查询词典 vector component

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

This research selected the strong functional Prokaryotic expression vector pET32a+ and Eucaryote expression vector pMET α A.

通过选用功能强大的原核表达载体pET32a+和真核表达载体pMET α A，构建了Ecoli。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

Taking the pcDNA 3.1 expressions vector as the frame structure, we try to set up a eukaryon expression vector.

并以pcDNA3.1表达载体为框架结构，尝试构建真核表达载体。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

Returns a vector which lists the same lexemes as the given vector, but which lacks any position or weight information.

返回一个带有一列与给出向量相同语意的向量，但是它去掉了所有的位置和权重信息。

Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation.

构建了同时含有番茄儿丁质酶基因（Chi3）和β-1，3-葡聚糖酶基因的双价抗真菌基因植物表达载体，以西瓜子叶块为外植体，采用根癌农杆菌介导法，将Chi3和Glu-Ac同时导入西瓜栽培种&中育一号&，共获得46株抗性再生植株。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

VITS and VIR are OK also vector mode shows, the method outputs the blanking signal of undee oscillograph the Z input that receives vector oscillograph.

VITS和VIR也能够矢量模式显示，办法将波形示波器的消隐旌旗灯号输出接到矢量示波器的Z输入。

Development of vector resistance to pyrethroid insecticides has been reported all over the world,which is a major obstacle to control of vector-borne diseases.

媒介昆虫的拟除虫菊酯抗性已经在世界范围内存在，是控制媒介传染病的一个主要障碍。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。