为8组：空白对照组、CRP组、N-LDL组、ox-LDL组、E-LDL组、CRP+N-LDL组、CRP+ox-LDL组、CRP+E-LDL组。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。