查询词典 vector component

Shandong sinder technology limited company,qingdao 266061,shandong,chinaabstract:to provide the eukaryotic expression vector carrying 6x histidine-tag and human growth hormone in expremental animal and cell culture,according to the published nucleotide sequence of the lactofericin b and human growth hormone,synthesis sequence was ligated with pmt-18 vector.

以牛乳素为目的基因，构建含有组氨酸标签、人生长激素信号肽的真核表达载体，为利用牛乳素在活体动物及培养细胞中的表达提供基因材料。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

The thesis illustrates the principle of the and its application in the system, establishes the simulation model of the vector control system of PMSM based on the EKF and successfully realizes the application of the speedless sensor in motor control. Firstly, based on the mathematic model of permanent-magnet synchronous motor, the PMSM vector control system is built. Secondly, the Extended Kalman Filter is designed for the on-line estimation of the speed and rotor position by only using measurements of the motor voltages and currents.

首先，基于PMSM的数学模型，建立了电机的矢量控制仿真模型；其次，设计了扩展卡尔曼滤波器，通过检测电机端电压、相电流对电机转速和转子位置进行在线实时估计，实现了PMSM的无传感器矢量控制；最后，在Matlab7.0环境下进行的系统仿真试验表明，该方法具有优良的转角跟踪特性和较好的转速跟踪特性，同时系统具有较强的抗负载扰动性能和较佳的控制性能。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

The main works include: The topology structure and the switching function of the TSMC,the widely employed space vector modulation strategy without zero vector at rectifier stage and the transrectification of the TSMC were introduced.

论文的主要工作包括：介绍了TSMC的拓扑结构和开关传递函数、目前普遍采用的整流级无零矢量的空间矢量调制策略以及TSMC的换流。

Fractal theory quantifies these phenomena mainly by ascertaining their fractal dimensions.Box-counting algorithm is the one most practical and also most frequently adopted method.The traditional box-counting algorithm is based on the grid document and has some serious shortcomings,such as the distortion of the image being enlarged,the trivialness of the process and the finite of the iterative degree,etc.The vector box counting algorithm developed in this paper takes vector document as the carrier and has three advantages.

传统的计盒算法是基于栅格文件的方法，由于其存在图像放大后失真、过程繁琐和迭代次数有限等缺陷，因此为了准确简单方便地进行分维计算，开发了一种以矢量文件为载体的矢量计盒算法，并详细阐述了这种算法的数据结构、处理流程和主要函数，同时以Koch曲线、骨肿瘤边界及水系证明了矢量计盒算法的准确性和优越性。

This method is tectonic on foundation of nuclear function theory special and linear space, each issue of rank nucleus of progression of the Volterra that seek solution changeover uses output observation vector to be in to beg Xierbaite the some in the space child the umbriferous problem on the space, make originally complex, hard calculative is nonlinear in the approachs a problem to be built in place compose linear space of Volterra progression of the system solve ably with the means that accumulates inside vector, gave out specific algorithm.

该方法在核函数理论基础上构造非凡线性空间，将求解Volterra级数的各阶核的新问题转换为求用输出观测向量在希尔伯特空间中某一子空间上的投影新问题，使原本复杂、难以计算的非线性系统的Volterra级数的逼近新问题在所构建的线性空间中巧妙地以向量内积的方式解决，并给出了具体算法。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。