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To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的 采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗（简称重组MUC1），为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

The state equation of induction motor with G-type parameters was established and a flux observer was designed based on U-N model. The speed adaptive law was deduced by the theory of Lyapunov stability. A method of model reference adaptive speed estimation based on the full order flux observer was proposed and a scheme of induction motor direct torque control without speed sensor was studied.

建立了基于异步电机G-型参数的状态方程，给出了基于U-N模型的定子磁链观测器；基于李雅普诺夫稳定性理论推出了速度自适应律，给出了基于这种全阶磁链观测器方案的模型参考自适应速度辨识方法。

Etc, the system may be adapted by any entity without the need for particular agreements or authorization, in reference to a praetical use in a project, this thesis s focus is on designing a mobile payment engine under the circumstance of mobile payment, composed of format transformings intelligence router switchings transaction processing and data processing, etc.

结合实际的系统，本文重点讨论了移动支付引擎的设计方法，根据移动支付的环境特点，本文介绍的引擎主要由格式转换、智能路由选择、事务处理、数据处理等大量功能组成。

Strain and relaxation, a unity of opposites, cover the overall process of playing the piano from reading the music score to practicing, and finally to playing the piano without reference to the music score.

张&与&弛这一即对立又统一的矛盾贯穿于钢琴演奏的识谱、练习、熟练弹奏到在舞台上完整地背谱演奏这一过程中。

Doubtless he chooses without reference to any pre-established value, but it is unjust to tax him with caprice.

当然，他选择时永不着参照任何既定的价值，但是责备他随心所欲是不公平的。

With analysis on the control model, an improved automatic tuning method for arc-suppression coil is proposed, that is: adopting voltage feed forward control to restrain interfere of 50Hz displacement voltage, which can improve the characteristic of the injected signal significantly, make the injected signal without reference to unbalanced network and neutral displaced system. Another innovation is detecting the magnitude of neutral point signal-frequency voltage to judge the resonant state, which improves the sensitivity and accuracy of resonant judgement effectively.

基于对控制模型的分析，提出了一种新的注入变频信号的消弧线圈自动调谐方法：通过电压前馈校正控制的引入，有效抑制了50Hz工频电压对注入信号的干扰，使得注入信号不受电网不对称及中性点位移电压的影响；提出以中性点注入信号电压幅值最大做为新的谐振判据，有效提高了谐振状态判断的灵敏度和准确度。

According to the practical characteristics and parameters during hot finishing rolling process of aluminium alloy JP1235, a three-dimensional thermo-mechanical coupled elastic-plastic FEM model was established with the software Marc. And this model was used to simulate the rolling state of F1 stand without back tension. The lateral distribution of rolling force、contact friction force and other mechanic parameters were calculated. Meanwhile, simulated the rolling state considering the influence of mill tilt, and studied the distribution of the mechanics under this state, discussed the effect of mill tile on mechanic parameters and strip steering, which can provide some reference to steering control strategy.

根据JP1235铝合金板带热精轧过程的实际工艺特点和工艺参数，采用非线性有限元软件Marc，建立一个三维热力耦合弹塑性有限元模型，模拟了精轧机组第一机架（F1机架）失去后张力作用后的轧制状态，计算出轧制过程中轧制力、摩擦力等力能参数沿板宽方向上的分布情况，在此基础上，模拟初始轧辊倾斜影响下的轧制状态，并对该轧制状态下的力能参数分布情况进行研究，探讨轧辊倾斜对力能参数分布及跑偏规律的影响，为纠偏控制提供了一定的理论依据。

This Agreement shall be subject to the substantive law in force in Germany without reference to any of its conflict of law rules.

本协议对德国的法律规则的冲突之中的任何一个，将在没有叁考的在施行中受制于实质的法律。

Perfect pitch, AKA "absolute pitch," is the rare ability to name or recreate musical notes, like A or middle C without using any comparable reference.

音高辨别力，又称&绝对音高&，是指一种不借助任何对比参照，就能辨别或唱出像A音或者中央C调等音符的罕见才能。

His attitude, at any rate, stands out as an isolated accident; the Council of Troyes (867) proclaims that no bishop can be deposed without reference to the Holy See, and the Council of Douzy (871), although held under the influence of Hincmar condemns the Bishop of Laon only under reserve of the rights of the pope.

他的态度，无论如何，站在了一个孤立的事故；理事会的特鲁瓦（ 867 ）宣布，没有主教废黜可以不提及教廷，和安理会的Douzy （ 871 ），虽然下召开的影响Hincmar谴责主教拉昂只保留下的权利的教宗。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。