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This investigation was to establish a simplified culture system to isolate and passage brain tumor stem cells from human medulloblastoma, observe their proliferation and differentiation, and determine the expression of normal neural stem cell antigens, CD133 and Nestin, in BTSCs. METHODS: Eleven specimens of medulloblastoma were acutely dissociated and triturated into single cells without trypsin digestion.

本研究旨在利用简化的无血清培养基和悬浮培养方法，从人髓母细胞瘤中分离培养脑肿瘤干细胞，观察其在体外的增殖、分化，鉴定其特异性标志物CD133和Nestin的表达，验证肿瘤组织切片中CD133阳性细胞的存在，为脑肿瘤干细胞的进一步研究打下基础。

Series 1156F features a high capacity performance as well as unique simplified servicing accessibility for complete unitized maintenance.

系列1156F具有大容量的特性，而且具有专利的简单化的简易维修特点，便于整体维护。

Model T145B features a high capacity performance as well as unique simplified servicing accessibility for complete unitized maintenance.

型号T145B具有高容量性能特点，而且整个装置的维修维护特别简单容易。

A simplified theoretical model has been established to describe the big yawed rod penetration into semi-infinite Rolled Homogeneous Armor targets at normal incidence, the effect of ballistic yaw is quantitatively described in the rate of penetration.

本文建立了大攻角的长杆体垂直侵彻半无限靶的简化物理模型，定量地分析了大攻角对垂直侵彻的影响。

An analytical solution of solute diffusion through soil is presented based on simplified non-linear adsorption isotherm.

粘土颗粒对溶质的吸附绝大多数属于非线性吸附，采用非线性吸附等温线表示。

Secondly, a simplified consolidation theory for unsaturated soils is suggested based on the assumption that the pore air drainage rate is a constant during the rain water infiltration.

接着又针对雨水入渗过程中孔隙气的排气特点，提出了非饱和土简化固结理论。

In addition, a simplified consolidation theory of unsaturated soils about pore air pressure was advanced.

此外，孔隙气压力的计算也是一件麻烦事，为此提出了简化计算方法。

A simplified consolidation theory for unsaturated soils was proposed based on an assumption that pore air pressure equaled to the atmosphere pressure.

基于孔隙气压力等于大气压力的假定，建议了针对非饱和土的简化固结理论。

The cracking and deformation process of unsaturated expansive soil slope due todry_wet cycling is numerically simulated by regulating the ratio of drained pore air and coefficient of permeability, based on simplified conslidation theory for unsaturated soil.

利用非饱和土简化固结理论，通过调整土体的排气率和渗透系数来数值模拟干湿循环导致的非饱和膨胀土边坡裂缝的张开闭合以及变形发展过程。

For example, a regenerative reheating furnace fluid flow and heat transfer is researched, which has simplified the flow and heat transfer of hot air stove according to its real operating conditions.The fluid flow and heat transfer of a hot air stove are numerically simulated by using finite volume method or finite difference method,which bases on the heat transfer equation,flow equation of the gas and heat balance equation.

根据热风炉的实际运行状况对蓄热式热风炉内的流动换热过程进行了合理的简化，并基于热传导方程、气体流动方程和气体的热平衡方程，建立了蓄热体的非稳态传热模型，在对模型利用有限差分法和有限容积法得到离散方程的基础上，采用C 语言编制了模拟计算程序。

We have something to do in the new stage.

在这段崭新的时期中，我们有一些要做的。

The difference between tourism decision-making under uncertainty and risky tourism decision-making is indistinctive.

其中不确定性旅游决策和风险旅游决策的界线比较模糊，两者的区分是相对的。