查询词典 vector component

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

While the returned vector is much less useful than an unstripped vector for relevance ranking, it will usually be much smaller.

虽然返回的向量比未抽除向量在相关性计算方面用处少很多，但是它通常更小。

Vector acoustic field is a very important part of sound wave, but the vector information of acoustic wave has been unwitnessed for a long time.

矢量声场时声波场的重要组成部分，但在相当长的一段时间内没有受到重视，声波的矢量信息却被忽略了。

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

Xenopsylla Cheopis important vector in Yunnan and it is main vector role in some place of Yunnan province.

致痒蚤在云南省鼠疫流行区可充当重要媒介角色，在某些地区起到了主要媒介的作用。

Results Xenopsylla Cheopis is dominant flea and wide distribution in Yunnan province.its vector efficiency is 0.241,vector index is 0.033,colony infect rate is 94.4%,bloodsucking rate is 100%.Y.pestis is detected from Xenopsylla Cheopis in the fieldwork.

结果 致痒蚤分布范围广泛，数量多，是云南省居民地区地面蚤的优势种，其媒介效能为0 。0 3 3 ，集群传疫率为94.4%，对人吸血率为10 0 %，现场调查中从该蚤检出鼠疫菌。

And now I want to add on, so I'm gonna hook the next vector on to the front of the arrow, we'll start next vector, and I will go this way, so let's see, can I do it right?

现在我要加上…我要将下一个向量钩住这个箭头的前端，我们将会开始下一个向量，我将这样做，让我们来看，我做的对么？

Wavelet packet decompresses vibration signals, and wavelet packet nodes which are sensitive to the change of state, as eigen-nodes, form a decomposition tree; the signal restructured at all eigen-nodes can be used as the fingerprint in many breaker diagnostic systems. Support vector machine with the "most important" factors at eigen-nodes as input vector classifies the states at "one to others" strategy.

首先利用小波包分解振动数据，提取状态变化敏感节点作为特征节点形成分解树，利用敏感节点重构完好状态振动信号，并以此作为当前大多断路器诊断系统中使用的指纹信号；同时提取特征节点最大系数形成特征向量，作为支持向量机的输入向量，使用&一对其余&策略进行特征分类。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The temporal segmentation localizes moving objects by comparing the motion vector of each block in each frame with the corresponding global motion vector.

为了减小分割算法的计算量，第六章根据第五章所分析的空时融合分割算法的技术难点，提出了一种基于时空补偿的空时融合分割算法。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。